Evidence for the Involvement of the Chemosensory Protein AgosCSP5 in Resistance to Insecticides in the Cotton Aphid, <i>Aphis gossypii</i>
It has been speculated that insect chemosensory proteins (CSPs) may have additional roles beyond olfaction. In this study, the phylogenetic and genomic analyses of the CSPs of the cotton aphid, <i>Aphis gossypii</i>, revealed the presence of gene gain-and-loss among different aphid field...
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MDPI AG
2021-04-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/12/4/335 |
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| author | Fen Li Herbert Venthur Shang Wang Rafael A. Homem Jing-Jiang Zhou |
| author_facet | Fen Li Herbert Venthur Shang Wang Rafael A. Homem Jing-Jiang Zhou |
| author_sort | Fen Li |
| collection | DOAJ |
| description | It has been speculated that insect chemosensory proteins (CSPs) may have additional roles beyond olfaction. In this study, the phylogenetic and genomic analyses of the CSPs of the cotton aphid, <i>Aphis gossypii</i>, revealed the presence of gene gain-and-loss among different aphid field populations. Differential expressions of eight <i>CSP</i> genes were demonstrated after treatments with insecticides of different modes of action. The expression of <i>AgosCSP5</i> was significantly upregulated by the insecticide treatments in a dose-dependent manner. The <i>Drosophila</i> flies overexpressing <i>AgosCSP5</i> were significantly less susceptible to the insecticides, omethoate, imidacloprid and cypermethrin but not to deltamethrin and tau-fluvalinate, compared with control flies. The transgenic <i>Drosophila</i> flies exhibited an LC<sub>50</sub> resistance ratio of 2.6 to omethoate, compared with control flies. Likewise, the mortality of the transgenic flies to imidacloprid and cypermethrin was significantly lower than that of the control flies (<i>p</i> < 0.01). Homology modelling, molecular docking and dynamic simulation supported the interactions and revealed a higher stability of AgosCSP5/insecticide complexes than AgosCSP5/semiochemical complexes. Our study demonstrates for first time the in vivo evidence for the involvement of <i>CSP</i> genes in insecticide resistance of crop insect pests and provides new insights of the newly discovered CSP-mediated insect resistance mechanism to insecticides. |
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| last_indexed | 2024-03-10T12:29:22Z |
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| spelling | doaj.art-dd40f9116bee47dc992f6c320d2cbebf2023-11-21T14:47:21ZengMDPI AGInsects2075-44502021-04-0112433510.3390/insects12040335Evidence for the Involvement of the Chemosensory Protein AgosCSP5 in Resistance to Insecticides in the Cotton Aphid, <i>Aphis gossypii</i>Fen Li0Herbert Venthur1Shang Wang2Rafael A. Homem3Jing-Jiang Zhou4Department of Plant Protection, Hainan University, Haikou 570228, ChinaLaboratorio de Química Ecológica, Departamento de Ciencias Químicas y Recursos Naturales, Centro de Investigación Biotecnológica, Aplicada al Medio Ambiente (CIBAMA), Universidad de La Frontera, Temuco 54-D, ChileCollege of Plant Sciences, Jilin University, Changchun 130062, ChinaDepartment of Biointeractions and Crop Protection, Rothamsted Research, Harpenden AL5 2JQ, UKBiocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, Gansu Agricultural University, No. 1 Yingmen Village, Anning District, Lanzhou 730070, ChinaIt has been speculated that insect chemosensory proteins (CSPs) may have additional roles beyond olfaction. In this study, the phylogenetic and genomic analyses of the CSPs of the cotton aphid, <i>Aphis gossypii</i>, revealed the presence of gene gain-and-loss among different aphid field populations. Differential expressions of eight <i>CSP</i> genes were demonstrated after treatments with insecticides of different modes of action. The expression of <i>AgosCSP5</i> was significantly upregulated by the insecticide treatments in a dose-dependent manner. The <i>Drosophila</i> flies overexpressing <i>AgosCSP5</i> were significantly less susceptible to the insecticides, omethoate, imidacloprid and cypermethrin but not to deltamethrin and tau-fluvalinate, compared with control flies. The transgenic <i>Drosophila</i> flies exhibited an LC<sub>50</sub> resistance ratio of 2.6 to omethoate, compared with control flies. Likewise, the mortality of the transgenic flies to imidacloprid and cypermethrin was significantly lower than that of the control flies (<i>p</i> < 0.01). Homology modelling, molecular docking and dynamic simulation supported the interactions and revealed a higher stability of AgosCSP5/insecticide complexes than AgosCSP5/semiochemical complexes. Our study demonstrates for first time the in vivo evidence for the involvement of <i>CSP</i> genes in insecticide resistance of crop insect pests and provides new insights of the newly discovered CSP-mediated insect resistance mechanism to insecticides.https://www.mdpi.com/2075-4450/12/4/335chemosensory proteinpesticide resistanceinsect pest management |
| spellingShingle | Fen Li Herbert Venthur Shang Wang Rafael A. Homem Jing-Jiang Zhou Evidence for the Involvement of the Chemosensory Protein AgosCSP5 in Resistance to Insecticides in the Cotton Aphid, <i>Aphis gossypii</i> Insects chemosensory protein pesticide resistance insect pest management |
| title | Evidence for the Involvement of the Chemosensory Protein AgosCSP5 in Resistance to Insecticides in the Cotton Aphid, <i>Aphis gossypii</i> |
| title_full | Evidence for the Involvement of the Chemosensory Protein AgosCSP5 in Resistance to Insecticides in the Cotton Aphid, <i>Aphis gossypii</i> |
| title_fullStr | Evidence for the Involvement of the Chemosensory Protein AgosCSP5 in Resistance to Insecticides in the Cotton Aphid, <i>Aphis gossypii</i> |
| title_full_unstemmed | Evidence for the Involvement of the Chemosensory Protein AgosCSP5 in Resistance to Insecticides in the Cotton Aphid, <i>Aphis gossypii</i> |
| title_short | Evidence for the Involvement of the Chemosensory Protein AgosCSP5 in Resistance to Insecticides in the Cotton Aphid, <i>Aphis gossypii</i> |
| title_sort | evidence for the involvement of the chemosensory protein agoscsp5 in resistance to insecticides in the cotton aphid i aphis gossypii i |
| topic | chemosensory protein pesticide resistance insect pest management |
| url | https://www.mdpi.com/2075-4450/12/4/335 |
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