Dual Transcriptomic Analysis Reveals a Delayed Antiviral Response of <i>Haliotis diversicolor supertexta</i> against Haliotid Herpesvirus-1
Haliotid herpesvirus-1 (HaHV-1) is the first identified gastropod herpesvirus, causing a highly lethal neurologic disease of abalone species. The genome of HaHV-1 has been sequenced, but the functions of the putative genes and their roles during infection are still poorly understood. In the present...
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MDPI AG
2019-04-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/11/4/383 |
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| author | Chang-Ming Bai Shu-Min Zhang Ya-Na Li Lu-Sheng Xin Umberto Rosani Chong-Ming Wang |
| author_facet | Chang-Ming Bai Shu-Min Zhang Ya-Na Li Lu-Sheng Xin Umberto Rosani Chong-Ming Wang |
| author_sort | Chang-Ming Bai |
| collection | DOAJ |
| description | Haliotid herpesvirus-1 (HaHV-1) is the first identified gastropod herpesvirus, causing a highly lethal neurologic disease of abalone species. The genome of HaHV-1 has been sequenced, but the functions of the putative genes and their roles during infection are still poorly understood. In the present study, transcriptomic profiles of <i>Haliotis diversicolor supertexta</i> at 0, 24 and 60 h post injection (hpi) with HaHV-1 were characterized through high-throughput RNA sequencing. A total of 448 M raw reads were obtained and assembled into 2.08 × 10<sup>5</sup> unigenes with a mean length of 1486 bp and an N50 of 2455 bp. Although we detected increased HaHV-1 DNA loads and active viral expression at 24 hpi, this evidence was not linked to significant changes of host transcriptomic profiles between 0 and 24 hpi, whereas a rich immune-related gene set was over-expressed at 60 hpi. These results indicate that, at least at the beginning of HaHV-1 infection, the virus can replicate with no activation of the host immune response. We propose that HaHV-1 may evolve more effective strategies to modulate the host immune response and hide during replication, so that it could evade the immune surveillance at the early stage of infection. |
| first_indexed | 2024-12-14T03:53:21Z |
| format | Article |
| id | doaj.art-dd3794e2e73a44e68145de6427629582 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-12-14T03:53:21Z |
| publishDate | 2019-04-01 |
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| record_format | Article |
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| spelling | doaj.art-dd3794e2e73a44e68145de64276295822022-12-21T23:18:09ZengMDPI AGViruses1999-49152019-04-0111438310.3390/v11040383v11040383Dual Transcriptomic Analysis Reveals a Delayed Antiviral Response of <i>Haliotis diversicolor supertexta</i> against Haliotid Herpesvirus-1Chang-Ming Bai0Shu-Min Zhang1Ya-Na Li2Lu-Sheng Xin3Umberto Rosani4Chong-Ming Wang5Key Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture, Qingdao Key Laboratory of Mariculture Epidemiology and Biosecurity, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, ChinaKey Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture, Qingdao Key Laboratory of Mariculture Epidemiology and Biosecurity, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, ChinaKey Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture, Qingdao Key Laboratory of Mariculture Epidemiology and Biosecurity, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, ChinaKey Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture, Qingdao Key Laboratory of Mariculture Epidemiology and Biosecurity, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, ChinaDepartment of Biology, University of Padua, 35121 Padua, ItalyKey Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture, Qingdao Key Laboratory of Mariculture Epidemiology and Biosecurity, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, ChinaHaliotid herpesvirus-1 (HaHV-1) is the first identified gastropod herpesvirus, causing a highly lethal neurologic disease of abalone species. The genome of HaHV-1 has been sequenced, but the functions of the putative genes and their roles during infection are still poorly understood. In the present study, transcriptomic profiles of <i>Haliotis diversicolor supertexta</i> at 0, 24 and 60 h post injection (hpi) with HaHV-1 were characterized through high-throughput RNA sequencing. A total of 448 M raw reads were obtained and assembled into 2.08 × 10<sup>5</sup> unigenes with a mean length of 1486 bp and an N50 of 2455 bp. Although we detected increased HaHV-1 DNA loads and active viral expression at 24 hpi, this evidence was not linked to significant changes of host transcriptomic profiles between 0 and 24 hpi, whereas a rich immune-related gene set was over-expressed at 60 hpi. These results indicate that, at least at the beginning of HaHV-1 infection, the virus can replicate with no activation of the host immune response. We propose that HaHV-1 may evolve more effective strategies to modulate the host immune response and hide during replication, so that it could evade the immune surveillance at the early stage of infection.https://www.mdpi.com/1999-4915/11/4/383abalonetranscriptomeimmune-related genesapoptosisHaHV-1 |
| spellingShingle | Chang-Ming Bai Shu-Min Zhang Ya-Na Li Lu-Sheng Xin Umberto Rosani Chong-Ming Wang Dual Transcriptomic Analysis Reveals a Delayed Antiviral Response of <i>Haliotis diversicolor supertexta</i> against Haliotid Herpesvirus-1 Viruses abalone transcriptome immune-related genes apoptosis HaHV-1 |
| title | Dual Transcriptomic Analysis Reveals a Delayed Antiviral Response of <i>Haliotis diversicolor supertexta</i> against Haliotid Herpesvirus-1 |
| title_full | Dual Transcriptomic Analysis Reveals a Delayed Antiviral Response of <i>Haliotis diversicolor supertexta</i> against Haliotid Herpesvirus-1 |
| title_fullStr | Dual Transcriptomic Analysis Reveals a Delayed Antiviral Response of <i>Haliotis diversicolor supertexta</i> against Haliotid Herpesvirus-1 |
| title_full_unstemmed | Dual Transcriptomic Analysis Reveals a Delayed Antiviral Response of <i>Haliotis diversicolor supertexta</i> against Haliotid Herpesvirus-1 |
| title_short | Dual Transcriptomic Analysis Reveals a Delayed Antiviral Response of <i>Haliotis diversicolor supertexta</i> against Haliotid Herpesvirus-1 |
| title_sort | dual transcriptomic analysis reveals a delayed antiviral response of i haliotis diversicolor supertexta i against haliotid herpesvirus 1 |
| topic | abalone transcriptome immune-related genes apoptosis HaHV-1 |
| url | https://www.mdpi.com/1999-4915/11/4/383 |
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