Respiratory Colonization and Short-Term Temporal Changes in the Urinary Metabolome of Children
The human metabolome may vary based on age, over time, and in the presence of viral carriage and bacterial colonization—a common scenario in children. We used nuclear magnetic resonance spectroscopy to identify and quantify urinary metabolites of children without signs or symptoms of respiratory ill...
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| Format: | Article |
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MDPI AG
2021-07-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/11/8/500 |
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| author | Lilliam Ambroggio Todd A. Florin Kayla Williamson Cory Pfefferman Brandie D. Wagner Larisa Yeomans Jae Hyun Kim Heidi Sucharew Maurizio Macaluso Richard M. Ruddy Samir S. Shah Kathleen A. Stringer |
| author_facet | Lilliam Ambroggio Todd A. Florin Kayla Williamson Cory Pfefferman Brandie D. Wagner Larisa Yeomans Jae Hyun Kim Heidi Sucharew Maurizio Macaluso Richard M. Ruddy Samir S. Shah Kathleen A. Stringer |
| author_sort | Lilliam Ambroggio |
| collection | DOAJ |
| description | The human metabolome may vary based on age, over time, and in the presence of viral carriage and bacterial colonization—a common scenario in children. We used nuclear magnetic resonance spectroscopy to identify and quantify urinary metabolites of children without signs or symptoms of respiratory illness. A urine sample and two nasopharyngeal swabs were collected to test for respiratory viral pathogens and colonization by <i>Streptococcus pneumoniae</i> (<i>Sp</i>). Urine samples were collected at the initial visit, 24 h post-enrollment, and 10–14 days post-enrollment. Of the 122 children enrolled, 24% had a virus detected and 19.7% had <i>Sp</i> detected. Intraclass correlation coefficients demonstrated greater within-subject versus between-subject variability for all metabolites detected. In linear mixed models adjusted for age, time, history of asthma, <i>Sp</i>, and viruses, 1-methylnicotinamide was increased by 50% in children with <i>Sp</i> and decreased by 35% in children with rhinovirus/enterovirus. Children with <i>Sp</i> had 83% higher levels of trimethylamine-N-oxide compared with those without <i>Sp</i>. However, when adjusting for multiple comparisons, the association was no longer statistically significant. In conclusion, there appear to be short-term changes within the urinary metabolome of healthy children, but levels of metabolites did not statistically differ in children with viral carriage or <i>Sp</i> detected. |
| first_indexed | 2024-03-10T08:36:33Z |
| format | Article |
| id | doaj.art-dd2c6ec5e3df467a80eb9f764079b038 |
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| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-10T08:36:33Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-dd2c6ec5e3df467a80eb9f764079b0382023-11-22T08:39:11ZengMDPI AGMetabolites2218-19892021-07-0111850010.3390/metabo11080500Respiratory Colonization and Short-Term Temporal Changes in the Urinary Metabolome of ChildrenLilliam Ambroggio0Todd A. Florin1Kayla Williamson2Cory Pfefferman3Brandie D. Wagner4Larisa Yeomans5Jae Hyun Kim6Heidi Sucharew7Maurizio Macaluso8Richard M. Ruddy9Samir S. Shah10Kathleen A. Stringer11Sections of Emergency Medicine and Hospital Medicine, Children’s Hospital Colorado & Department of Pediatrics, University of Colorado, Aurora, CO 80045, USADivision of Emergency Medicine, Ann and Robert H. Lurie Children’s Hospital of Chicago & Department of Pediatrics, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USADepartment of Biostatistics and Informatics, Colorado School of Public Health, University of Colorado, Aurora, CO 80045, USADivision of Biostatistics and Epidemiology, Cincinnati Children’s Hospital Medical Center & Department of Pediatrics, College of Medicine, University of Cincinnati, Cincinnati, OH 45267, USADepartment of Biostatistics and Informatics, Colorado School of Public Health, University of Colorado, Aurora, CO 80045, USANuclear Magnetic Resonance Metabolomics Laboratory, Division of Pulmonary and Critical Care Medicine, Department of Clinical Pharmacy, College of Pharmacy, Michigan Center for Integrative Research in Critical Care, School of Medicine, University of Michigan, Ann Arbor, MI 48109, USANuclear Magnetic Resonance Metabolomics Laboratory, Division of Pulmonary and Critical Care Medicine, Department of Clinical Pharmacy, College of Pharmacy, Michigan Center for Integrative Research in Critical Care, School of Medicine, University of Michigan, Ann Arbor, MI 48109, USADivision of Biostatistics and Epidemiology, Cincinnati Children’s Hospital Medical Center & Department of Pediatrics, College of Medicine, University of Cincinnati, Cincinnati, OH 45267, USADivision of Biostatistics and Epidemiology, Cincinnati Children’s Hospital Medical Center & Department of Pediatrics, College of Medicine, University of Cincinnati, Cincinnati, OH 45267, USADivision of Emergency Medicine, Cincinnati Children’s Hospital Medical Center & Department of Pediatrics, College of Medicine, University of Cincinnati, Cincinnati, OH 45267, USADivisions of Hospital Medicine and Infectious Diseases, Cincinnati Children’s Hospital Medical Center & Department of Pediatrics, College of Medicine, University of Cincinnati, Cincinnati, OH 45267, USANuclear Magnetic Resonance Metabolomics Laboratory, Division of Pulmonary and Critical Care Medicine, Department of Clinical Pharmacy, College of Pharmacy, Michigan Center for Integrative Research in Critical Care, School of Medicine, University of Michigan, Ann Arbor, MI 48109, USAThe human metabolome may vary based on age, over time, and in the presence of viral carriage and bacterial colonization—a common scenario in children. We used nuclear magnetic resonance spectroscopy to identify and quantify urinary metabolites of children without signs or symptoms of respiratory illness. A urine sample and two nasopharyngeal swabs were collected to test for respiratory viral pathogens and colonization by <i>Streptococcus pneumoniae</i> (<i>Sp</i>). Urine samples were collected at the initial visit, 24 h post-enrollment, and 10–14 days post-enrollment. Of the 122 children enrolled, 24% had a virus detected and 19.7% had <i>Sp</i> detected. Intraclass correlation coefficients demonstrated greater within-subject versus between-subject variability for all metabolites detected. In linear mixed models adjusted for age, time, history of asthma, <i>Sp</i>, and viruses, 1-methylnicotinamide was increased by 50% in children with <i>Sp</i> and decreased by 35% in children with rhinovirus/enterovirus. Children with <i>Sp</i> had 83% higher levels of trimethylamine-N-oxide compared with those without <i>Sp</i>. However, when adjusting for multiple comparisons, the association was no longer statistically significant. In conclusion, there appear to be short-term changes within the urinary metabolome of healthy children, but levels of metabolites did not statistically differ in children with viral carriage or <i>Sp</i> detected.https://www.mdpi.com/2218-1989/11/8/500pediatrichealthymetabolomenuclear magnetic resonance<i>Streptococcus pneumoniae</i>rhinovirus/enterovirus |
| spellingShingle | Lilliam Ambroggio Todd A. Florin Kayla Williamson Cory Pfefferman Brandie D. Wagner Larisa Yeomans Jae Hyun Kim Heidi Sucharew Maurizio Macaluso Richard M. Ruddy Samir S. Shah Kathleen A. Stringer Respiratory Colonization and Short-Term Temporal Changes in the Urinary Metabolome of Children Metabolites pediatric healthy metabolome nuclear magnetic resonance <i>Streptococcus pneumoniae</i> rhinovirus/enterovirus |
| title | Respiratory Colonization and Short-Term Temporal Changes in the Urinary Metabolome of Children |
| title_full | Respiratory Colonization and Short-Term Temporal Changes in the Urinary Metabolome of Children |
| title_fullStr | Respiratory Colonization and Short-Term Temporal Changes in the Urinary Metabolome of Children |
| title_full_unstemmed | Respiratory Colonization and Short-Term Temporal Changes in the Urinary Metabolome of Children |
| title_short | Respiratory Colonization and Short-Term Temporal Changes in the Urinary Metabolome of Children |
| title_sort | respiratory colonization and short term temporal changes in the urinary metabolome of children |
| topic | pediatric healthy metabolome nuclear magnetic resonance <i>Streptococcus pneumoniae</i> rhinovirus/enterovirus |
| url | https://www.mdpi.com/2218-1989/11/8/500 |
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