Molecular Cloning and Functional Characterization of Galectin-1 in Yellow Drum (<i>Nibea albiflora</i>)
Galectins are proteins that are involved in the innate immune response against pathogenic microorganisms. In the present study, the gene expression pattern of <i>galectin-1</i> (named as <i>NaGal-1</i>) and its function in mediating the defense response to bacterial attack we...
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MDPI AG
2023-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/4/3298 |
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| author | Baolan Wu Qiaoying Li Wanbo Li Shuai Luo Fang Han Zhiyong Wang |
| author_facet | Baolan Wu Qiaoying Li Wanbo Li Shuai Luo Fang Han Zhiyong Wang |
| author_sort | Baolan Wu |
| collection | DOAJ |
| description | Galectins are proteins that are involved in the innate immune response against pathogenic microorganisms. In the present study, the gene expression pattern of <i>galectin-1</i> (named as <i>NaGal-1</i>) and its function in mediating the defense response to bacterial attack were investigated. The tertiary structure of <i>Na</i>Gal-1 protein consists of homodimers and each subunit has one carbohydrate recognition domain. Quantitative RT-PCR analysis indicated that <i>NaGal-1</i> was ubiquitously distributed in all the detected tissues and highly expressed in the swim-bladder of <i>Nibea albiflora</i>, and its expression could be upregulated by the pathogenic <i>Vibrio harveyi</i> attack in the brain. Expression of <i>Na</i>Gal-1 protein in HEK 293T cells was distributed in the cytoplasm as well as in the nucleus. The recombinant <i>Na</i>Gal-1 protein by prokaryotic expression could agglutinate red blood cells from rabbit, <i>Larimichthys crocea</i>, and <i>N. albiflora</i>. The agglutination of <i>N. albiflora</i> red blood cells by the recombinant <i>Na</i>Gal-1 protein was inhibited by peptidoglycan, lactose, D-galactose, and lipopolysaccharide in certain concentrations. In addition, the recombinant <i>Na</i>Gal-1 protein agglutinated and killed some gram-negative bacteria including <i>Edwardsiella tarda</i>, <i>Escherichia coli</i>, <i>Photobacterium phosphoreum</i>, <i>Aeromonas hydrophila</i>, <i>Pseudomonas aeruginosa</i>, and <i>Aeromonas veronii</i>. These results set the stage for further studies of <i>Na</i>Gal-1 protein in the innate immunity of <i>N. albiflora</i>. |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T08:44:26Z |
| publishDate | 2023-02-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-dd37450fcfa04c1493a546e0945f1d662023-11-16T20:57:59ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-02-01244329810.3390/ijms24043298Molecular Cloning and Functional Characterization of Galectin-1 in Yellow Drum (<i>Nibea albiflora</i>)Baolan Wu0Qiaoying Li1Wanbo Li2Shuai Luo3Fang Han4Zhiyong Wang5Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural Affairs, Fisheries College, Jimei University, Xiamen 361021, ChinaKey Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural Affairs, Fisheries College, Jimei University, Xiamen 361021, ChinaKey Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural Affairs, Fisheries College, Jimei University, Xiamen 361021, ChinaKey Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural Affairs, Fisheries College, Jimei University, Xiamen 361021, ChinaKey Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural Affairs, Fisheries College, Jimei University, Xiamen 361021, ChinaKey Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural Affairs, Fisheries College, Jimei University, Xiamen 361021, ChinaGalectins are proteins that are involved in the innate immune response against pathogenic microorganisms. In the present study, the gene expression pattern of <i>galectin-1</i> (named as <i>NaGal-1</i>) and its function in mediating the defense response to bacterial attack were investigated. The tertiary structure of <i>Na</i>Gal-1 protein consists of homodimers and each subunit has one carbohydrate recognition domain. Quantitative RT-PCR analysis indicated that <i>NaGal-1</i> was ubiquitously distributed in all the detected tissues and highly expressed in the swim-bladder of <i>Nibea albiflora</i>, and its expression could be upregulated by the pathogenic <i>Vibrio harveyi</i> attack in the brain. Expression of <i>Na</i>Gal-1 protein in HEK 293T cells was distributed in the cytoplasm as well as in the nucleus. The recombinant <i>Na</i>Gal-1 protein by prokaryotic expression could agglutinate red blood cells from rabbit, <i>Larimichthys crocea</i>, and <i>N. albiflora</i>. The agglutination of <i>N. albiflora</i> red blood cells by the recombinant <i>Na</i>Gal-1 protein was inhibited by peptidoglycan, lactose, D-galactose, and lipopolysaccharide in certain concentrations. In addition, the recombinant <i>Na</i>Gal-1 protein agglutinated and killed some gram-negative bacteria including <i>Edwardsiella tarda</i>, <i>Escherichia coli</i>, <i>Photobacterium phosphoreum</i>, <i>Aeromonas hydrophila</i>, <i>Pseudomonas aeruginosa</i>, and <i>Aeromonas veronii</i>. These results set the stage for further studies of <i>Na</i>Gal-1 protein in the innate immunity of <i>N. albiflora</i>.https://www.mdpi.com/1422-0067/24/4/3298galectin-1agglutinationantibacterial activityinnate immunity<i>Nibea albiflora</i> |
| spellingShingle | Baolan Wu Qiaoying Li Wanbo Li Shuai Luo Fang Han Zhiyong Wang Molecular Cloning and Functional Characterization of Galectin-1 in Yellow Drum (<i>Nibea albiflora</i>) International Journal of Molecular Sciences galectin-1 agglutination antibacterial activity innate immunity <i>Nibea albiflora</i> |
| title | Molecular Cloning and Functional Characterization of Galectin-1 in Yellow Drum (<i>Nibea albiflora</i>) |
| title_full | Molecular Cloning and Functional Characterization of Galectin-1 in Yellow Drum (<i>Nibea albiflora</i>) |
| title_fullStr | Molecular Cloning and Functional Characterization of Galectin-1 in Yellow Drum (<i>Nibea albiflora</i>) |
| title_full_unstemmed | Molecular Cloning and Functional Characterization of Galectin-1 in Yellow Drum (<i>Nibea albiflora</i>) |
| title_short | Molecular Cloning and Functional Characterization of Galectin-1 in Yellow Drum (<i>Nibea albiflora</i>) |
| title_sort | molecular cloning and functional characterization of galectin 1 in yellow drum i nibea albiflora i |
| topic | galectin-1 agglutination antibacterial activity innate immunity <i>Nibea albiflora</i> |
| url | https://www.mdpi.com/1422-0067/24/4/3298 |
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