Comparative Transcriptome Analysis of Grafted Tomato with Drought Tolerance
Grafting is a method used in agriculture to improve crop production and tolerance to biotic and abiotic stress. This technique is widely used in tomato, <i>Solanum lycopersicum</i> L.; however, the effects of grafting on changes in gene expression associated with stress tolerance in shoo...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-07-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/15/1947 |
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| author | Maria Isabel Fuentes-Merlos Masaru Bamba Shusei Sato Atsushi Higashitani |
| author_facet | Maria Isabel Fuentes-Merlos Masaru Bamba Shusei Sato Atsushi Higashitani |
| author_sort | Maria Isabel Fuentes-Merlos |
| collection | DOAJ |
| description | Grafting is a method used in agriculture to improve crop production and tolerance to biotic and abiotic stress. This technique is widely used in tomato, <i>Solanum lycopersicum</i> L.; however, the effects of grafting on changes in gene expression associated with stress tolerance in shoot apical meristem cells are still under-discovered. To clarify the effect of grafting, we performed a transcriptomic analysis between non-grafted and grafted tomatoes using the tomato variety Momotaro-scion and rootstock varieties, TD1, GS, and GF. Drought tolerance was significantly improved not only by a combination of compatible resistant rootstock TD1 but also by self-grafted compared to non-grafted lines. Next, we found the differences in gene expression between grafted and non-grafted plants before and during drought stress treatment. These altered genes are involved in the regulation of plant hormones, stress response, and cell proliferation. Furthermore, when comparing compatible (Momo/TD1 and Momo/Momo) and incompatible (Momo/GF) grafted lines, the incompatible line reduced gene expression associated with phytohormones but increased in wounding and starvation stress-response genes. These results conclude that grafting generates drought stress tolerance through several gene expression changes in the apical meristem. |
| first_indexed | 2024-03-09T10:06:54Z |
| format | Article |
| id | doaj.art-dd2d345e7ade43078a4b489b9c832ccd |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T10:06:54Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-dd2d345e7ade43078a4b489b9c832ccd2023-12-01T23:06:06ZengMDPI AGPlants2223-77472022-07-011115194710.3390/plants11151947Comparative Transcriptome Analysis of Grafted Tomato with Drought ToleranceMaria Isabel Fuentes-Merlos0Masaru Bamba1Shusei Sato2Atsushi Higashitani3Graduate School of Life Sciences, Tohoku University, Sendai 980-8577, JapanGraduate School of Life Sciences, Tohoku University, Sendai 980-8577, JapanGraduate School of Life Sciences, Tohoku University, Sendai 980-8577, JapanGraduate School of Life Sciences, Tohoku University, Sendai 980-8577, JapanGrafting is a method used in agriculture to improve crop production and tolerance to biotic and abiotic stress. This technique is widely used in tomato, <i>Solanum lycopersicum</i> L.; however, the effects of grafting on changes in gene expression associated with stress tolerance in shoot apical meristem cells are still under-discovered. To clarify the effect of grafting, we performed a transcriptomic analysis between non-grafted and grafted tomatoes using the tomato variety Momotaro-scion and rootstock varieties, TD1, GS, and GF. Drought tolerance was significantly improved not only by a combination of compatible resistant rootstock TD1 but also by self-grafted compared to non-grafted lines. Next, we found the differences in gene expression between grafted and non-grafted plants before and during drought stress treatment. These altered genes are involved in the regulation of plant hormones, stress response, and cell proliferation. Furthermore, when comparing compatible (Momo/TD1 and Momo/Momo) and incompatible (Momo/GF) grafted lines, the incompatible line reduced gene expression associated with phytohormones but increased in wounding and starvation stress-response genes. These results conclude that grafting generates drought stress tolerance through several gene expression changes in the apical meristem.https://www.mdpi.com/2223-7747/11/15/1947tomatograftingdrought stresstranscriptomicsphytohormones |
| spellingShingle | Maria Isabel Fuentes-Merlos Masaru Bamba Shusei Sato Atsushi Higashitani Comparative Transcriptome Analysis of Grafted Tomato with Drought Tolerance Plants tomato grafting drought stress transcriptomics phytohormones |
| title | Comparative Transcriptome Analysis of Grafted Tomato with Drought Tolerance |
| title_full | Comparative Transcriptome Analysis of Grafted Tomato with Drought Tolerance |
| title_fullStr | Comparative Transcriptome Analysis of Grafted Tomato with Drought Tolerance |
| title_full_unstemmed | Comparative Transcriptome Analysis of Grafted Tomato with Drought Tolerance |
| title_short | Comparative Transcriptome Analysis of Grafted Tomato with Drought Tolerance |
| title_sort | comparative transcriptome analysis of grafted tomato with drought tolerance |
| topic | tomato grafting drought stress transcriptomics phytohormones |
| url | https://www.mdpi.com/2223-7747/11/15/1947 |
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