TEKSTİL ATIK SUYUNDA HRP-CLEA İLE HİDROJEN PEROKSİDİN GİDERİLMESİ İÇİN ÖLÇEK BÜYÜTME TASARIMININ MODELLENMESİ VE SİMÜLASYONU
Hydrogen peroxide is a strong oxidant chemical used asa bleach in the textile industry and it is important to remove it from water before being released to the environment. The oxidizing substrate of peroxidase (HRP) enzyme is hydrogen peroxide. In this study, cross-linked aggregates...
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| Format: | Article |
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Eskişehir Osmangazi University
2021-04-01
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| Series: | Eskişehir Osmangazi Üniversitesi Mühendislik ve Mimarlık Fakültesi Dergisi |
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| Online Access: | https://dergipark.org.tr/en/download/article-file/1301944 |
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| author | Gülçin ÖZEVCİ Oğuz AKPOLAT Hakan AYHAN Fatma AYHAN |
| author_facet | Gülçin ÖZEVCİ Oğuz AKPOLAT Hakan AYHAN Fatma AYHAN |
| author_sort | Gülçin ÖZEVCİ |
| collection | DOAJ |
| description | Hydrogen peroxide is a strong oxidant chemical used asa bleach in the textile industry and it is important to remove it from water before being released to the environment. The oxidizing substrate of peroxidase (HRP) enzyme is hydrogen peroxide. In this study, cross-linked aggregates (CLEA) of HRP enzyme were used to remove hydrogen peroxide and glutaraldehyde (GA) was used as a crosslinking agent.When the kinetic constants of the free peroxidase enzyme were calculated, saturation constant was 0,06965 mmol H2O2/L and maximum specific rate constant was found as 0.0232mmol H2O2/L.sec. Average saturation constant, Kmof HRP-CLEA was found as 0,00625 mmol H2O2/L, and average maximum specific rate constant value, Vm, was calculated as 0.0699x10-3 mmol H2O2/L.sec in wastewater without dye. More then 90 % H2O2removal was achieved in 15 minutes with HRP-CLEA. The removal of the wastewater containing H2O2(0.02M) was completed in five minutes.In the scale-up approach, the data was evaluated using the Euler solution algorithm and the H2O2removal in the efficiency factor range where the kinetic constants change was calculated. Assuming the reaction rate changes linearly, the activity variations that will occur during scaling up with the enzyme in aggregate form were determined in the range of 125% -50%, and the industrial-scale design was modelled by taking into account the activity factors. In scaling up, concentrations that will occur in the increased reaction volume have been simulated. The results obtained showed that HRP-CLEAs are more advantageous than free enzyme in terms of time and cost. |
| first_indexed | 2024-04-10T14:55:57Z |
| format | Article |
| id | doaj.art-dd257dbd95af4cdc964b407b399f8b3e |
| institution | Directory Open Access Journal |
| issn | 2630-5712 2630-5712 |
| language | English |
| last_indexed | 2024-04-10T14:55:57Z |
| publishDate | 2021-04-01 |
| publisher | Eskişehir Osmangazi University |
| record_format | Article |
| series | Eskişehir Osmangazi Üniversitesi Mühendislik ve Mimarlık Fakültesi Dergisi |
| spelling | doaj.art-dd257dbd95af4cdc964b407b399f8b3e2023-02-15T16:07:21ZengEskişehir Osmangazi UniversityEskişehir Osmangazi Üniversitesi Mühendislik ve Mimarlık Fakültesi Dergisi2630-57122630-57122021-04-012918696https://doi.org/10.31796/ogummf.797983TEKSTİL ATIK SUYUNDA HRP-CLEA İLE HİDROJEN PEROKSİDİN GİDERİLMESİ İÇİN ÖLÇEK BÜYÜTME TASARIMININ MODELLENMESİ VE SİMÜLASYONUGülçin ÖZEVCİ0https://orcid.org/0000-0003-1375-7443Oğuz AKPOLAT1https://orcid.org/0000-0002-6623-4323Hakan AYHAN2https://orcid.org/0000-0003-2911-1655Fatma AYHAN3https://orcid.org/0000-0003-2220-4496stanbul Sağlık ve Teknoloji Üniversitesi, Sağlık Hizmetleri MeslekYüksekokulu, Tıbbi Hizmetler ve Teknikler Bölümü, Tıbbi Görüntüleme Teknikleri ProgramıMuğla Sıtkı Koçman Universitesi, Fen Fakültesi, Kimya BölümüMuğla Sıtkı Koçman Universitesi, Fen Fakültesi, Kimya Bölümü, Biyokimya Anabilim Dalı, Biyokimya & Biyomalzemeler Araştırma GrubuMuğla Sıtkı Koçman Universitesi, Fen Fakültesi, Kimya Bölümü, Biyokimya Anabilim Dalı, Biyokimya & Biyomalzemeler Araştırma GrubuHydrogen peroxide is a strong oxidant chemical used asa bleach in the textile industry and it is important to remove it from water before being released to the environment. The oxidizing substrate of peroxidase (HRP) enzyme is hydrogen peroxide. In this study, cross-linked aggregates (CLEA) of HRP enzyme were used to remove hydrogen peroxide and glutaraldehyde (GA) was used as a crosslinking agent.When the kinetic constants of the free peroxidase enzyme were calculated, saturation constant was 0,06965 mmol H2O2/L and maximum specific rate constant was found as 0.0232mmol H2O2/L.sec. Average saturation constant, Kmof HRP-CLEA was found as 0,00625 mmol H2O2/L, and average maximum specific rate constant value, Vm, was calculated as 0.0699x10-3 mmol H2O2/L.sec in wastewater without dye. More then 90 % H2O2removal was achieved in 15 minutes with HRP-CLEA. The removal of the wastewater containing H2O2(0.02M) was completed in five minutes.In the scale-up approach, the data was evaluated using the Euler solution algorithm and the H2O2removal in the efficiency factor range where the kinetic constants change was calculated. Assuming the reaction rate changes linearly, the activity variations that will occur during scaling up with the enzyme in aggregate form were determined in the range of 125% -50%, and the industrial-scale design was modelled by taking into account the activity factors. In scaling up, concentrations that will occur in the increased reaction volume have been simulated. The results obtained showed that HRP-CLEAs are more advantageous than free enzyme in terms of time and cost.https://dergipark.org.tr/en/download/article-file/1301944hr peroxidasecross-linked enzyme aggregatescarrier-free enzyme immobilizationmodellingscaling up |
| spellingShingle | Gülçin ÖZEVCİ Oğuz AKPOLAT Hakan AYHAN Fatma AYHAN TEKSTİL ATIK SUYUNDA HRP-CLEA İLE HİDROJEN PEROKSİDİN GİDERİLMESİ İÇİN ÖLÇEK BÜYÜTME TASARIMININ MODELLENMESİ VE SİMÜLASYONU Eskişehir Osmangazi Üniversitesi Mühendislik ve Mimarlık Fakültesi Dergisi hr peroxidase cross-linked enzyme aggregates carrier-free enzyme immobilization modelling scaling up |
| title | TEKSTİL ATIK SUYUNDA HRP-CLEA İLE HİDROJEN PEROKSİDİN GİDERİLMESİ İÇİN ÖLÇEK BÜYÜTME TASARIMININ MODELLENMESİ VE SİMÜLASYONU |
| title_full | TEKSTİL ATIK SUYUNDA HRP-CLEA İLE HİDROJEN PEROKSİDİN GİDERİLMESİ İÇİN ÖLÇEK BÜYÜTME TASARIMININ MODELLENMESİ VE SİMÜLASYONU |
| title_fullStr | TEKSTİL ATIK SUYUNDA HRP-CLEA İLE HİDROJEN PEROKSİDİN GİDERİLMESİ İÇİN ÖLÇEK BÜYÜTME TASARIMININ MODELLENMESİ VE SİMÜLASYONU |
| title_full_unstemmed | TEKSTİL ATIK SUYUNDA HRP-CLEA İLE HİDROJEN PEROKSİDİN GİDERİLMESİ İÇİN ÖLÇEK BÜYÜTME TASARIMININ MODELLENMESİ VE SİMÜLASYONU |
| title_short | TEKSTİL ATIK SUYUNDA HRP-CLEA İLE HİDROJEN PEROKSİDİN GİDERİLMESİ İÇİN ÖLÇEK BÜYÜTME TASARIMININ MODELLENMESİ VE SİMÜLASYONU |
| title_sort | tekstil atik suyunda hrp clea ile hidrojen peroksidin giderilmesi icin olcek buyutme tasariminin modellenmesi ve simulasyonu |
| topic | hr peroxidase cross-linked enzyme aggregates carrier-free enzyme immobilization modelling scaling up |
| url | https://dergipark.org.tr/en/download/article-file/1301944 |
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