Abnormal Promoter Methylation of Nucleotide-Binding Oligomerization Domain Containing 2 (NOD2) Gene in the Pathogenesis of Crohn’s Disease
Changes in the expression of nucleotide-binding oligomerization domain containing 2 (NOD2) play an important role in the pathogenesis of several autoimmune diseases, such as inflammatory bowel diseases (IBD), including Crohn’s disease (CD) and ulcerative colitis (UC). It seems that epigenetic modif...
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Language: | English |
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Tehran University of Medical Sciences
2023-08-01
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Series: | Acta Medica Iranica |
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Online Access: | https://acta.tums.ac.ir/index.php/acta/article/view/9867 |
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author | Golshid Sanati Mehrdad Noruzinia Davood Jafari Mohammad Ahmadvand Shahram Teimourian Naser Ebrahimi Daryani Nima Rezaei |
author_facet | Golshid Sanati Mehrdad Noruzinia Davood Jafari Mohammad Ahmadvand Shahram Teimourian Naser Ebrahimi Daryani Nima Rezaei |
author_sort | Golshid Sanati |
collection | DOAJ |
description |
Changes in the expression of nucleotide-binding oligomerization domain containing 2 (NOD2) play an important role in the pathogenesis of several autoimmune diseases, such as inflammatory bowel diseases (IBD), including Crohn’s disease (CD) and ulcerative colitis (UC). It seems that epigenetic modifications, including DNA methylation, have an important role in the suppression of gene activity. In this study, the relationship between DNA methylation patterns of the promoter region of the NOD2 gene and the pathogenesis of CD was assessed. Colonic mucosal samples were obtained from 15 Iranian patients with CD and 15 matched healthy controls with no history of autoimmune diseases. After bisulfite conversion of genomic DNA, the DNA methylation status of three CpG sites in the promoter region of the NOD2 gene was determined by the real-time quantitative multiplex methylation-specific PCR assay. Using this approach, we identified a decreased level of methylation of the NOD2 promoter in the colonic mucosa of patients with CD (0.128±0.093 vs. 0.025±0.016, unmethylated DNA in CD vs. healthy controls, respectively, P<0.000). According to our findings, promoter hypomethylation of the NOD2 gene in the colonic mucosa might contribute to the development and severity of CD.
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first_indexed | 2024-03-12T12:24:09Z |
format | Article |
id | doaj.art-dd31ecc92f804074a176fe80620cc0e0 |
institution | Directory Open Access Journal |
issn | 0044-6025 1735-9694 |
language | English |
last_indexed | 2024-03-12T12:24:09Z |
publishDate | 2023-08-01 |
publisher | Tehran University of Medical Sciences |
record_format | Article |
series | Acta Medica Iranica |
spelling | doaj.art-dd31ecc92f804074a176fe80620cc0e02023-08-30T04:27:48ZengTehran University of Medical SciencesActa Medica Iranica0044-60251735-96942023-08-0161510.18502/acta.v61i5.13480Abnormal Promoter Methylation of Nucleotide-Binding Oligomerization Domain Containing 2 (NOD2) Gene in the Pathogenesis of Crohn’s DiseaseGolshid Sanati0Mehrdad Noruzinia1Davood Jafari2Mohammad Ahmadvand3Shahram Teimourian4Naser Ebrahimi Daryani5Nima Rezaei6Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. AND Division of Biomedical Sciences, School of Medicine, University of California, Riverside, USA 3 Network of Immunity in Infection, Malignancy and Autoimmunity (NIIMA), Universal Scientific Education and Research Network (USERN), Riverside, CA, USA.Department of Medical Genetics, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran.Department of Immunology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.Department of Medical Genetics, School of Medical Sciences, Tarbiat Modares University, Tehran, IranDepartment of Genetics, School of Medicine, Iran University of Medical Sciences, Tehran, IranDepartment of Internal Medicine, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran, IranDepartment of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. ANDResearch Center for Immunodeficiencies, Children's Medical Center Hospital, Tehran University of Medical Sciences, Tehran, Iran. AND Network of Immunity in Infection, Malignancy and Autoimmunity (NIIMA), Universal Scientific Education and Research Network (USERN), Tehran, Iran. Changes in the expression of nucleotide-binding oligomerization domain containing 2 (NOD2) play an important role in the pathogenesis of several autoimmune diseases, such as inflammatory bowel diseases (IBD), including Crohn’s disease (CD) and ulcerative colitis (UC). It seems that epigenetic modifications, including DNA methylation, have an important role in the suppression of gene activity. In this study, the relationship between DNA methylation patterns of the promoter region of the NOD2 gene and the pathogenesis of CD was assessed. Colonic mucosal samples were obtained from 15 Iranian patients with CD and 15 matched healthy controls with no history of autoimmune diseases. After bisulfite conversion of genomic DNA, the DNA methylation status of three CpG sites in the promoter region of the NOD2 gene was determined by the real-time quantitative multiplex methylation-specific PCR assay. Using this approach, we identified a decreased level of methylation of the NOD2 promoter in the colonic mucosa of patients with CD (0.128±0.093 vs. 0.025±0.016, unmethylated DNA in CD vs. healthy controls, respectively, P<0.000). According to our findings, promoter hypomethylation of the NOD2 gene in the colonic mucosa might contribute to the development and severity of CD. https://acta.tums.ac.ir/index.php/acta/article/view/9867Nucleotide-binding oligomerization domain containing 2 (NOD2)Inflammatory bowel diseaseCrohn’s diseaseDNA methylation |
spellingShingle | Golshid Sanati Mehrdad Noruzinia Davood Jafari Mohammad Ahmadvand Shahram Teimourian Naser Ebrahimi Daryani Nima Rezaei Abnormal Promoter Methylation of Nucleotide-Binding Oligomerization Domain Containing 2 (NOD2) Gene in the Pathogenesis of Crohn’s Disease Acta Medica Iranica Nucleotide-binding oligomerization domain containing 2 (NOD2) Inflammatory bowel disease Crohn’s disease DNA methylation |
title | Abnormal Promoter Methylation of Nucleotide-Binding Oligomerization Domain Containing 2 (NOD2) Gene in the Pathogenesis of Crohn’s Disease |
title_full | Abnormal Promoter Methylation of Nucleotide-Binding Oligomerization Domain Containing 2 (NOD2) Gene in the Pathogenesis of Crohn’s Disease |
title_fullStr | Abnormal Promoter Methylation of Nucleotide-Binding Oligomerization Domain Containing 2 (NOD2) Gene in the Pathogenesis of Crohn’s Disease |
title_full_unstemmed | Abnormal Promoter Methylation of Nucleotide-Binding Oligomerization Domain Containing 2 (NOD2) Gene in the Pathogenesis of Crohn’s Disease |
title_short | Abnormal Promoter Methylation of Nucleotide-Binding Oligomerization Domain Containing 2 (NOD2) Gene in the Pathogenesis of Crohn’s Disease |
title_sort | abnormal promoter methylation of nucleotide binding oligomerization domain containing 2 nod2 gene in the pathogenesis of crohn s disease |
topic | Nucleotide-binding oligomerization domain containing 2 (NOD2) Inflammatory bowel disease Crohn’s disease DNA methylation |
url | https://acta.tums.ac.ir/index.php/acta/article/view/9867 |
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