Differential Phytotoxic Impact of Plant Mediated Silver Nanoparticles (AgNPs) and Silver Nitrate (AgNO3) on Brassica sp.
Continuous formation and utilization of nanoparticles (NPs) have resulted into significant discharge of nanosized particles into the environment. NPs find applications in numerous products and agriculture sector, and gaining importance in recent years. In the present study, silver nanoparticles (AgN...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-10-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fpls.2017.01501/full |
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| author | Kanchan Vishwakarma Shweta Neha Upadhyay Jaspreet Singh Shiliang Liu Shiliang Liu Vijay P. Singh Sheo M. Prasad Devendra K. Chauhan Durgesh K. Tripathi Shivesh Sharma Shivesh Sharma |
| author_facet | Kanchan Vishwakarma Shweta Neha Upadhyay Jaspreet Singh Shiliang Liu Shiliang Liu Vijay P. Singh Sheo M. Prasad Devendra K. Chauhan Durgesh K. Tripathi Shivesh Sharma Shivesh Sharma |
| author_sort | Kanchan Vishwakarma |
| collection | DOAJ |
| description | Continuous formation and utilization of nanoparticles (NPs) have resulted into significant discharge of nanosized particles into the environment. NPs find applications in numerous products and agriculture sector, and gaining importance in recent years. In the present study, silver nanoparticles (AgNPs) were biosynthesized from silver nitrate (AgNO3) by green synthesis approach using Aloe vera extract. Mustard (Brassica sp.) seedlings were grown hydroponically and toxicity of both AgNP and AgNO3 (as ionic Ag+) was assessed at various concentrations (1 and 3 mM) by analyzing shoot and root length, fresh mass, protein content, photosynthetic pigments and performance, cell viability, oxidative damage, DNA degradation and enzyme activities. The results revealed that both AgNPs and AgNO3 declined growth of Brassica seedlings due to enhanced accumulation of AgNPs and AgNO3 that subsequently caused severe inhibition in photosynthesis. Further, the results showed that both AgNPs and AgNO3 induced oxidative stress as indicated by histochemical staining of superoxide radical and hydrogen peroxide that was manifested in terms of DNA degradation and cell death. Activities of antioxidants, i.e., ascorbate peroxidase (APX) and catalase (CAT) were inhibited by AgNPs and AgNO3. Interestingly, damaging impact of AgNPs was lesser than AgNO3 on Brassica seedlings which was due to lesser accumulation of AgNPs and better activities of APX and CAT, which resulted in lesser oxidative stress, DNA degradation and cell death. The results of the present study showed differential impact of AgNPs and AgNO3 on Brassica seedlings, their mode of action, and reasons for their differential impact. The results of the present study could be implied in toxicological research for designing strategies to reduce adverse impact of AgNPs and AgNO3 on crop plants. |
| first_indexed | 2024-12-11T19:21:26Z |
| format | Article |
| id | doaj.art-dd1a87c543d946e0a8496ceb90f1fe01 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-11T19:21:26Z |
| publishDate | 2017-10-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-dd1a87c543d946e0a8496ceb90f1fe012022-12-22T00:53:30ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2017-10-01810.3389/fpls.2017.01501280975Differential Phytotoxic Impact of Plant Mediated Silver Nanoparticles (AgNPs) and Silver Nitrate (AgNO3) on Brassica sp.Kanchan Vishwakarma0 Shweta1Neha Upadhyay2Jaspreet Singh3Shiliang Liu4Shiliang Liu5Vijay P. Singh6Sheo M. Prasad7Devendra K. Chauhan8Durgesh K. Tripathi9Shivesh Sharma10Shivesh Sharma11Department of Biotechnology, Motilal Nehru National Institute of Technology Allahabad, Allahabad, IndiaD D Pant Interdisciplinary Research Lab, Department of Botany, University of Allahabad, Allahabad, IndiaDepartment of Biotechnology, Motilal Nehru National Institute of Technology Allahabad, Allahabad, IndiaDepartment of Biotechnology, Motilal Nehru National Institute of Technology Allahabad, Allahabad, IndiaCollege of Landscape Architecture, Sichuan Agricultural University, Chengdu, ChinaCollege of Agriculture, Food and Natural Resources, University of Missouri, Columbia, MO, United StatesGovernment Ramanuj Pratap Singhdev Post Graduate College, Baikunthpur, IndiaRanjan Plant Physiology and Biochemistry Laboratory, Department of Botany, University of Allahabad, Allahabad, IndiaD D Pant Interdisciplinary Research Lab, Department of Botany, University of Allahabad, Allahabad, IndiaCentre for Medical Diagnostic and Research, Motilal Nehru National Institute of Technology Allahabad, Allahabad, IndiaDepartment of Biotechnology, Motilal Nehru National Institute of Technology Allahabad, Allahabad, IndiaCentre for Medical Diagnostic and Research, Motilal Nehru National Institute of Technology Allahabad, Allahabad, IndiaContinuous formation and utilization of nanoparticles (NPs) have resulted into significant discharge of nanosized particles into the environment. NPs find applications in numerous products and agriculture sector, and gaining importance in recent years. In the present study, silver nanoparticles (AgNPs) were biosynthesized from silver nitrate (AgNO3) by green synthesis approach using Aloe vera extract. Mustard (Brassica sp.) seedlings were grown hydroponically and toxicity of both AgNP and AgNO3 (as ionic Ag+) was assessed at various concentrations (1 and 3 mM) by analyzing shoot and root length, fresh mass, protein content, photosynthetic pigments and performance, cell viability, oxidative damage, DNA degradation and enzyme activities. The results revealed that both AgNPs and AgNO3 declined growth of Brassica seedlings due to enhanced accumulation of AgNPs and AgNO3 that subsequently caused severe inhibition in photosynthesis. Further, the results showed that both AgNPs and AgNO3 induced oxidative stress as indicated by histochemical staining of superoxide radical and hydrogen peroxide that was manifested in terms of DNA degradation and cell death. Activities of antioxidants, i.e., ascorbate peroxidase (APX) and catalase (CAT) were inhibited by AgNPs and AgNO3. Interestingly, damaging impact of AgNPs was lesser than AgNO3 on Brassica seedlings which was due to lesser accumulation of AgNPs and better activities of APX and CAT, which resulted in lesser oxidative stress, DNA degradation and cell death. The results of the present study showed differential impact of AgNPs and AgNO3 on Brassica seedlings, their mode of action, and reasons for their differential impact. The results of the present study could be implied in toxicological research for designing strategies to reduce adverse impact of AgNPs and AgNO3 on crop plants.http://journal.frontiersin.org/article/10.3389/fpls.2017.01501/fullAgNPsAgNO3plant growthBrassicaphotosynthetic parameters |
| spellingShingle | Kanchan Vishwakarma Shweta Neha Upadhyay Jaspreet Singh Shiliang Liu Shiliang Liu Vijay P. Singh Sheo M. Prasad Devendra K. Chauhan Durgesh K. Tripathi Shivesh Sharma Shivesh Sharma Differential Phytotoxic Impact of Plant Mediated Silver Nanoparticles (AgNPs) and Silver Nitrate (AgNO3) on Brassica sp. Frontiers in Plant Science AgNPs AgNO3 plant growth Brassica photosynthetic parameters |
| title | Differential Phytotoxic Impact of Plant Mediated Silver Nanoparticles (AgNPs) and Silver Nitrate (AgNO3) on Brassica sp. |
| title_full | Differential Phytotoxic Impact of Plant Mediated Silver Nanoparticles (AgNPs) and Silver Nitrate (AgNO3) on Brassica sp. |
| title_fullStr | Differential Phytotoxic Impact of Plant Mediated Silver Nanoparticles (AgNPs) and Silver Nitrate (AgNO3) on Brassica sp. |
| title_full_unstemmed | Differential Phytotoxic Impact of Plant Mediated Silver Nanoparticles (AgNPs) and Silver Nitrate (AgNO3) on Brassica sp. |
| title_short | Differential Phytotoxic Impact of Plant Mediated Silver Nanoparticles (AgNPs) and Silver Nitrate (AgNO3) on Brassica sp. |
| title_sort | differential phytotoxic impact of plant mediated silver nanoparticles agnps and silver nitrate agno3 on brassica sp |
| topic | AgNPs AgNO3 plant growth Brassica photosynthetic parameters |
| url | http://journal.frontiersin.org/article/10.3389/fpls.2017.01501/full |
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