Visualization of Protein Aggregation in Nerve Cells After Ischemia/Reperfusion by Ubiquitin Immunohistochemistry and Impregnative Nauta Method
Using ubiquitin immunohistochemistry and impregnative Nauta method we demonstrated that ubiquitin positivity and Nauta positivity in the neurons affected with ischemic injury in the lumbosacral spinal cord of rabbits and dogs may be of the same origin. Increased number of ubiquitin-positive aggregat...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Karolinum Press
2004-01-01
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| Series: | Acta Medica |
| Subjects: | |
| Online Access: | https://actamedica.lfhk.cuni.cz/47/3/0209/ |
| _version_ | 1811337734277562368 |
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| author | Eva Mechírová Iveta Domoráková Marianna Feriková |
| author_facet | Eva Mechírová Iveta Domoráková Marianna Feriková |
| author_sort | Eva Mechírová |
| collection | DOAJ |
| description | Using ubiquitin immunohistochemistry and impregnative Nauta method we demonstrated that ubiquitin positivity and Nauta positivity in the neurons affected with ischemic injury in the lumbosacral spinal cord of rabbits and dogs may be of the same origin. Increased number of ubiquitin-positive aggregates was found in the cytoplasm of neurons in the intermediate zone and lamina IX of ventral horns of spinal cord in rabbits after 30 min of ischemia followed by 24 h lasting reperfusion. Nauta-positive, flocculent, intracytoplasmic, dark clusters appeared in the same localization in the canine lumbosacral spinal cord neurons after 30 min of ischemia and 24 h of reperfusion. Ubiquitin aggregates and Nauta-positive dark clusters in the injured spinal cord neurons could be the first light microscopic signs of slow neuronal death following spinal cord ischemia and reperfusion. |
| first_indexed | 2024-04-13T17:59:12Z |
| format | Article |
| id | doaj.art-dd419e6cdfe74af4a439ea38840dc785 |
| institution | Directory Open Access Journal |
| issn | 1211-4286 1805-9694 |
| language | English |
| last_indexed | 2024-04-13T17:59:12Z |
| publishDate | 2004-01-01 |
| publisher | Karolinum Press |
| record_format | Article |
| series | Acta Medica |
| spelling | doaj.art-dd419e6cdfe74af4a439ea38840dc7852022-12-22T02:36:20ZengKarolinum PressActa Medica1211-42861805-96942004-01-0147320921110.14712/18059694.2018.94Visualization of Protein Aggregation in Nerve Cells After Ischemia/Reperfusion by Ubiquitin Immunohistochemistry and Impregnative Nauta MethodEva Mechírová0Iveta Domoráková1Marianna Feriková2P.J. Šafárik University, Faculty of Medicine, Department of Histology and Embryology, Košice, Slovak RepublicP.J. Šafárik University, Faculty of Medicine, Department of Histology and Embryology, Košice, Slovak RepublicP.J. Šafárik University, Faculty of Medicine, Department of Histology and Embryology, Košice, Slovak RepublicUsing ubiquitin immunohistochemistry and impregnative Nauta method we demonstrated that ubiquitin positivity and Nauta positivity in the neurons affected with ischemic injury in the lumbosacral spinal cord of rabbits and dogs may be of the same origin. Increased number of ubiquitin-positive aggregates was found in the cytoplasm of neurons in the intermediate zone and lamina IX of ventral horns of spinal cord in rabbits after 30 min of ischemia followed by 24 h lasting reperfusion. Nauta-positive, flocculent, intracytoplasmic, dark clusters appeared in the same localization in the canine lumbosacral spinal cord neurons after 30 min of ischemia and 24 h of reperfusion. Ubiquitin aggregates and Nauta-positive dark clusters in the injured spinal cord neurons could be the first light microscopic signs of slow neuronal death following spinal cord ischemia and reperfusion.https://actamedica.lfhk.cuni.cz/47/3/0209/Spinal cordRabbitDogIschemiaUbiquitinImmunoreactivity |
| spellingShingle | Eva Mechírová Iveta Domoráková Marianna Feriková Visualization of Protein Aggregation in Nerve Cells After Ischemia/Reperfusion by Ubiquitin Immunohistochemistry and Impregnative Nauta Method Acta Medica Spinal cord Rabbit Dog Ischemia Ubiquitin Immunoreactivity |
| title | Visualization of Protein Aggregation in Nerve Cells After Ischemia/Reperfusion by Ubiquitin Immunohistochemistry and Impregnative Nauta Method |
| title_full | Visualization of Protein Aggregation in Nerve Cells After Ischemia/Reperfusion by Ubiquitin Immunohistochemistry and Impregnative Nauta Method |
| title_fullStr | Visualization of Protein Aggregation in Nerve Cells After Ischemia/Reperfusion by Ubiquitin Immunohistochemistry and Impregnative Nauta Method |
| title_full_unstemmed | Visualization of Protein Aggregation in Nerve Cells After Ischemia/Reperfusion by Ubiquitin Immunohistochemistry and Impregnative Nauta Method |
| title_short | Visualization of Protein Aggregation in Nerve Cells After Ischemia/Reperfusion by Ubiquitin Immunohistochemistry and Impregnative Nauta Method |
| title_sort | visualization of protein aggregation in nerve cells after ischemia reperfusion by ubiquitin immunohistochemistry and impregnative nauta method |
| topic | Spinal cord Rabbit Dog Ischemia Ubiquitin Immunoreactivity |
| url | https://actamedica.lfhk.cuni.cz/47/3/0209/ |
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