Evaluation of the phytotoxic effect of extracts from endophytic fungi Colletotrichum dianesei and Xylaria sp. isolated from Palicourea corymbifera (Rubiaceae)
The allelopathic action can be verified by the presence of phytotoxic substances, molecules that are directed to the agribusiness sector. The objective of this study was to evaluate the chemical composition of the methanolic extracts from Colletotrichum dianesei and Xylaria sp., and phytotoxic poten...
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| Format: | Article |
| Language: | English |
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Universidad de la República
2020-12-01
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| Series: | Agrociencia |
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| Online Access: | http://agrocienciauruguay.uy/ojs/index.php/agrociencia/article/view/423/418 |
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| author | Weison Lima Da Silva Laísley Martins Lima Cecilia Veronica Nunez |
| author_facet | Weison Lima Da Silva Laísley Martins Lima Cecilia Veronica Nunez |
| author_sort | Weison Lima Da Silva |
| collection | DOAJ |
| description | The allelopathic action can be verified by the presence of phytotoxic substances, molecules that are directed to the agribusiness sector. The objective of this study was to evaluate the chemical composition of the methanolic extracts from Colletotrichum dianesei and Xylaria sp., and phytotoxic potential on Lactuca sativa. The fungi were grown in Sabouraud liquid medium supplemented with 0.2% yeast extract, under stirring. The mycelia were subjected to solvent extraction in order of increasing polarity, but methanol extracts were used in this investigation. The allelopathic activity was evaluated at a concentration of 1000 µg/mL in quadruplicate, evaluating: percentage and speed of germination, and growth of lettuce seedlings under laboratory conditions. C. dianesei extract reduced germination percentage by 36% and delayed germination speed by 63.8%, while Xylaria sp. didn't interfere with the germination percentage, it only delayed the germination rate by 43.9%. Root growth was negatively affected in 30.5% and 51.1% under the influence of extracts from Xylaria sp. and C. dianesei, there was also a reduction of 33.1 and 31.8% in the aerial part length, respectively. The thin layer chromatography showed the presence of aromatic substances and terpenes in both extracts, and alkaloids only in C. dianesei extract. The 1H nmr spectra showed signals between 6 and 8.5 ppm (aromatic substances), being relatively more intense for Xylaria sp., signals between 3 and 4 ppm (sugars, showing anomers only for Xylaria sp.) and methyl groups between 0.7 and 1.3 ppm, suggesting terpenes. The results suggest that the fungal species evaluated have phytotoxic potential, and produce terpenes and aromatic substances. |
| first_indexed | 2024-12-18T11:50:02Z |
| format | Article |
| id | doaj.art-dd288fcaf55b41efa06a84de92f75555 |
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| issn | 2301-1548 |
| language | English |
| last_indexed | 2024-12-18T11:50:02Z |
| publishDate | 2020-12-01 |
| publisher | Universidad de la República |
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| spelling | doaj.art-dd288fcaf55b41efa06a84de92f755552022-12-21T21:09:11ZengUniversidad de la RepúblicaAgrociencia2301-15482020-12-0124NE21910.31285/AGRO.24.423Evaluation of the phytotoxic effect of extracts from endophytic fungi Colletotrichum dianesei and Xylaria sp. isolated from Palicourea corymbifera (Rubiaceae)Weison Lima Da Silva0https://orcid.org/0000-0002-8908-8576Laísley Martins Lima1https://orcid.org/0000-0003-1447-3661Cecilia Veronica Nunez2https://orcid.org/0000-0003-3400-9508Instituto Nacional de Pesquisas da Amazônia (INPA), Coordenação de Tecnologia e Inovação (COTI), Laboratório de Bioprospecção e Biotecnologia (LABB), Manaus, Amazonas, Brazil.Instituto Nacional de Pesquisas da Amazônia (INPA), Coordenação de Tecnologia e Inovação (COTI), Laboratório de Bioprospecção e Biotecnologia (LABB), Manaus, Amazonas, Brazil.Instituto Nacional de Pesquisas da Amazônia (INPA), Coordenação de Tecnologia e Inovação (COTI), Laboratório de Bioprospecção e Biotecnologia (LABB), Manaus, Amazonas, Brazil.The allelopathic action can be verified by the presence of phytotoxic substances, molecules that are directed to the agribusiness sector. The objective of this study was to evaluate the chemical composition of the methanolic extracts from Colletotrichum dianesei and Xylaria sp., and phytotoxic potential on Lactuca sativa. The fungi were grown in Sabouraud liquid medium supplemented with 0.2% yeast extract, under stirring. The mycelia were subjected to solvent extraction in order of increasing polarity, but methanol extracts were used in this investigation. The allelopathic activity was evaluated at a concentration of 1000 µg/mL in quadruplicate, evaluating: percentage and speed of germination, and growth of lettuce seedlings under laboratory conditions. C. dianesei extract reduced germination percentage by 36% and delayed germination speed by 63.8%, while Xylaria sp. didn't interfere with the germination percentage, it only delayed the germination rate by 43.9%. Root growth was negatively affected in 30.5% and 51.1% under the influence of extracts from Xylaria sp. and C. dianesei, there was also a reduction of 33.1 and 31.8% in the aerial part length, respectively. The thin layer chromatography showed the presence of aromatic substances and terpenes in both extracts, and alkaloids only in C. dianesei extract. The 1H nmr spectra showed signals between 6 and 8.5 ppm (aromatic substances), being relatively more intense for Xylaria sp., signals between 3 and 4 ppm (sugars, showing anomers only for Xylaria sp.) and methyl groups between 0.7 and 1.3 ppm, suggesting terpenes. The results suggest that the fungal species evaluated have phytotoxic potential, and produce terpenes and aromatic substances.http://agrocienciauruguay.uy/ojs/index.php/agrociencia/article/view/423/418phytotoxicitybioprospectingendophytic |
| spellingShingle | Weison Lima Da Silva Laísley Martins Lima Cecilia Veronica Nunez Evaluation of the phytotoxic effect of extracts from endophytic fungi Colletotrichum dianesei and Xylaria sp. isolated from Palicourea corymbifera (Rubiaceae) Agrociencia phytotoxicity bioprospecting endophytic |
| title | Evaluation of the phytotoxic effect of extracts from endophytic fungi Colletotrichum dianesei and Xylaria sp. isolated from Palicourea corymbifera (Rubiaceae) |
| title_full | Evaluation of the phytotoxic effect of extracts from endophytic fungi Colletotrichum dianesei and Xylaria sp. isolated from Palicourea corymbifera (Rubiaceae) |
| title_fullStr | Evaluation of the phytotoxic effect of extracts from endophytic fungi Colletotrichum dianesei and Xylaria sp. isolated from Palicourea corymbifera (Rubiaceae) |
| title_full_unstemmed | Evaluation of the phytotoxic effect of extracts from endophytic fungi Colletotrichum dianesei and Xylaria sp. isolated from Palicourea corymbifera (Rubiaceae) |
| title_short | Evaluation of the phytotoxic effect of extracts from endophytic fungi Colletotrichum dianesei and Xylaria sp. isolated from Palicourea corymbifera (Rubiaceae) |
| title_sort | evaluation of the phytotoxic effect of extracts from endophytic fungi colletotrichum dianesei and xylaria sp isolated from palicourea corymbifera rubiaceae |
| topic | phytotoxicity bioprospecting endophytic |
| url | http://agrocienciauruguay.uy/ojs/index.php/agrociencia/article/view/423/418 |
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