Label free detection of vitamin D by microcantilever-based aptasensor
Vitamin D deficiency or insufficiency is a growing public health problem. In this work, a novel and sample approach based on microcantilever for measuring the level of 25-hydroxyvitamin D (25(OH)D) was proposed. The Bruno aptamer was utilized to functionalize the microcantilevers, serving as a sensi...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-12-01
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| Series: | Journal of King Saud University: Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1018364723004135 |
| _version_ | 1797616026561544192 |
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| author | Khalid E. Alzahrani Mahmoud Al-Gawati Abdulaziz K. Assaifan Abdullah Alodhayb Khalid Alotaibi Abdullah Alswieleh Hamad Albrithen Amal F. Alanazi |
| author_facet | Khalid E. Alzahrani Mahmoud Al-Gawati Abdulaziz K. Assaifan Abdullah Alodhayb Khalid Alotaibi Abdullah Alswieleh Hamad Albrithen Amal F. Alanazi |
| author_sort | Khalid E. Alzahrani |
| collection | DOAJ |
| description | Vitamin D deficiency or insufficiency is a growing public health problem. In this work, a novel and sample approach based on microcantilever for measuring the level of 25-hydroxyvitamin D (25(OH)D) was proposed. The Bruno aptamer was utilized to functionalize the microcantilevers, serving as a sensing layer for detecting 25(OH)D. The working principle of the sensor is based on the changes of the microcantilever deflection and frequency as a result of the aptamer removal from the surface of the microcantilever resulting from its interaction with vitamin D. The results showed a linear dependence between vitamin D concentration and the microcantilever deflection and frequency shift in a range of concentrations from 1 to 300 nM. For this biosensor, the limit of detection (LOD) was calculated to be ∼ 0.3 nM. The biosensor selectivity was tested against vitamin C and the sensor showed high selectivity towards vitamin D. |
| first_indexed | 2024-03-11T07:34:21Z |
| format | Article |
| id | doaj.art-dd434a3217c346ab9c15cb36ce1970fa |
| institution | Directory Open Access Journal |
| issn | 1018-3647 |
| language | English |
| last_indexed | 2024-03-11T07:34:21Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of King Saud University: Science |
| spelling | doaj.art-dd434a3217c346ab9c15cb36ce1970fa2023-11-17T05:25:19ZengElsevierJournal of King Saud University: Science1018-36472023-12-013510102951Label free detection of vitamin D by microcantilever-based aptasensorKhalid E. Alzahrani0Mahmoud Al-Gawati1Abdulaziz K. Assaifan2Abdullah Alodhayb3Khalid Alotaibi4Abdullah Alswieleh5Hamad Albrithen6Amal F. Alanazi7Department of Physics and Astronomy, College of Science, King Saud University, Riyadh 11451, Saudi Arabia; Corresponding author.Department of Physics and Astronomy, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Biomedical Technology, College of Applied Medical Sciences, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Physics and Astronomy, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Chemistry, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Chemistry, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Physics and Astronomy, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaEnergy, Water and Environment Lab, College of Humanities and Sciences, Prince Sultan University, Riyadh 11586, Saudi ArabiaVitamin D deficiency or insufficiency is a growing public health problem. In this work, a novel and sample approach based on microcantilever for measuring the level of 25-hydroxyvitamin D (25(OH)D) was proposed. The Bruno aptamer was utilized to functionalize the microcantilevers, serving as a sensing layer for detecting 25(OH)D. The working principle of the sensor is based on the changes of the microcantilever deflection and frequency as a result of the aptamer removal from the surface of the microcantilever resulting from its interaction with vitamin D. The results showed a linear dependence between vitamin D concentration and the microcantilever deflection and frequency shift in a range of concentrations from 1 to 300 nM. For this biosensor, the limit of detection (LOD) was calculated to be ∼ 0.3 nM. The biosensor selectivity was tested against vitamin C and the sensor showed high selectivity towards vitamin D.http://www.sciencedirect.com/science/article/pii/S1018364723004135MicrocantileverAptamerVitamin D25-hydroxyvitaminAptasensor |
| spellingShingle | Khalid E. Alzahrani Mahmoud Al-Gawati Abdulaziz K. Assaifan Abdullah Alodhayb Khalid Alotaibi Abdullah Alswieleh Hamad Albrithen Amal F. Alanazi Label free detection of vitamin D by microcantilever-based aptasensor Journal of King Saud University: Science Microcantilever Aptamer Vitamin D 25-hydroxyvitamin Aptasensor |
| title | Label free detection of vitamin D by microcantilever-based aptasensor |
| title_full | Label free detection of vitamin D by microcantilever-based aptasensor |
| title_fullStr | Label free detection of vitamin D by microcantilever-based aptasensor |
| title_full_unstemmed | Label free detection of vitamin D by microcantilever-based aptasensor |
| title_short | Label free detection of vitamin D by microcantilever-based aptasensor |
| title_sort | label free detection of vitamin d by microcantilever based aptasensor |
| topic | Microcantilever Aptamer Vitamin D 25-hydroxyvitamin Aptasensor |
| url | http://www.sciencedirect.com/science/article/pii/S1018364723004135 |
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