Spatiotemporal endometrial transcriptome analysis revealed the luminal epithelium as key player during initial maternal recognition of pregnancy in the mare
Abstract During the period of maternal recognition of pregnancy (MRP) in the mare, the embryo needs to signal its presence to the endometrium to prevent regression of the corpus luteum and prepare for establishment of pregnancy. This is achieved by mechanical stimuli and release of various signaling...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-11-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-01785-3 |
| _version_ | 1819006534784385024 |
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| author | Alba Rudolf Vegas Giorgia Podico Igor F. Canisso Heinrich Bollwein Carmen Almiñana Stefan Bauersachs |
| author_facet | Alba Rudolf Vegas Giorgia Podico Igor F. Canisso Heinrich Bollwein Carmen Almiñana Stefan Bauersachs |
| author_sort | Alba Rudolf Vegas |
| collection | DOAJ |
| description | Abstract During the period of maternal recognition of pregnancy (MRP) in the mare, the embryo needs to signal its presence to the endometrium to prevent regression of the corpus luteum and prepare for establishment of pregnancy. This is achieved by mechanical stimuli and release of various signaling molecules by the equine embryo while migrating through the uterus. We hypothesized that embryo’s signals induce changes in the endometrial gene expression in a highly cell type-specific manner. A spatiotemporal transcriptomics approach was applied combining laser capture microdissection and low-input-RNA sequencing of luminal and glandular epithelium (LE, GE), and stroma of biopsy samples collected from days 10–13 of pregnancy and the estrous cycle. Two comparisons were performed, samples derived from pregnancies with conceptuses ≥ 8 mm in diameter (comparison 1) and conceptuses ≤ 8 mm (comparison 2) versus samples from cyclic controls. The majority of gene expression changes was identified in LE and much lower numbers of differentially expressed genes (DEGs) in GE and stroma. While 1253 DEGs were found for LE in comparison 1, only 248 were found in comparison 2. Data mining mainly focused on DEGs in LE and revealed regulation of genes related to prostaglandin transport, metabolism, and signaling, as well as transcription factor families that could be involved in MRP. In comparison to other mammalian species, differences in regulation of genes involved in epithelial barrier formation and conceptus attachment and implantation reflected the unique features of equine reproduction at the time of MRP at the molecular level. |
| first_indexed | 2024-12-21T00:10:13Z |
| format | Article |
| id | doaj.art-dd31ada8ad2d4b6ca3f941d1b2404799 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-21T00:10:13Z |
| publishDate | 2021-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-dd31ada8ad2d4b6ca3f941d1b24047992022-12-21T19:22:22ZengNature PortfolioScientific Reports2045-23222021-11-0111111710.1038/s41598-021-01785-3Spatiotemporal endometrial transcriptome analysis revealed the luminal epithelium as key player during initial maternal recognition of pregnancy in the mareAlba Rudolf Vegas0Giorgia Podico1Igor F. Canisso2Heinrich Bollwein3Carmen Almiñana4Stefan Bauersachs5Institute of Veterinary Anatomy and Clinic of Reproductive Medicine, Department for Farm Animals, Vetsuisse Faculty Zurich, University of ZurichDepartment of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois Urbana ChampaignDepartment of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois Urbana ChampaignClinic of Reproductive Medicine, Department for Farm Animals, Vetsuisse Faculty Zurich, University of ZurichInstitute of Veterinary Anatomy, Vetsuisse Faculty, University of ZurichInstitute of Veterinary Anatomy, Vetsuisse Faculty, University of ZurichAbstract During the period of maternal recognition of pregnancy (MRP) in the mare, the embryo needs to signal its presence to the endometrium to prevent regression of the corpus luteum and prepare for establishment of pregnancy. This is achieved by mechanical stimuli and release of various signaling molecules by the equine embryo while migrating through the uterus. We hypothesized that embryo’s signals induce changes in the endometrial gene expression in a highly cell type-specific manner. A spatiotemporal transcriptomics approach was applied combining laser capture microdissection and low-input-RNA sequencing of luminal and glandular epithelium (LE, GE), and stroma of biopsy samples collected from days 10–13 of pregnancy and the estrous cycle. Two comparisons were performed, samples derived from pregnancies with conceptuses ≥ 8 mm in diameter (comparison 1) and conceptuses ≤ 8 mm (comparison 2) versus samples from cyclic controls. The majority of gene expression changes was identified in LE and much lower numbers of differentially expressed genes (DEGs) in GE and stroma. While 1253 DEGs were found for LE in comparison 1, only 248 were found in comparison 2. Data mining mainly focused on DEGs in LE and revealed regulation of genes related to prostaglandin transport, metabolism, and signaling, as well as transcription factor families that could be involved in MRP. In comparison to other mammalian species, differences in regulation of genes involved in epithelial barrier formation and conceptus attachment and implantation reflected the unique features of equine reproduction at the time of MRP at the molecular level.https://doi.org/10.1038/s41598-021-01785-3 |
| spellingShingle | Alba Rudolf Vegas Giorgia Podico Igor F. Canisso Heinrich Bollwein Carmen Almiñana Stefan Bauersachs Spatiotemporal endometrial transcriptome analysis revealed the luminal epithelium as key player during initial maternal recognition of pregnancy in the mare Scientific Reports |
| title | Spatiotemporal endometrial transcriptome analysis revealed the luminal epithelium as key player during initial maternal recognition of pregnancy in the mare |
| title_full | Spatiotemporal endometrial transcriptome analysis revealed the luminal epithelium as key player during initial maternal recognition of pregnancy in the mare |
| title_fullStr | Spatiotemporal endometrial transcriptome analysis revealed the luminal epithelium as key player during initial maternal recognition of pregnancy in the mare |
| title_full_unstemmed | Spatiotemporal endometrial transcriptome analysis revealed the luminal epithelium as key player during initial maternal recognition of pregnancy in the mare |
| title_short | Spatiotemporal endometrial transcriptome analysis revealed the luminal epithelium as key player during initial maternal recognition of pregnancy in the mare |
| title_sort | spatiotemporal endometrial transcriptome analysis revealed the luminal epithelium as key player during initial maternal recognition of pregnancy in the mare |
| url | https://doi.org/10.1038/s41598-021-01785-3 |
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