Impact of Visceral Leishmaniasis on Local Organ Metabolism in Hamsters
<i>Leishmania</i> is an intracellular parasite with different species pathogenic to humans and causing the disease leishmaniasis. <i>Leishmania donovani</i> causes visceral leishmaniasis (VL) that manifests as hepatosplenomegaly, fever, pancytopenia and hypergammaglobulinemia...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-08-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/12/9/802 |
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| author | Mahbobeh Lesani Camil Gosmanov Andrea Paun Michael D. Lewis Laura-Isobel McCall |
| author_facet | Mahbobeh Lesani Camil Gosmanov Andrea Paun Michael D. Lewis Laura-Isobel McCall |
| author_sort | Mahbobeh Lesani |
| collection | DOAJ |
| description | <i>Leishmania</i> is an intracellular parasite with different species pathogenic to humans and causing the disease leishmaniasis. <i>Leishmania donovani</i> causes visceral leishmaniasis (VL) that manifests as hepatosplenomegaly, fever, pancytopenia and hypergammaglobulinemia. If left without treatment, VL can cause death, especially in immunocompromised people. Current treatments have often significant adverse effects, and resistance has been reported in some countries. Determining the metabolites perturbed during VL can lead us to find new treatments targeting disease pathogenesis. We therefore compared metabolic perturbation between <i>L. donovani</i>-infected and uninfected hamsters across organs (spleen, liver, and gut). Metabolites were extracted, analyzed by liquid chromatography-mass spectrometry, and processed with MZmine and molecular networking to annotate metabolites. We found few metabolites commonly impacted by infection across all three sites, including glycerophospholipids, ceramides, acylcarnitines, peptides, purines and amino acids. In accordance with VL symptoms and parasite tropism, we found a greater overlap of perturbed metabolites between spleen and liver compared to spleen and gut, or liver and gut. Targeting pathways related to these metabolite families would be the next focus that can lead us to find more effective treatments for VL. |
| first_indexed | 2024-03-09T23:12:12Z |
| format | Article |
| id | doaj.art-dd428336682a4fcaa36ce9bd4eb94fca |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-09T23:12:12Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-dd428336682a4fcaa36ce9bd4eb94fca2023-11-23T17:43:52ZengMDPI AGMetabolites2218-19892022-08-0112980210.3390/metabo12090802Impact of Visceral Leishmaniasis on Local Organ Metabolism in HamstersMahbobeh Lesani0Camil Gosmanov1Andrea Paun2Michael D. Lewis3Laura-Isobel McCall4Department of Microbiology and Plant Biology, University of Oklahoma, Norman, OK 73019, USADepartment of Chemistry and Biochemistry, University of Oklahoma, Norman, OK 73019, USALaboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, NIH, Bethesda, MD 20892, USADepartment of Infection Biology, London School of Hygiene and Tropical Medicine, London WC1E 7HT, UKDepartment of Microbiology and Plant Biology, University of Oklahoma, Norman, OK 73019, USA<i>Leishmania</i> is an intracellular parasite with different species pathogenic to humans and causing the disease leishmaniasis. <i>Leishmania donovani</i> causes visceral leishmaniasis (VL) that manifests as hepatosplenomegaly, fever, pancytopenia and hypergammaglobulinemia. If left without treatment, VL can cause death, especially in immunocompromised people. Current treatments have often significant adverse effects, and resistance has been reported in some countries. Determining the metabolites perturbed during VL can lead us to find new treatments targeting disease pathogenesis. We therefore compared metabolic perturbation between <i>L. donovani</i>-infected and uninfected hamsters across organs (spleen, liver, and gut). Metabolites were extracted, analyzed by liquid chromatography-mass spectrometry, and processed with MZmine and molecular networking to annotate metabolites. We found few metabolites commonly impacted by infection across all three sites, including glycerophospholipids, ceramides, acylcarnitines, peptides, purines and amino acids. In accordance with VL symptoms and parasite tropism, we found a greater overlap of perturbed metabolites between spleen and liver compared to spleen and gut, or liver and gut. Targeting pathways related to these metabolite families would be the next focus that can lead us to find more effective treatments for VL.https://www.mdpi.com/2218-1989/12/9/802visceral leishmaniasis<i>Leishmania donovani</i>metabolitesliverspleengut |
| spellingShingle | Mahbobeh Lesani Camil Gosmanov Andrea Paun Michael D. Lewis Laura-Isobel McCall Impact of Visceral Leishmaniasis on Local Organ Metabolism in Hamsters Metabolites visceral leishmaniasis <i>Leishmania donovani</i> metabolites liver spleen gut |
| title | Impact of Visceral Leishmaniasis on Local Organ Metabolism in Hamsters |
| title_full | Impact of Visceral Leishmaniasis on Local Organ Metabolism in Hamsters |
| title_fullStr | Impact of Visceral Leishmaniasis on Local Organ Metabolism in Hamsters |
| title_full_unstemmed | Impact of Visceral Leishmaniasis on Local Organ Metabolism in Hamsters |
| title_short | Impact of Visceral Leishmaniasis on Local Organ Metabolism in Hamsters |
| title_sort | impact of visceral leishmaniasis on local organ metabolism in hamsters |
| topic | visceral leishmaniasis <i>Leishmania donovani</i> metabolites liver spleen gut |
| url | https://www.mdpi.com/2218-1989/12/9/802 |
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