Whole Genome Characterization and Evolutionary Analysis of G1P[8] Rotavirus A Strains during the Pre- and Post-Vaccine Periods in Mozambique (2012–2017)
Mozambique introduced the Rotarix<sup>®</sup> vaccine (GSK Biologicals, Rixensart, Belgium) into the National Immunization Program in September 2015. Although G1P[8] was one of the most prevalent genotypes between 2012 and 2017 in Mozambique, no complete genomes had been sequenced to dat...
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| Format: | Article |
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MDPI AG
2020-12-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/9/12/1026 |
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| author | Benilde Munlela Eva D. João Celeste M. Donato Amy Strydom Simone S. Boene Assucênio Chissaque Adilson F. L. Bauhofer Jerónimo Langa Marta Cassocera Idalécia Cossa-Moiane Jorfélia J. Chilaúle Hester G. O’Neill Nilsa de Deus |
| author_facet | Benilde Munlela Eva D. João Celeste M. Donato Amy Strydom Simone S. Boene Assucênio Chissaque Adilson F. L. Bauhofer Jerónimo Langa Marta Cassocera Idalécia Cossa-Moiane Jorfélia J. Chilaúle Hester G. O’Neill Nilsa de Deus |
| author_sort | Benilde Munlela |
| collection | DOAJ |
| description | Mozambique introduced the Rotarix<sup>®</sup> vaccine (GSK Biologicals, Rixensart, Belgium) into the National Immunization Program in September 2015. Although G1P[8] was one of the most prevalent genotypes between 2012 and 2017 in Mozambique, no complete genomes had been sequenced to date. Here we report whole genome sequence analysis for 36 G1P[8] strains using an Illumina MiSeq platform. All strains exhibited a Wa-like genetic backbone (G1-P[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1). Phylogenetic analysis showed that most of the Mozambican strains clustered closely together in a conserved clade for the entire genome. No distinct clustering for pre- and post-vaccine strains were observed. These findings may suggest no selective pressure by the introduction of the Rotarix<sup>®</sup> vaccine in 2015. Two strains (HJM1646 and HGM0544) showed varied clustering for the entire genome, suggesting reassortment, whereas a further strain obtained from a rural area (MAN0033) clustered separately for all gene segments. Bayesian analysis for the VP7 and VP4 encoding gene segments supported the phylogenetic analysis and indicated a possible introduction from India around 2011.7 and 2013.0 for the main Mozambican clade. Continued monitoring of rotavirus strains in the post-vaccine period is required to fully understand the impact of vaccine introduction on the diversity and evolution of rotavirus strains. |
| first_indexed | 2024-03-09T05:59:46Z |
| format | Article |
| id | doaj.art-dd2ff209c4be400985df1d701b8b8f63 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-09T05:59:46Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-dd2ff209c4be400985df1d701b8b8f632023-12-03T12:10:26ZengMDPI AGPathogens2076-08172020-12-01912102610.3390/pathogens9121026Whole Genome Characterization and Evolutionary Analysis of G1P[8] Rotavirus A Strains during the Pre- and Post-Vaccine Periods in Mozambique (2012–2017)Benilde Munlela0Eva D. João1Celeste M. Donato2Amy Strydom3Simone S. Boene4Assucênio Chissaque5Adilson F. L. Bauhofer6Jerónimo Langa7Marta Cassocera8Idalécia Cossa-Moiane9Jorfélia J. Chilaúle10Hester G. O’Neill11Nilsa de Deus12Instituto Nacional de Saúde (INS), Distrito de Marracuene, Maputo 3943, MozambiqueInstituto Nacional de Saúde (INS), Distrito de Marracuene, Maputo 3943, MozambiqueEnteric Diseases Group, Murdoch Children’s Research Institute, 50 Flemington Road, Parkville, Melbourne 3052, AustraliaDepartment of Microbial, Biochemical and Food Biotechnology, University of the Free State, 205 Nelson Mandela Avenue, Bloemfontein 9301, South AfricaInstituto Nacional de Saúde (INS), Distrito de Marracuene, Maputo 3943, MozambiqueInstituto Nacional de Saúde (INS), Distrito de Marracuene, Maputo 3943, MozambiqueInstituto Nacional de Saúde (INS), Distrito de Marracuene, Maputo 3943, MozambiqueInstituto Nacional de Saúde (INS), Distrito de Marracuene, Maputo 3943, MozambiqueInstituto Nacional de Saúde (INS), Distrito de Marracuene, Maputo 3943, MozambiqueInstituto Nacional de Saúde (INS), Distrito de Marracuene, Maputo 3943, MozambiqueInstituto Nacional de Saúde (INS), Distrito de Marracuene, Maputo 3943, MozambiqueDepartment of Microbial, Biochemical and Food Biotechnology, University of the Free State, 205 Nelson Mandela Avenue, Bloemfontein 9301, South AfricaInstituto Nacional de Saúde (INS), Distrito de Marracuene, Maputo 3943, MozambiqueMozambique introduced the Rotarix<sup>®</sup> vaccine (GSK Biologicals, Rixensart, Belgium) into the National Immunization Program in September 2015. Although G1P[8] was one of the most prevalent genotypes between 2012 and 2017 in Mozambique, no complete genomes had been sequenced to date. Here we report whole genome sequence analysis for 36 G1P[8] strains using an Illumina MiSeq platform. All strains exhibited a Wa-like genetic backbone (G1-P[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1). Phylogenetic analysis showed that most of the Mozambican strains clustered closely together in a conserved clade for the entire genome. No distinct clustering for pre- and post-vaccine strains were observed. These findings may suggest no selective pressure by the introduction of the Rotarix<sup>®</sup> vaccine in 2015. Two strains (HJM1646 and HGM0544) showed varied clustering for the entire genome, suggesting reassortment, whereas a further strain obtained from a rural area (MAN0033) clustered separately for all gene segments. Bayesian analysis for the VP7 and VP4 encoding gene segments supported the phylogenetic analysis and indicated a possible introduction from India around 2011.7 and 2013.0 for the main Mozambican clade. Continued monitoring of rotavirus strains in the post-vaccine period is required to fully understand the impact of vaccine introduction on the diversity and evolution of rotavirus strains.https://www.mdpi.com/2076-0817/9/12/1026rotavirus group AG1P[8]whole genome sequencingRotarix<sup>®</sup>Bayesian analysisMozambique |
| spellingShingle | Benilde Munlela Eva D. João Celeste M. Donato Amy Strydom Simone S. Boene Assucênio Chissaque Adilson F. L. Bauhofer Jerónimo Langa Marta Cassocera Idalécia Cossa-Moiane Jorfélia J. Chilaúle Hester G. O’Neill Nilsa de Deus Whole Genome Characterization and Evolutionary Analysis of G1P[8] Rotavirus A Strains during the Pre- and Post-Vaccine Periods in Mozambique (2012–2017) Pathogens rotavirus group A G1P[8] whole genome sequencing Rotarix<sup>®</sup> Bayesian analysis Mozambique |
| title | Whole Genome Characterization and Evolutionary Analysis of G1P[8] Rotavirus A Strains during the Pre- and Post-Vaccine Periods in Mozambique (2012–2017) |
| title_full | Whole Genome Characterization and Evolutionary Analysis of G1P[8] Rotavirus A Strains during the Pre- and Post-Vaccine Periods in Mozambique (2012–2017) |
| title_fullStr | Whole Genome Characterization and Evolutionary Analysis of G1P[8] Rotavirus A Strains during the Pre- and Post-Vaccine Periods in Mozambique (2012–2017) |
| title_full_unstemmed | Whole Genome Characterization and Evolutionary Analysis of G1P[8] Rotavirus A Strains during the Pre- and Post-Vaccine Periods in Mozambique (2012–2017) |
| title_short | Whole Genome Characterization and Evolutionary Analysis of G1P[8] Rotavirus A Strains during the Pre- and Post-Vaccine Periods in Mozambique (2012–2017) |
| title_sort | whole genome characterization and evolutionary analysis of g1p 8 rotavirus a strains during the pre and post vaccine periods in mozambique 2012 2017 |
| topic | rotavirus group A G1P[8] whole genome sequencing Rotarix<sup>®</sup> Bayesian analysis Mozambique |
| url | https://www.mdpi.com/2076-0817/9/12/1026 |
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