Free DNA partially clarifies discrepancies between qPCR and the conventional phage quantification method.

To use phages in a personalized therapy and industrial applications, an accurate quantification is needed. The gold standard method, namely the culture-based double agar overlay (DAO) method, provides an accurate estimate of the number of infectious phages but is laborious and time-intensive. Quanti...

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Podrobná bibliografie
Hlavní autoři: Saar Van Overfelt, Hans Duyvejonck, Femke Baeke, Riet De Rycke, Maya Merabishvili, Stefan Vermeulen, Piet Cools, Mario Vaneechoutte, Els Van Mechelen
Médium: Článek
Jazyk:English
Vydáno: Public Library of Science (PLoS) 2024-01-01
Edice:PLoS ONE
On-line přístup:https://doi.org/10.1371/journal.pone.0313774