Expression of Autophagy-Related Factors LC3A and Beclin 1 and Apoptosis-Related Factors Bcl-2 and BAX in Osteoblasts Treated With Sodium Fluoride
ObjectiveThis study aims to analyze the expressions of autophagy-related factors light chain 3 alpha (LC3A) and Beclin 1 and apoptosis-related factors B-cell lymphoma 2 (Bcl-2) and Bcl-2-associated X (BAX) in primary osteoblasts treated with sodium fluoride (NaF).MethodsOsteoblasts were extracted fr...
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Frontiers Media S.A.
2021-07-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2021.603848/full |
| _version_ | 1818602659787046912 |
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| author | Lin Xu Lin Xu Chaonan Deng Chaonan Deng Ying Zhang Lina Zhao Yan Linghu Yan Linghu Yanni Yu Yanni Yu |
| author_facet | Lin Xu Lin Xu Chaonan Deng Chaonan Deng Ying Zhang Lina Zhao Yan Linghu Yan Linghu Yanni Yu Yanni Yu |
| author_sort | Lin Xu |
| collection | DOAJ |
| description | ObjectiveThis study aims to analyze the expressions of autophagy-related factors light chain 3 alpha (LC3A) and Beclin 1 and apoptosis-related factors B-cell lymphoma 2 (Bcl-2) and Bcl-2-associated X (BAX) in primary osteoblasts treated with sodium fluoride (NaF).MethodsOsteoblasts were extracted from Sprague-Dawley rats and treated with 0, 2.5, 5, and 10 mg/L NaF solutions, followed by 10 mmol/L 3-methyladenine (3-MA) for 24 h. The apoptotic rate was determined by flow cytometry, and the expressions of the autophagy- and apoptosis-related factors were measured by western blotting and real-time quantitative polymerase chain reaction.ResultsThe mRNA expressions of LC3A, Beclin 1, and BAX in the NaF-treated osteoblast group were higher than those in the control group, while the protein expressions of these factors in the NaF-treated group were significantly higher than those in the control group. However, the Bcl-2 protein expression in the NaF-treated osteoblasts was significantly decreased compared to that in the control cells. After the 3-MA treatment, the protein expressions of LC3A, Beclin 1, and Bcl-2 were significantly decreased compared with those of the NaF-treated group, whereas the expression of BAX increased. Moreover, the apoptosis rate was increased after the addition of the 3-MA inhibitor.ConclusionNaF stimulation promoted autophagy and apoptosis of the osteoblasts, suggesting the involvement of fluoride damage in these processes. |
| first_indexed | 2024-12-16T13:10:48Z |
| format | Article |
| id | doaj.art-dd2472f3429d4facab4d2184ee4799ec |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-12-16T13:10:48Z |
| publishDate | 2021-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-dd2472f3429d4facab4d2184ee4799ec2022-12-21T22:30:38ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2021-07-011210.3389/fphys.2021.603848603848Expression of Autophagy-Related Factors LC3A and Beclin 1 and Apoptosis-Related Factors Bcl-2 and BAX in Osteoblasts Treated With Sodium FluorideLin Xu0Lin Xu1Chaonan Deng2Chaonan Deng3Ying Zhang4Lina Zhao5Yan Linghu6Yan Linghu7Yanni Yu8Yanni Yu9Guiyang Maternal and Child Health Care Hospital of Guiyang, Guiyang, ChinaDepartment of Pathology, The Affiliated Hospital of Guizhou Medical University, Guiyang, ChinaDepartment of Pathology, The Affiliated Hospital of Guizhou Medical University, Guiyang, ChinaDepartment of Pathology, Guizhou Medical University, Guiyang, ChinaGuiyang Maternal and Child Health Care Hospital of Guiyang, Guiyang, ChinaDepartment of Pathology, The Affiliated Hospital of Guizhou Medical University, Guiyang, ChinaDepartment of Pathology, The Affiliated Hospital of Guizhou Medical University, Guiyang, ChinaDepartment of Pathology, Guizhou Medical University, Guiyang, ChinaDepartment of Pathology, The Affiliated Hospital of Guizhou Medical University, Guiyang, ChinaDepartment of Pathology, Guizhou Medical University, Guiyang, ChinaObjectiveThis study aims to analyze the expressions of autophagy-related factors light chain 3 alpha (LC3A) and Beclin 1 and apoptosis-related factors B-cell lymphoma 2 (Bcl-2) and Bcl-2-associated X (BAX) in primary osteoblasts treated with sodium fluoride (NaF).MethodsOsteoblasts were extracted from Sprague-Dawley rats and treated with 0, 2.5, 5, and 10 mg/L NaF solutions, followed by 10 mmol/L 3-methyladenine (3-MA) for 24 h. The apoptotic rate was determined by flow cytometry, and the expressions of the autophagy- and apoptosis-related factors were measured by western blotting and real-time quantitative polymerase chain reaction.ResultsThe mRNA expressions of LC3A, Beclin 1, and BAX in the NaF-treated osteoblast group were higher than those in the control group, while the protein expressions of these factors in the NaF-treated group were significantly higher than those in the control group. However, the Bcl-2 protein expression in the NaF-treated osteoblasts was significantly decreased compared to that in the control cells. After the 3-MA treatment, the protein expressions of LC3A, Beclin 1, and Bcl-2 were significantly decreased compared with those of the NaF-treated group, whereas the expression of BAX increased. Moreover, the apoptosis rate was increased after the addition of the 3-MA inhibitor.ConclusionNaF stimulation promoted autophagy and apoptosis of the osteoblasts, suggesting the involvement of fluoride damage in these processes.https://www.frontiersin.org/articles/10.3389/fphys.2021.603848/fullautophagyapoptosisosteoblastslight chain 3 alphaBeclin 1B-cell lymphoma 2 |
| spellingShingle | Lin Xu Lin Xu Chaonan Deng Chaonan Deng Ying Zhang Lina Zhao Yan Linghu Yan Linghu Yanni Yu Yanni Yu Expression of Autophagy-Related Factors LC3A and Beclin 1 and Apoptosis-Related Factors Bcl-2 and BAX in Osteoblasts Treated With Sodium Fluoride Frontiers in Physiology autophagy apoptosis osteoblasts light chain 3 alpha Beclin 1 B-cell lymphoma 2 |
| title | Expression of Autophagy-Related Factors LC3A and Beclin 1 and Apoptosis-Related Factors Bcl-2 and BAX in Osteoblasts Treated With Sodium Fluoride |
| title_full | Expression of Autophagy-Related Factors LC3A and Beclin 1 and Apoptosis-Related Factors Bcl-2 and BAX in Osteoblasts Treated With Sodium Fluoride |
| title_fullStr | Expression of Autophagy-Related Factors LC3A and Beclin 1 and Apoptosis-Related Factors Bcl-2 and BAX in Osteoblasts Treated With Sodium Fluoride |
| title_full_unstemmed | Expression of Autophagy-Related Factors LC3A and Beclin 1 and Apoptosis-Related Factors Bcl-2 and BAX in Osteoblasts Treated With Sodium Fluoride |
| title_short | Expression of Autophagy-Related Factors LC3A and Beclin 1 and Apoptosis-Related Factors Bcl-2 and BAX in Osteoblasts Treated With Sodium Fluoride |
| title_sort | expression of autophagy related factors lc3a and beclin 1 and apoptosis related factors bcl 2 and bax in osteoblasts treated with sodium fluoride |
| topic | autophagy apoptosis osteoblasts light chain 3 alpha Beclin 1 B-cell lymphoma 2 |
| url | https://www.frontiersin.org/articles/10.3389/fphys.2021.603848/full |
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