Identification of MicroRNAs as Viable Aggressiveness Biomarkers for Prostate Cancer
MiRNAs play a relevant role in PC (prostate cancer) by the regulation in the expression of several pathways’ AR (androgen receptor), cellular cycle, apoptosis, MET (mesenchymal epithelium transition), or metastasis. Here, we report the role of several miRNAs’ expression patterns, such as miR-93-5p,...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-06-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/9/6/646 |
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| author | Luis Javier Martínez-González Victor Sánchez-Conde Jose María González-Cabezuelo Alba Antunez-Rodríguez Eduardo Andrés-León Inmaculada Robles-Fernandez Jose Antonio Lorente Fernando Vázquez-Alonso María Jesus Alvarez-Cubero |
| author_facet | Luis Javier Martínez-González Victor Sánchez-Conde Jose María González-Cabezuelo Alba Antunez-Rodríguez Eduardo Andrés-León Inmaculada Robles-Fernandez Jose Antonio Lorente Fernando Vázquez-Alonso María Jesus Alvarez-Cubero |
| author_sort | Luis Javier Martínez-González |
| collection | DOAJ |
| description | MiRNAs play a relevant role in PC (prostate cancer) by the regulation in the expression of several pathways’ AR (androgen receptor), cellular cycle, apoptosis, MET (mesenchymal epithelium transition), or metastasis. Here, we report the role of several miRNAs’ expression patterns, such as miR-93-5p, miR-23c, miR-210-3p, miR-221-3p, miR-592, miR-141, miR-375, and miR-130b, with relevance in processes like cell proliferation and MET. Using Trizol<sup>®</sup> extraction protocol and TaqMan™ specific probes for amplification, we performed miRNAs’ analysis of 159 PC fresh tissues and 60 plasmas from peripheral blood samples. We had clinical data from all samples including PSA, Gleason, TNM, and D’Amico risk. Moreover, a bioinformatic analysis in TCGA (The Cancer Genome Atlas) was included to analyze the effect of the most relevant miRNAs according to aggressiveness in an extensive cohort (<i>n</i> = 531). We found that miR-210-3p, miR-23c, miR-592, and miR-93-5p are the most suitable biomarkers for PC aggressiveness and diagnosis, respectively. In fact, according with our results, miR-93-5p seems the most promising non-invasive biomarker for PC. To sum up, miR-210-3p, miR-23c, miR-592, and miR-93-5p miRNAs are suggested to be potential biomarkers for PC risk stratification that could be included in non-invasive strategies such as liquid biopsy in precision medicine for PC management. |
| first_indexed | 2024-03-10T10:41:27Z |
| format | Article |
| id | doaj.art-dd2bceca90be43be9639cd9700a6d986 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-10T10:41:27Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-dd2bceca90be43be9639cd9700a6d9862023-11-21T22:56:20ZengMDPI AGBiomedicines2227-90592021-06-019664610.3390/biomedicines9060646Identification of MicroRNAs as Viable Aggressiveness Biomarkers for Prostate CancerLuis Javier Martínez-González0Victor Sánchez-Conde1Jose María González-Cabezuelo2Alba Antunez-Rodríguez3Eduardo Andrés-León4Inmaculada Robles-Fernandez5Jose Antonio Lorente6Fernando Vázquez-Alonso7María Jesus Alvarez-Cubero8GENYO. Centre for Genomics and Oncological Research: Pfizer, University of Granada, Andalusian Regional Government, Genomics Unit, PTS Granada-Avenida de la Ilustración, 114-18016 Granada, SpainUrology Department, Hospital Virgen de las Nieves, 18014 Granada, SpainResearch and Development Department, Meridiem Seeds, 04710 Almería, SpainGENYO. Centre for Genomics and Oncological Research: Pfizer, University of Granada, Andalusian Regional Government, Genomics Unit, PTS Granada-Avenida de la Ilustración, 114-18016 Granada, SpainBioinformatics Unit, Institute of Parasitology and Biomedicine “López-Neyra” (IPBLN), Spanish National Research Council (CSIC), 18016 Granada, SpainGENYO. Centre for Genomics and Oncological Research: Pfizer, University of Granada, Andalusian Regional Government, Liquid Biopsy and Cancer Interception Group, PTS Granada, 114-18016 Granada, SpainGENYO. Centre for Genomics and Oncological Research: Pfizer, University of Granada, Andalusian Regional Government, Liquid Biopsy and Cancer Interception Group, PTS Granada, 114-18016 Granada, SpainUrology Department, Hospital Virgen de las Nieves, 18014 Granada, SpainGENYO. Centre for Genomics and Oncological Research: Pfizer, University of Granada, Andalusian Regional Government, Liquid Biopsy and Cancer Interception Group, PTS Granada, 114-18016 Granada, SpainMiRNAs play a relevant role in PC (prostate cancer) by the regulation in the expression of several pathways’ AR (androgen receptor), cellular cycle, apoptosis, MET (mesenchymal epithelium transition), or metastasis. Here, we report the role of several miRNAs’ expression patterns, such as miR-93-5p, miR-23c, miR-210-3p, miR-221-3p, miR-592, miR-141, miR-375, and miR-130b, with relevance in processes like cell proliferation and MET. Using Trizol<sup>®</sup> extraction protocol and TaqMan™ specific probes for amplification, we performed miRNAs’ analysis of 159 PC fresh tissues and 60 plasmas from peripheral blood samples. We had clinical data from all samples including PSA, Gleason, TNM, and D’Amico risk. Moreover, a bioinformatic analysis in TCGA (The Cancer Genome Atlas) was included to analyze the effect of the most relevant miRNAs according to aggressiveness in an extensive cohort (<i>n</i> = 531). We found that miR-210-3p, miR-23c, miR-592, and miR-93-5p are the most suitable biomarkers for PC aggressiveness and diagnosis, respectively. In fact, according with our results, miR-93-5p seems the most promising non-invasive biomarker for PC. To sum up, miR-210-3p, miR-23c, miR-592, and miR-93-5p miRNAs are suggested to be potential biomarkers for PC risk stratification that could be included in non-invasive strategies such as liquid biopsy in precision medicine for PC management.https://www.mdpi.com/2227-9059/9/6/646aggressivenessbiomarkersbioinformaticprecision medicineprostate cancer |
| spellingShingle | Luis Javier Martínez-González Victor Sánchez-Conde Jose María González-Cabezuelo Alba Antunez-Rodríguez Eduardo Andrés-León Inmaculada Robles-Fernandez Jose Antonio Lorente Fernando Vázquez-Alonso María Jesus Alvarez-Cubero Identification of MicroRNAs as Viable Aggressiveness Biomarkers for Prostate Cancer Biomedicines aggressiveness biomarkers bioinformatic precision medicine prostate cancer |
| title | Identification of MicroRNAs as Viable Aggressiveness Biomarkers for Prostate Cancer |
| title_full | Identification of MicroRNAs as Viable Aggressiveness Biomarkers for Prostate Cancer |
| title_fullStr | Identification of MicroRNAs as Viable Aggressiveness Biomarkers for Prostate Cancer |
| title_full_unstemmed | Identification of MicroRNAs as Viable Aggressiveness Biomarkers for Prostate Cancer |
| title_short | Identification of MicroRNAs as Viable Aggressiveness Biomarkers for Prostate Cancer |
| title_sort | identification of micrornas as viable aggressiveness biomarkers for prostate cancer |
| topic | aggressiveness biomarkers bioinformatic precision medicine prostate cancer |
| url | https://www.mdpi.com/2227-9059/9/6/646 |
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