Molecular Cloning and Functional Characterization of Galectin-1 in Yellow Drum (<i>Nibea albiflora</i>)
Galectins are proteins that are involved in the innate immune response against pathogenic microorganisms. In the present study, the gene expression pattern of <i>galectin-1</i> (named as <i>NaGal-1</i>) and its function in mediating the defense response to bacterial attack we...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-02-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/4/3298 |
| Summary: | Galectins are proteins that are involved in the innate immune response against pathogenic microorganisms. In the present study, the gene expression pattern of <i>galectin-1</i> (named as <i>NaGal-1</i>) and its function in mediating the defense response to bacterial attack were investigated. The tertiary structure of <i>Na</i>Gal-1 protein consists of homodimers and each subunit has one carbohydrate recognition domain. Quantitative RT-PCR analysis indicated that <i>NaGal-1</i> was ubiquitously distributed in all the detected tissues and highly expressed in the swim-bladder of <i>Nibea albiflora</i>, and its expression could be upregulated by the pathogenic <i>Vibrio harveyi</i> attack in the brain. Expression of <i>Na</i>Gal-1 protein in HEK 293T cells was distributed in the cytoplasm as well as in the nucleus. The recombinant <i>Na</i>Gal-1 protein by prokaryotic expression could agglutinate red blood cells from rabbit, <i>Larimichthys crocea</i>, and <i>N. albiflora</i>. The agglutination of <i>N. albiflora</i> red blood cells by the recombinant <i>Na</i>Gal-1 protein was inhibited by peptidoglycan, lactose, D-galactose, and lipopolysaccharide in certain concentrations. In addition, the recombinant <i>Na</i>Gal-1 protein agglutinated and killed some gram-negative bacteria including <i>Edwardsiella tarda</i>, <i>Escherichia coli</i>, <i>Photobacterium phosphoreum</i>, <i>Aeromonas hydrophila</i>, <i>Pseudomonas aeruginosa</i>, and <i>Aeromonas veronii</i>. These results set the stage for further studies of <i>Na</i>Gal-1 protein in the innate immunity of <i>N. albiflora</i>. |
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| ISSN: | 1661-6596 1422-0067 |