EVALUATION OF ANTIOXIDANT ACTIVITY OF IN VITRO PROPAGATED MEDICINAL CERATOPHYLLUM DEMERSUM L. EXTRACTS
Ceratophyllum demersum L. is a medical plant of the family Ceratophyllaceae that has been traditionally used for the treatment of diseases such as ulcer, diarrhoea, wounds and fever since ancient times. This study presents antioxidant activities of water and methanol extracts of in vitro propagated...
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University of Life Sciences in Lublin - Publishing House
2019-02-01
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Series: | Acta Scientiarum Polonorum: Hortorum Cultus |
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Online Access: | https://czasopisma.up.lublin.pl/index.php/asphc/article/view/370 |
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author | Bugrahan Emsen Muhammet Dogan |
author_facet | Bugrahan Emsen Muhammet Dogan |
author_sort | Bugrahan Emsen |
collection | DOAJ |
description |
Ceratophyllum demersum L. is a medical plant of the family Ceratophyllaceae that has been traditionally used for the treatment of diseases such as ulcer, diarrhoea, wounds and fever since ancient times. This study presents antioxidant activities of water and methanol extracts of in vitro propagated C. demersum. Shoot tip explants of C. demersum for in vitro plant regeneration were cultured on liquid Murashige and Skoog (MS) medium containing 0.25–1.25 mg/L 6-Benzylaminopurine (BAP) and 0.10 mg/L Thidiazuron (TDZ) combinations for eight weeks. Maximum number of plantlets per explant (110.67) was obtained on MS medium supplemented with 0.75 mg/L BAP + 0.10 mg/L TDZ. In order to determine antioxidant activities of C. demersum, antioxidant contents (β-carotene, flavonoid, lycopene and total phenols), metal chelating and reducing power capacities of methanol and water extracts obtained from C. demersum were investigated. It was detected that water extract was more effective in all activities. Total phenols (76.55 μg/mg) in water extract were measured as the highest antioxidant component. Based on the water extracts data, as IC50 value for chelating on ferrous ions was 9.24 mg/mL, EC50 value for reducing power activity was 8.23 mg/mL. It was also determined that the metal chelating and reducing power activities of the both extracts are increased depending on the concentration. Therefore, all these data suggest that both tested extracts of in vitro propagated C. demersum would exert beneficial effects by virtue of their antioxidant properties and may be utilized as a promising source of therapeutics.
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first_indexed | 2024-03-09T00:16:40Z |
format | Article |
id | doaj.art-dd365a67335b418594f93970158572cc |
institution | Directory Open Access Journal |
issn | 1644-0692 2545-1405 |
language | English |
last_indexed | 2024-03-09T00:16:40Z |
publishDate | 2019-02-01 |
publisher | University of Life Sciences in Lublin - Publishing House |
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series | Acta Scientiarum Polonorum: Hortorum Cultus |
spelling | doaj.art-dd365a67335b418594f93970158572cc2023-12-12T07:55:47ZengUniversity of Life Sciences in Lublin - Publishing HouseActa Scientiarum Polonorum: Hortorum Cultus1644-06922545-14052019-02-0117110.24326/asphc.2018.1.3EVALUATION OF ANTIOXIDANT ACTIVITY OF IN VITRO PROPAGATED MEDICINAL CERATOPHYLLUM DEMERSUM L. EXTRACTSBugrahan Emsen0Muhammet Dogan1Department of Biology, Kamil Özdağ Faculty of Science, Karamanoğlu Mehmetbey University, Karaman, TurkeyDepartment of Biology, Kamil Özdağ Faculty of Science, Karamanoğlu Mehmetbey University, Karaman, Turkey Ceratophyllum demersum L. is a medical plant of the family Ceratophyllaceae that has been traditionally used for the treatment of diseases such as ulcer, diarrhoea, wounds and fever since ancient times. This study presents antioxidant activities of water and methanol extracts of in vitro propagated C. demersum. Shoot tip explants of C. demersum for in vitro plant regeneration were cultured on liquid Murashige and Skoog (MS) medium containing 0.25–1.25 mg/L 6-Benzylaminopurine (BAP) and 0.10 mg/L Thidiazuron (TDZ) combinations for eight weeks. Maximum number of plantlets per explant (110.67) was obtained on MS medium supplemented with 0.75 mg/L BAP + 0.10 mg/L TDZ. In order to determine antioxidant activities of C. demersum, antioxidant contents (β-carotene, flavonoid, lycopene and total phenols), metal chelating and reducing power capacities of methanol and water extracts obtained from C. demersum were investigated. It was detected that water extract was more effective in all activities. Total phenols (76.55 μg/mg) in water extract were measured as the highest antioxidant component. Based on the water extracts data, as IC50 value for chelating on ferrous ions was 9.24 mg/mL, EC50 value for reducing power activity was 8.23 mg/mL. It was also determined that the metal chelating and reducing power activities of the both extracts are increased depending on the concentration. Therefore, all these data suggest that both tested extracts of in vitro propagated C. demersum would exert beneficial effects by virtue of their antioxidant properties and may be utilized as a promising source of therapeutics. https://czasopisma.up.lublin.pl/index.php/asphc/article/view/370shoot regenerationantioxidant componentmetal chelatingreducing power |
spellingShingle | Bugrahan Emsen Muhammet Dogan EVALUATION OF ANTIOXIDANT ACTIVITY OF IN VITRO PROPAGATED MEDICINAL CERATOPHYLLUM DEMERSUM L. EXTRACTS Acta Scientiarum Polonorum: Hortorum Cultus shoot regeneration antioxidant component metal chelating reducing power |
title | EVALUATION OF ANTIOXIDANT ACTIVITY OF IN VITRO PROPAGATED MEDICINAL CERATOPHYLLUM DEMERSUM L. EXTRACTS |
title_full | EVALUATION OF ANTIOXIDANT ACTIVITY OF IN VITRO PROPAGATED MEDICINAL CERATOPHYLLUM DEMERSUM L. EXTRACTS |
title_fullStr | EVALUATION OF ANTIOXIDANT ACTIVITY OF IN VITRO PROPAGATED MEDICINAL CERATOPHYLLUM DEMERSUM L. EXTRACTS |
title_full_unstemmed | EVALUATION OF ANTIOXIDANT ACTIVITY OF IN VITRO PROPAGATED MEDICINAL CERATOPHYLLUM DEMERSUM L. EXTRACTS |
title_short | EVALUATION OF ANTIOXIDANT ACTIVITY OF IN VITRO PROPAGATED MEDICINAL CERATOPHYLLUM DEMERSUM L. EXTRACTS |
title_sort | evaluation of antioxidant activity of in vitro propagated medicinal ceratophyllum demersum l extracts |
topic | shoot regeneration antioxidant component metal chelating reducing power |
url | https://czasopisma.up.lublin.pl/index.php/asphc/article/view/370 |
work_keys_str_mv | AT bugrahanemsen evaluationofantioxidantactivityofinvitropropagatedmedicinalceratophyllumdemersumlextracts AT muhammetdogan evaluationofantioxidantactivityofinvitropropagatedmedicinalceratophyllumdemersumlextracts |