Isolasi dan Karakterisasi Ralstonia syzygii
Ralstonia syzygii is one of important pathogens of cloves. This study was aimed at obtaining pure culture of R. syzygi from diseased plant tissue samples. The acterium was isolated from infected clove plantations in Ungaran, Central Java. It had the ability to make clove seedlings show symptoms and...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universitas Gadjah Mada
2009-07-01
|
| Series: | Jurnal Perlindungan Tanaman Indonesia |
| Subjects: | |
| Online Access: | https://jurnal.ugm.ac.id/jpti/article/view/11759 |
| _version_ | 1831675796089995264 |
|---|---|
| author | Yanuar Danaatmadja Siti Subandiyah Tri Joko Cavrina Ulita Sari |
| author_facet | Yanuar Danaatmadja Siti Subandiyah Tri Joko Cavrina Ulita Sari |
| author_sort | Yanuar Danaatmadja |
| collection | DOAJ |
| description | Ralstonia syzygii is one of important pathogens of cloves. This study was aimed at obtaining pure culture of R. syzygi from diseased plant tissue samples. The acterium was isolated from infected clove plantations in Ungaran, Central Java. It had the ability to make clove seedlings show symptoms and then die 41 days after inoculation. The reisolated bacterium also showed its ability to kill clove seedlings after 17 days. The bacterium is gram negative, able to ultilize glucose, sucrose, maltose, mannitol, sorbitol, dulcitol, and glycerol, able to hydrolyze starch, grow well at 27oC, able to catalyze and release water and oxygen from hydrogen peroxyde (H2O2) and aerobically.
Ralstonia syzygii merupakan salah satu patogen penting pada tanaman cengkeh. Penelitian ini dilakukan untuk mendapakan isolat murni R syzygii dari jaringan tanaman terinfeksi. Bakteri diisolasi dari pertanaman cengkeh sakit yang berasal dari daerah Ungaran, Jawa Tengah. Bakteri menunjukkan kemampuan untuk menyebabkan bibit tanaman cangkeh sakit dan kemudian mati 41 hsi. Bakteri hasil reisolasi juga menunjukkan kemampuannya menyebabkan sakit dan kematian pada bibit tanaman cengkeh setelah 17 hsi. Bakteri yang diperoleh bersifat gram negatif, mampu memanfaatkan glukosa, sukrosa, maltosa, manitol, sorbitol, dulsitol, dan gliserol, mampu menghidrolisis pati, tumbuh baik pada suhu 27o C, mampu mengkatalisis dan melepaskan air dan oksigen dari hydrogen peroxide (H2O2) dan bersifat aerob. |
| first_indexed | 2024-12-20T04:10:57Z |
| format | Article |
| id | doaj.art-dd22574bb9cb43dba348a0faccbfbcbf |
| institution | Directory Open Access Journal |
| issn | 1410-1637 2548-4788 |
| language | English |
| last_indexed | 2024-12-20T04:10:57Z |
| publishDate | 2009-07-01 |
| publisher | Universitas Gadjah Mada |
| record_format | Article |
| series | Jurnal Perlindungan Tanaman Indonesia |
| spelling | doaj.art-dd22574bb9cb43dba348a0faccbfbcbf2022-12-21T19:53:55ZengUniversitas Gadjah MadaJurnal Perlindungan Tanaman Indonesia1410-16372548-47882009-07-0115171210.22146/jpti.117599235Isolasi dan Karakterisasi Ralstonia syzygiiYanuar Danaatmadja0Siti Subandiyah1Tri Joko2Cavrina Ulita Sari3Program Studi Fitopatologi Pascasarjana Fakultas Pertanian Universitas Gadjah MadaFakultas Pertanian Universitas Gadjah MadaFakultas Pertanian Universitas Gadjah MadaPT Cengkeh ZanzibarRalstonia syzygii is one of important pathogens of cloves. This study was aimed at obtaining pure culture of R. syzygi from diseased plant tissue samples. The acterium was isolated from infected clove plantations in Ungaran, Central Java. It had the ability to make clove seedlings show symptoms and then die 41 days after inoculation. The reisolated bacterium also showed its ability to kill clove seedlings after 17 days. The bacterium is gram negative, able to ultilize glucose, sucrose, maltose, mannitol, sorbitol, dulcitol, and glycerol, able to hydrolyze starch, grow well at 27oC, able to catalyze and release water and oxygen from hydrogen peroxyde (H2O2) and aerobically. Ralstonia syzygii merupakan salah satu patogen penting pada tanaman cengkeh. Penelitian ini dilakukan untuk mendapakan isolat murni R syzygii dari jaringan tanaman terinfeksi. Bakteri diisolasi dari pertanaman cengkeh sakit yang berasal dari daerah Ungaran, Jawa Tengah. Bakteri menunjukkan kemampuan untuk menyebabkan bibit tanaman cangkeh sakit dan kemudian mati 41 hsi. Bakteri hasil reisolasi juga menunjukkan kemampuannya menyebabkan sakit dan kematian pada bibit tanaman cengkeh setelah 17 hsi. Bakteri yang diperoleh bersifat gram negatif, mampu memanfaatkan glukosa, sukrosa, maltosa, manitol, sorbitol, dulsitol, dan gliserol, mampu menghidrolisis pati, tumbuh baik pada suhu 27o C, mampu mengkatalisis dan melepaskan air dan oksigen dari hydrogen peroxide (H2O2) dan bersifat aerob.https://jurnal.ugm.ac.id/jpti/article/view/11759clove seedlingRalstonia syzygiibibit cengkeh |
| spellingShingle | Yanuar Danaatmadja Siti Subandiyah Tri Joko Cavrina Ulita Sari Isolasi dan Karakterisasi Ralstonia syzygii Jurnal Perlindungan Tanaman Indonesia clove seedling Ralstonia syzygii bibit cengkeh |
| title | Isolasi dan Karakterisasi Ralstonia syzygii |
| title_full | Isolasi dan Karakterisasi Ralstonia syzygii |
| title_fullStr | Isolasi dan Karakterisasi Ralstonia syzygii |
| title_full_unstemmed | Isolasi dan Karakterisasi Ralstonia syzygii |
| title_short | Isolasi dan Karakterisasi Ralstonia syzygii |
| title_sort | isolasi dan karakterisasi ralstonia syzygii |
| topic | clove seedling Ralstonia syzygii bibit cengkeh |
| url | https://jurnal.ugm.ac.id/jpti/article/view/11759 |
| work_keys_str_mv | AT yanuardanaatmadja isolasidankarakterisasiralstoniasyzygii AT sitisubandiyah isolasidankarakterisasiralstoniasyzygii AT trijoko isolasidankarakterisasiralstoniasyzygii AT cavrinaulitasari isolasidankarakterisasiralstoniasyzygii |