Red Wine Extract Inhibits VEGF Secretion and Its Signaling Pathway in Retinal ARPE-19 Cells to Potentially Disrupt AMD
Age-related macular degeneration (AMD) is a degenerative disease of the retina where the molecular mechanism involves the production of vascular endothelial growth factor (VEGF), a factor of poor prognosis of the progression of the disease. Previous studies have shown that resveratrol, a polyphenol...
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| Format: | Article |
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MDPI AG
2020-11-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/23/5564 |
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| author | Clarisse Cornebise Flavie Courtaut Marie Taillandier-Coindard Josep Valls-Fonayet Tristan Richard David Monchaud Virginie Aires Dominique Delmas |
| author_facet | Clarisse Cornebise Flavie Courtaut Marie Taillandier-Coindard Josep Valls-Fonayet Tristan Richard David Monchaud Virginie Aires Dominique Delmas |
| author_sort | Clarisse Cornebise |
| collection | DOAJ |
| description | Age-related macular degeneration (AMD) is a degenerative disease of the retina where the molecular mechanism involves the production of vascular endothelial growth factor (VEGF), a factor of poor prognosis of the progression of the disease. Previous studies have shown that resveratrol, a polyphenol of grapevines, can prevent VEGF secretion induced by stress from retinal cells. Considering the fundamental role played by VEGF in development and progression of AMD, we investigate the potential effect of red wine extract (RWE) on VEGF secretion and its signaling pathway in human retinal cells ARPE-19. To examine the effect of RWE in ARPE-19, a quantitative and qualitative analysis of the RWE was performed by HPLC MS/MS. We show for the first time that RWE decreased VEGF-A secretion from ARPE-19 cells and its protein expression in concentration-dependent manner. RWE-induced alteration in VEGF-A production is associated with a down of VEGF-receptor 2 (VEGF-R2) protein expression and its phosphorylated intracytoplasmic domain. Subsequently, the activation of kinase pathway is disturbing and RWE prevents the phosphorylation of MEK and ERK 1/2 in human retinal cells ARPE-19. Finally, this study sheds light on the interest that the use of polyphenolic cocktails could represent in a prevention strategy. |
| first_indexed | 2024-03-10T14:31:28Z |
| format | Article |
| id | doaj.art-dd425f2c33c7461b89205488b3e75cf0 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T14:31:28Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-dd425f2c33c7461b89205488b3e75cf02023-11-20T22:32:50ZengMDPI AGMolecules1420-30492020-11-012523556410.3390/molecules25235564Red Wine Extract Inhibits VEGF Secretion and Its Signaling Pathway in Retinal ARPE-19 Cells to Potentially Disrupt AMDClarisse Cornebise0Flavie Courtaut1Marie Taillandier-Coindard2Josep Valls-Fonayet3Tristan Richard4David Monchaud5Virginie Aires6Dominique Delmas7Université de Bourgogne Franche-Comté, F-21000 Dijon, FranceUniversité de Bourgogne Franche-Comté, F-21000 Dijon, FranceUniversité de Bourgogne Franche-Comté, F-21000 Dijon, FranceUnité de Recherche Oenologie, EA 4577, USC 1366 INRA-ISVV, F-33882 Villenave d’Ornon, FranceUnité de Recherche Oenologie, EA 4577, USC 1366 INRA-ISVV, F-33882 Villenave d’Ornon, FranceUniversité de Bourgogne Franche-Comté, F-21000 Dijon, FranceUniversité de Bourgogne Franche-Comté, F-21000 Dijon, FranceUniversité de Bourgogne Franche-Comté, F-21000 Dijon, FranceAge-related macular degeneration (AMD) is a degenerative disease of the retina where the molecular mechanism involves the production of vascular endothelial growth factor (VEGF), a factor of poor prognosis of the progression of the disease. Previous studies have shown that resveratrol, a polyphenol of grapevines, can prevent VEGF secretion induced by stress from retinal cells. Considering the fundamental role played by VEGF in development and progression of AMD, we investigate the potential effect of red wine extract (RWE) on VEGF secretion and its signaling pathway in human retinal cells ARPE-19. To examine the effect of RWE in ARPE-19, a quantitative and qualitative analysis of the RWE was performed by HPLC MS/MS. We show for the first time that RWE decreased VEGF-A secretion from ARPE-19 cells and its protein expression in concentration-dependent manner. RWE-induced alteration in VEGF-A production is associated with a down of VEGF-receptor 2 (VEGF-R2) protein expression and its phosphorylated intracytoplasmic domain. Subsequently, the activation of kinase pathway is disturbing and RWE prevents the phosphorylation of MEK and ERK 1/2 in human retinal cells ARPE-19. Finally, this study sheds light on the interest that the use of polyphenolic cocktails could represent in a prevention strategy.https://www.mdpi.com/1420-3049/25/23/5564polyphenolsred wine extractAMDretinal cellsARPE-19degenerative diseases |
| spellingShingle | Clarisse Cornebise Flavie Courtaut Marie Taillandier-Coindard Josep Valls-Fonayet Tristan Richard David Monchaud Virginie Aires Dominique Delmas Red Wine Extract Inhibits VEGF Secretion and Its Signaling Pathway in Retinal ARPE-19 Cells to Potentially Disrupt AMD Molecules polyphenols red wine extract AMD retinal cells ARPE-19 degenerative diseases |
| title | Red Wine Extract Inhibits VEGF Secretion and Its Signaling Pathway in Retinal ARPE-19 Cells to Potentially Disrupt AMD |
| title_full | Red Wine Extract Inhibits VEGF Secretion and Its Signaling Pathway in Retinal ARPE-19 Cells to Potentially Disrupt AMD |
| title_fullStr | Red Wine Extract Inhibits VEGF Secretion and Its Signaling Pathway in Retinal ARPE-19 Cells to Potentially Disrupt AMD |
| title_full_unstemmed | Red Wine Extract Inhibits VEGF Secretion and Its Signaling Pathway in Retinal ARPE-19 Cells to Potentially Disrupt AMD |
| title_short | Red Wine Extract Inhibits VEGF Secretion and Its Signaling Pathway in Retinal ARPE-19 Cells to Potentially Disrupt AMD |
| title_sort | red wine extract inhibits vegf secretion and its signaling pathway in retinal arpe 19 cells to potentially disrupt amd |
| topic | polyphenols red wine extract AMD retinal cells ARPE-19 degenerative diseases |
| url | https://www.mdpi.com/1420-3049/25/23/5564 |
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