EVALUATION OF ANTIOXIDANT ACTIVITY OF IN VITRO PROPAGATED MEDICINAL CERATOPHYLLUM DEMERSUM L. EXTRACTS

Ceratophyllum demersum L. is a medical plant of the family Ceratophyllaceae that has been traditionally used for the treatment of diseases such as ulcer, diarrhoea, wounds and fever since ancient times. This study presents antioxidant activities of water and methanol extracts of in vitro propagated...

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Main Authors: Bugrahan Emsen, Muhammet Dogan
Format: Article
Language:English
Published: University of Life Sciences in Lublin - Publishing House 2019-02-01
Series:Acta Scientiarum Polonorum: Hortorum Cultus
Subjects:
Online Access:https://czasopisma.up.lublin.pl/index.php/asphc/article/view/370
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author Bugrahan Emsen
Muhammet Dogan
author_facet Bugrahan Emsen
Muhammet Dogan
author_sort Bugrahan Emsen
collection DOAJ
description Ceratophyllum demersum L. is a medical plant of the family Ceratophyllaceae that has been traditionally used for the treatment of diseases such as ulcer, diarrhoea, wounds and fever since ancient times. This study presents antioxidant activities of water and methanol extracts of in vitro propagated C. demersum. Shoot tip explants of C. demersum for in vitro plant regeneration were cultured on liquid Murashige and Skoog (MS) medium containing 0.25–1.25 mg/L 6-Benzylaminopurine (BAP) and 0.10 mg/L Thidiazuron (TDZ) combinations for eight weeks. Maximum number of plantlets per explant (110.67) was obtained on MS medium supplemented with 0.75 mg/L BAP + 0.10 mg/L TDZ. In order to determine antioxidant activities of C. demersum, antioxidant contents (β-carotene, flavonoid, lycopene and total phenols), metal chelating and reducing power capacities of methanol and water extracts obtained from C. demersum were investigated. It was detected that water extract was more effective in all activities. Total phenols (76.55 μg/mg) in water extract were measured as the highest antioxidant component. Based on the water extracts data, as IC50 value for chelating on ferrous ions was 9.24 mg/mL, EC50 value for reducing power activity was 8.23 mg/mL. It was also determined that the metal chelating and reducing power activities of the both extracts are increased depending on the concentration. Therefore, all these data suggest that both tested extracts of in vitro propagated C. demersum would exert beneficial effects by virtue of their antioxidant properties and may be utilized as a promising source of therapeutics.
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spelling doaj.art-dd365a67335b418594f93970158572cc2023-12-12T07:55:47ZengUniversity of Life Sciences in Lublin - Publishing HouseActa Scientiarum Polonorum: Hortorum Cultus1644-06922545-14052019-02-0117110.24326/asphc.2018.1.3EVALUATION OF ANTIOXIDANT ACTIVITY OF IN VITRO PROPAGATED MEDICINAL CERATOPHYLLUM DEMERSUM L. EXTRACTSBugrahan Emsen0Muhammet Dogan1Department of Biology, Kamil Özdağ Faculty of Science, Karamanoğlu Mehmetbey University, Karaman, TurkeyDepartment of Biology, Kamil Özdağ Faculty of Science, Karamanoğlu Mehmetbey University, Karaman, Turkey Ceratophyllum demersum L. is a medical plant of the family Ceratophyllaceae that has been traditionally used for the treatment of diseases such as ulcer, diarrhoea, wounds and fever since ancient times. This study presents antioxidant activities of water and methanol extracts of in vitro propagated C. demersum. Shoot tip explants of C. demersum for in vitro plant regeneration were cultured on liquid Murashige and Skoog (MS) medium containing 0.25–1.25 mg/L 6-Benzylaminopurine (BAP) and 0.10 mg/L Thidiazuron (TDZ) combinations for eight weeks. Maximum number of plantlets per explant (110.67) was obtained on MS medium supplemented with 0.75 mg/L BAP + 0.10 mg/L TDZ. In order to determine antioxidant activities of C. demersum, antioxidant contents (β-carotene, flavonoid, lycopene and total phenols), metal chelating and reducing power capacities of methanol and water extracts obtained from C. demersum were investigated. It was detected that water extract was more effective in all activities. Total phenols (76.55 μg/mg) in water extract were measured as the highest antioxidant component. Based on the water extracts data, as IC50 value for chelating on ferrous ions was 9.24 mg/mL, EC50 value for reducing power activity was 8.23 mg/mL. It was also determined that the metal chelating and reducing power activities of the both extracts are increased depending on the concentration. Therefore, all these data suggest that both tested extracts of in vitro propagated C. demersum would exert beneficial effects by virtue of their antioxidant properties and may be utilized as a promising source of therapeutics. https://czasopisma.up.lublin.pl/index.php/asphc/article/view/370shoot regenerationantioxidant componentmetal chelatingreducing power
spellingShingle Bugrahan Emsen
Muhammet Dogan
EVALUATION OF ANTIOXIDANT ACTIVITY OF IN VITRO PROPAGATED MEDICINAL CERATOPHYLLUM DEMERSUM L. EXTRACTS
Acta Scientiarum Polonorum: Hortorum Cultus
shoot regeneration
antioxidant component
metal chelating
reducing power
title EVALUATION OF ANTIOXIDANT ACTIVITY OF IN VITRO PROPAGATED MEDICINAL CERATOPHYLLUM DEMERSUM L. EXTRACTS
title_full EVALUATION OF ANTIOXIDANT ACTIVITY OF IN VITRO PROPAGATED MEDICINAL CERATOPHYLLUM DEMERSUM L. EXTRACTS
title_fullStr EVALUATION OF ANTIOXIDANT ACTIVITY OF IN VITRO PROPAGATED MEDICINAL CERATOPHYLLUM DEMERSUM L. EXTRACTS
title_full_unstemmed EVALUATION OF ANTIOXIDANT ACTIVITY OF IN VITRO PROPAGATED MEDICINAL CERATOPHYLLUM DEMERSUM L. EXTRACTS
title_short EVALUATION OF ANTIOXIDANT ACTIVITY OF IN VITRO PROPAGATED MEDICINAL CERATOPHYLLUM DEMERSUM L. EXTRACTS
title_sort evaluation of antioxidant activity of in vitro propagated medicinal ceratophyllum demersum l extracts
topic shoot regeneration
antioxidant component
metal chelating
reducing power
url https://czasopisma.up.lublin.pl/index.php/asphc/article/view/370
work_keys_str_mv AT bugrahanemsen evaluationofantioxidantactivityofinvitropropagatedmedicinalceratophyllumdemersumlextracts
AT muhammetdogan evaluationofantioxidantactivityofinvitropropagatedmedicinalceratophyllumdemersumlextracts