Detection of Helicobacter pylori genes (CagA and VacA) in municipal drinking water
Background: Helicobacter pylori is classified as a carcinogen, and it is also the most common cause of chronic bacterial infection and peptic ulcers. Approximately 45% of people are infected with the bacterium. Methods: In this study, the H. pylori genes, CagA and VacA, were investigated in drink...
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| Format: | Article |
| Language: | English |
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Kerman University of Medical Sciences
2022-09-01
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| Series: | Environmental Health Engineering and Management |
| Subjects: | |
| Online Access: | http://ehemj.com/article-1-1006-en.html |
| _version_ | 1811272168139390976 |
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| author | Fathollah Gholami-Borujeni Hamzeh Rahimi Seyed Mehdi Sajjadi |
| author_facet | Fathollah Gholami-Borujeni Hamzeh Rahimi Seyed Mehdi Sajjadi |
| author_sort | Fathollah Gholami-Borujeni |
| collection | DOAJ |
| description | Background: Helicobacter pylori is classified as a carcinogen, and it is also the most common cause
of chronic bacterial infection and peptic ulcers. Approximately 45% of people are infected with the
bacterium.
Methods: In this study, the H. pylori genes, CagA and VacA, were investigated in drinking water, using
100 samples (50 samples from the municipal water supply and 50 samples from the effluent of household
water treatment devices). DNA was extracted from colonies with a positive heterotrophic plate count
(HPC) for use in molecular testing and microbial identification. The polymerase chain reaction (PCR)
was used to identify H. pylori.
Results: The study showed that 24% of urban water samples (12% above the World Health Organization
[WHO] standards for safe drinking water) and 18% of home water treatment-device samples (4% above
the WHO standards) were HPC-positive. The H. pylori genes, CagA and VacA, were identified in 2%
of the samples from household water treatment devices and 8% of the municipal water supply samples.
Conclusion: The study findings show that H. pylori may be transmitted in drinking water. However,
there is currently no strong evidence that the bacteria can survive after the disinfection process in the
water supply system. Therefore, the health risks of this bacterium in drinking water are still unknown. |
| first_indexed | 2024-04-12T22:35:26Z |
| format | Article |
| id | doaj.art-dd25dfdfbfd8481e97078fa276b48be5 |
| institution | Directory Open Access Journal |
| issn | 2423-3765 2423-4311 |
| language | English |
| last_indexed | 2024-04-12T22:35:26Z |
| publishDate | 2022-09-01 |
| publisher | Kerman University of Medical Sciences |
| record_format | Article |
| series | Environmental Health Engineering and Management |
| spelling | doaj.art-dd25dfdfbfd8481e97078fa276b48be52022-12-22T03:13:53ZengKerman University of Medical SciencesEnvironmental Health Engineering and Management2423-37652423-43112022-09-019330531010.34172/EHEM.2022.31Detection of Helicobacter pylori genes (CagA and VacA) in municipal drinking waterFathollah Gholami-Borujeni0Hamzeh Rahimi1Seyed Mehdi Sajjadi2Department of Environmental Health Engineering, Faculty of Health, Mazandaran University of Medical Sciences, Sari, IranDepartment of Molecular Medicine, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, IranCellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand, IranBackground: Helicobacter pylori is classified as a carcinogen, and it is also the most common cause of chronic bacterial infection and peptic ulcers. Approximately 45% of people are infected with the bacterium. Methods: In this study, the H. pylori genes, CagA and VacA, were investigated in drinking water, using 100 samples (50 samples from the municipal water supply and 50 samples from the effluent of household water treatment devices). DNA was extracted from colonies with a positive heterotrophic plate count (HPC) for use in molecular testing and microbial identification. The polymerase chain reaction (PCR) was used to identify H. pylori. Results: The study showed that 24% of urban water samples (12% above the World Health Organization [WHO] standards for safe drinking water) and 18% of home water treatment-device samples (4% above the WHO standards) were HPC-positive. The H. pylori genes, CagA and VacA, were identified in 2% of the samples from household water treatment devices and 8% of the municipal water supply samples. Conclusion: The study findings show that H. pylori may be transmitted in drinking water. However, there is currently no strong evidence that the bacteria can survive after the disinfection process in the water supply system. Therefore, the health risks of this bacterium in drinking water are still unknown.http://ehemj.com/article-1-1006-en.htmlhelicobacter pyloridrinking waterpolymerase chain reactionwater supplywater purification |
| spellingShingle | Fathollah Gholami-Borujeni Hamzeh Rahimi Seyed Mehdi Sajjadi Detection of Helicobacter pylori genes (CagA and VacA) in municipal drinking water Environmental Health Engineering and Management helicobacter pylori drinking water polymerase chain reaction water supply water purification |
| title | Detection of Helicobacter pylori genes (CagA and VacA) in municipal drinking water |
| title_full | Detection of Helicobacter pylori genes (CagA and VacA) in municipal drinking water |
| title_fullStr | Detection of Helicobacter pylori genes (CagA and VacA) in municipal drinking water |
| title_full_unstemmed | Detection of Helicobacter pylori genes (CagA and VacA) in municipal drinking water |
| title_short | Detection of Helicobacter pylori genes (CagA and VacA) in municipal drinking water |
| title_sort | detection of helicobacter pylori genes caga and vaca in municipal drinking water |
| topic | helicobacter pylori drinking water polymerase chain reaction water supply water purification |
| url | http://ehemj.com/article-1-1006-en.html |
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