Immunological parameters and gene polymorphisms (C-590T IL4, C-597A IL10) in severe bronchial asthma in children from the Krasnoyarsk region, West Siberia
Background. Bronchial asthma is a common disease caused by interplay between multiple determinants, including genetic and immune variations. Objective. To investigate the main indices of humoral and cellular branches of immunity, features of cytokine regulation and cytokine genes in children with at...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2013-08-01
|
| Series: | International Journal of Circumpolar Health |
| Subjects: | |
| Online Access: | http://www.circumpolarhealthjournal.net/index.php/ijch/article/download/21159/pdf_1 |
| _version_ | 1818276961845248000 |
|---|---|
| author | Marina V. Smolnikova Svetlana V. Smirnova Maxim B. Freidin Olga S. Tyutina |
| author_facet | Marina V. Smolnikova Svetlana V. Smirnova Maxim B. Freidin Olga S. Tyutina |
| author_sort | Marina V. Smolnikova |
| collection | DOAJ |
| description | Background. Bronchial asthma is a common disease caused by interplay between multiple determinants, including genetic and immune variations. Objective. To investigate the main indices of humoral and cellular branches of immunity, features of cytokine regulation and cytokine genes in children with atopic bronchial asthma (BA) with different levels of disease control. Design. Fifty children with controlled BA (CBA) and 50 with uncontrolled BA (UBA) were analyzed. Mean age in the sample was 13.36±2.24 years. A control group of healthy children (n=50) was also studied. All individuals were Russians (Eastern Slavs) from the Krasnoyarsk Territory, West Siberia. Diagnoses, severity and level of disease control were defined according to the Global Initiative for Asthma (GINA) recommendations. The lymphocytes were counted in blood using fluorescent microscopy. Humoral branch indices and cytokine levels (IL-2, IL-4, IL-10 and TNF-α) in blood serum were measured by ELISA. Genotyping of single-nucleotide polymorphism (SNP) in −590 position of the IL4 and −597 position of the IL10 gene was performed by restriction fragment length analysis. Results. No statistically significant differences in total IgE and cytokines blood levels were found in CBA and UBA. However, significant differences between the groups were found for CD3+, CD4+, and CD8+, cell counts. The T-590 allele of the IL4 gene, which is responsible for an increased serum level of IL-4, showed a tendency to an association with UBA. A decreased level of IL-10 enhances control over BA, which proves its association with the allelic variant A-597 IL10. Conclusions. Our data show that children with UBA have higher counts of CD3+, cells and an increase of sub-population of CD4+, -cells as well as higher levels of IgE, IL-4 and TNF-α in blood serum as compared to CBA. Polymorphisms of the IL4 and IL10 genes are associated with allergic inflammation in UBA. |
| first_indexed | 2024-12-12T22:53:58Z |
| format | Article |
| id | doaj.art-dd3b5944bcd040d38c669b629ce91c9b |
| institution | Directory Open Access Journal |
| issn | 2242-3982 |
| language | English |
| last_indexed | 2024-12-12T22:53:58Z |
| publishDate | 2013-08-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | International Journal of Circumpolar Health |
| spelling | doaj.art-dd3b5944bcd040d38c669b629ce91c9b2022-12-22T00:09:00ZengTaylor & Francis GroupInternational Journal of Circumpolar Health2242-39822013-08-017201710.3402/ijch.v72i0.21159Immunological parameters and gene polymorphisms (C-590T IL4, C-597A IL10) in severe bronchial asthma in children from the Krasnoyarsk region, West SiberiaMarina V. SmolnikovaSvetlana V. SmirnovaMaxim B. FreidinOlga S. TyutinaBackground. Bronchial asthma is a common disease caused by interplay between multiple determinants, including genetic and immune variations. Objective. To investigate the main indices of humoral and cellular branches of immunity, features of cytokine regulation and cytokine genes in children with atopic bronchial asthma (BA) with different levels of disease control. Design. Fifty children with controlled BA (CBA) and 50 with uncontrolled BA (UBA) were analyzed. Mean age in the sample was 13.36±2.24 years. A control group of healthy children (n=50) was also studied. All individuals were Russians (Eastern Slavs) from the Krasnoyarsk Territory, West Siberia. Diagnoses, severity and level of disease control were defined according to the Global Initiative for Asthma (GINA) recommendations. The lymphocytes were counted in blood using fluorescent microscopy. Humoral branch indices and cytokine levels (IL-2, IL-4, IL-10 and TNF-α) in blood serum were measured by ELISA. Genotyping of single-nucleotide polymorphism (SNP) in −590 position of the IL4 and −597 position of the IL10 gene was performed by restriction fragment length analysis. Results. No statistically significant differences in total IgE and cytokines blood levels were found in CBA and UBA. However, significant differences between the groups were found for CD3+, CD4+, and CD8+, cell counts. The T-590 allele of the IL4 gene, which is responsible for an increased serum level of IL-4, showed a tendency to an association with UBA. A decreased level of IL-10 enhances control over BA, which proves its association with the allelic variant A-597 IL10. Conclusions. Our data show that children with UBA have higher counts of CD3+, cells and an increase of sub-population of CD4+, -cells as well as higher levels of IgE, IL-4 and TNF-α in blood serum as compared to CBA. Polymorphisms of the IL4 and IL10 genes are associated with allergic inflammation in UBA.http://www.circumpolarhealthjournal.net/index.php/ijch/article/download/21159/pdf_1asthmacytokinesingle-nucleotide polymorphismIL-4IL-10 |
| spellingShingle | Marina V. Smolnikova Svetlana V. Smirnova Maxim B. Freidin Olga S. Tyutina Immunological parameters and gene polymorphisms (C-590T IL4, C-597A IL10) in severe bronchial asthma in children from the Krasnoyarsk region, West Siberia International Journal of Circumpolar Health asthma cytokine single-nucleotide polymorphism IL-4 IL-10 |
| title | Immunological parameters and gene polymorphisms (C-590T IL4, C-597A IL10) in severe bronchial asthma in children from the Krasnoyarsk region, West Siberia |
| title_full | Immunological parameters and gene polymorphisms (C-590T IL4, C-597A IL10) in severe bronchial asthma in children from the Krasnoyarsk region, West Siberia |
| title_fullStr | Immunological parameters and gene polymorphisms (C-590T IL4, C-597A IL10) in severe bronchial asthma in children from the Krasnoyarsk region, West Siberia |
| title_full_unstemmed | Immunological parameters and gene polymorphisms (C-590T IL4, C-597A IL10) in severe bronchial asthma in children from the Krasnoyarsk region, West Siberia |
| title_short | Immunological parameters and gene polymorphisms (C-590T IL4, C-597A IL10) in severe bronchial asthma in children from the Krasnoyarsk region, West Siberia |
| title_sort | immunological parameters and gene polymorphisms c 590t il4 c 597a il10 in severe bronchial asthma in children from the krasnoyarsk region west siberia |
| topic | asthma cytokine single-nucleotide polymorphism IL-4 IL-10 |
| url | http://www.circumpolarhealthjournal.net/index.php/ijch/article/download/21159/pdf_1 |
| work_keys_str_mv | AT marinavsmolnikova immunologicalparametersandgenepolymorphismsc590til4c597ail10inseverebronchialasthmainchildrenfromthekrasnoyarskregionwestsiberia AT svetlanavsmirnova immunologicalparametersandgenepolymorphismsc590til4c597ail10inseverebronchialasthmainchildrenfromthekrasnoyarskregionwestsiberia AT maximbfreidin immunologicalparametersandgenepolymorphismsc590til4c597ail10inseverebronchialasthmainchildrenfromthekrasnoyarskregionwestsiberia AT olgastyutina immunologicalparametersandgenepolymorphismsc590til4c597ail10inseverebronchialasthmainchildrenfromthekrasnoyarskregionwestsiberia |