Label-free Live and Dead Cell Separation Method Using a High-Efficiency Optically-Induced Dielectrophoretic (ODEP) Force-based Microfluidic Platform
<p>This study reports an optically-induced dielectrophoretic (ODEP) force-based microfluidic platform for live and dead cell separation and collection. ODEP forces are used to separate the live and dead cells due to their opposite responses to an ODEP force. Combining the flow control in a mic...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Chinese Institute of Automation Engineers (CIAE) & Taiwan Smart Living Space Association (SMART LISA)
2014-05-01
|
| Series: | International Journal of Automation and Smart Technology |
| Subjects: | |
| Online Access: | http://www.ausmt.org/index.php/AUSMT/article/view/302 |
| _version_ | 1811255439978921984 |
|---|---|
| author | Song-Bin Huang Shing-Lun Liu Jian-Ting Li Min-Hsien Wu |
| author_facet | Song-Bin Huang Shing-Lun Liu Jian-Ting Li Min-Hsien Wu |
| author_sort | Song-Bin Huang |
| collection | DOAJ |
| description | <p>This study reports an optically-induced dielectrophoretic (ODEP) force-based microfluidic platform for live and dead cell separation and collection. ODEP forces are used to separate the live and dead cells due to their opposite responses to an ODEP force. Combining the flow control in a microfluidic system, the live and dead cells can be separated and subsequently collected in an efficient and effective manner. The operating conditions of the ODEP force for manipulating the live and dead chondrocytes is characterized, and separation performance is experimentally evaluated. Results revealed that an applied voltage of 8 V resulted in a maximum difference of manipulation force for the live (49.4 pN) and dead (-20.1 pN) cells. Results of further separation experiments showed that the recovery rate and purity of the isolated live cells was as high as 78.3±6.8 % and 96.4 ±2.2 %, respectively. Overall, the proposed method is found to be particularly valuable for biological research which requires the isolation of highly pure live or dead cells.</p> |
| first_indexed | 2024-04-12T17:23:58Z |
| format | Article |
| id | doaj.art-dd3e0053a1f74b0e94ba42c7cbdaef53 |
| institution | Directory Open Access Journal |
| issn | 2223-9766 |
| language | English |
| last_indexed | 2024-04-12T17:23:58Z |
| publishDate | 2014-05-01 |
| publisher | Chinese Institute of Automation Engineers (CIAE) & Taiwan Smart Living Space Association (SMART LISA) |
| record_format | Article |
| series | International Journal of Automation and Smart Technology |
| spelling | doaj.art-dd3e0053a1f74b0e94ba42c7cbdaef532022-12-22T03:23:22ZengChinese Institute of Automation Engineers (CIAE) & Taiwan Smart Living Space Association (SMART LISA)International Journal of Automation and Smart Technology2223-97662014-05-0142839110.5875/ausmt.v4i2.302117Label-free Live and Dead Cell Separation Method Using a High-Efficiency Optically-Induced Dielectrophoretic (ODEP) Force-based Microfluidic PlatformSong-Bin Huang0Shing-Lun Liu1Jian-Ting Li2Min-Hsien Wu3Graduate Institute of Biochemical and Biomedical Engineering, Chang Gung UniversityGraduate Institute of Biochemical and Biomedical Engineering, Chang Gung UniversityGraduate Institute of Biochemical and Biomedical Engineering, Chang Gung UniversityGraduate Institute of Biochemical and Biomedical Engineering, Chang Gung University<p>This study reports an optically-induced dielectrophoretic (ODEP) force-based microfluidic platform for live and dead cell separation and collection. ODEP forces are used to separate the live and dead cells due to their opposite responses to an ODEP force. Combining the flow control in a microfluidic system, the live and dead cells can be separated and subsequently collected in an efficient and effective manner. The operating conditions of the ODEP force for manipulating the live and dead chondrocytes is characterized, and separation performance is experimentally evaluated. Results revealed that an applied voltage of 8 V resulted in a maximum difference of manipulation force for the live (49.4 pN) and dead (-20.1 pN) cells. Results of further separation experiments showed that the recovery rate and purity of the isolated live cells was as high as 78.3±6.8 % and 96.4 ±2.2 %, respectively. Overall, the proposed method is found to be particularly valuable for biological research which requires the isolation of highly pure live or dead cells.</p>http://www.ausmt.org/index.php/AUSMT/article/view/302MicrofluidicsOptically induced dielectrophoretic (ODEP) forceCell separationLive and dead cells |
| spellingShingle | Song-Bin Huang Shing-Lun Liu Jian-Ting Li Min-Hsien Wu Label-free Live and Dead Cell Separation Method Using a High-Efficiency Optically-Induced Dielectrophoretic (ODEP) Force-based Microfluidic Platform International Journal of Automation and Smart Technology Microfluidics Optically induced dielectrophoretic (ODEP) force Cell separation Live and dead cells |
| title | Label-free Live and Dead Cell Separation Method Using a High-Efficiency Optically-Induced Dielectrophoretic (ODEP) Force-based Microfluidic Platform |
| title_full | Label-free Live and Dead Cell Separation Method Using a High-Efficiency Optically-Induced Dielectrophoretic (ODEP) Force-based Microfluidic Platform |
| title_fullStr | Label-free Live and Dead Cell Separation Method Using a High-Efficiency Optically-Induced Dielectrophoretic (ODEP) Force-based Microfluidic Platform |
| title_full_unstemmed | Label-free Live and Dead Cell Separation Method Using a High-Efficiency Optically-Induced Dielectrophoretic (ODEP) Force-based Microfluidic Platform |
| title_short | Label-free Live and Dead Cell Separation Method Using a High-Efficiency Optically-Induced Dielectrophoretic (ODEP) Force-based Microfluidic Platform |
| title_sort | label free live and dead cell separation method using a high efficiency optically induced dielectrophoretic odep force based microfluidic platform |
| topic | Microfluidics Optically induced dielectrophoretic (ODEP) force Cell separation Live and dead cells |
| url | http://www.ausmt.org/index.php/AUSMT/article/view/302 |
| work_keys_str_mv | AT songbinhuang labelfreeliveanddeadcellseparationmethodusingahighefficiencyopticallyinduceddielectrophoreticodepforcebasedmicrofluidicplatform AT shinglunliu labelfreeliveanddeadcellseparationmethodusingahighefficiencyopticallyinduceddielectrophoreticodepforcebasedmicrofluidicplatform AT jiantingli labelfreeliveanddeadcellseparationmethodusingahighefficiencyopticallyinduceddielectrophoreticodepforcebasedmicrofluidicplatform AT minhsienwu labelfreeliveanddeadcellseparationmethodusingahighefficiencyopticallyinduceddielectrophoreticodepforcebasedmicrofluidicplatform |