Caries induced cytokine network in the odontoblast layer of human teeth
<p>Abstract</p> <p>Background</p> <p>Immunologic responses of the tooth to caries begin with odontoblasts recognizing carious bacteria. Inflammatory propagation eventually leads to tooth pulp necrosis and danger to health. The present study aims to determine cytokine ge...
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| Format: | Article |
| Language: | English |
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BMC
2011-01-01
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| Series: | BMC Immunology |
| Online Access: | http://www.biomedcentral.com/1471-2172/12/9 |
| _version_ | 1811265708523257856 |
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| author | Horst Jeremy A Horst Orapin V Samudrala Ram Dale Beverly A |
| author_facet | Horst Jeremy A Horst Orapin V Samudrala Ram Dale Beverly A |
| author_sort | Horst Jeremy A |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Immunologic responses of the tooth to caries begin with odontoblasts recognizing carious bacteria. Inflammatory propagation eventually leads to tooth pulp necrosis and danger to health. The present study aims to determine cytokine gene expression profiles generated within human teeth in response to dental caries <it>in vivo </it>and to build a mechanistic model of these responses and the downstream signaling network.</p> <p>Results</p> <p>We demonstrate profound differential up-regulation of inflammatory genes in the odontoblast layer (ODL) in human teeth with caries <it>in vivo</it>, while the pulp remains largely unchanged. Interleukins, chemokines, and all tested receptors thereof were differentially up-regulated in ODL of carious teeth, well over one hundred-fold for 35 of 84 genes. By interrogating reconstructed protein interaction networks corresponding to the differentially up-regulated genes, we develop the hypothesis that pro-inflammatory cytokines highly expressed in ODL of carious teeth, IL-1β, IL-1α, and TNF-α, carry the converged inflammatory signal. We show that IL1β amplifies antimicrobial peptide production in odontoblasts <it>in vitro </it>100-fold more than lipopolysaccharide, in a manner matching subsequent <it>in vivo </it>measurements.</p> <p>Conclusions</p> <p>Our data suggest that ODL amplifies bacterial signals dramatically by self-feedback cytokine-chemokine signal-receptor cycling, and signal convergence through IL1R1 and possibly others, to increase defensive capacity including antimicrobial peptide production to protect the tooth and contain the battle against carious bacteria within the dentin.</p> |
| first_indexed | 2024-04-12T20:28:50Z |
| format | Article |
| id | doaj.art-dd453ba4b18b4276ba3262bed54129a6 |
| institution | Directory Open Access Journal |
| issn | 1471-2172 |
| language | English |
| last_indexed | 2024-04-12T20:28:50Z |
| publishDate | 2011-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Immunology |
| spelling | doaj.art-dd453ba4b18b4276ba3262bed54129a62022-12-22T03:17:48ZengBMCBMC Immunology1471-21722011-01-01121910.1186/1471-2172-12-9Caries induced cytokine network in the odontoblast layer of human teethHorst Jeremy AHorst Orapin VSamudrala RamDale Beverly A<p>Abstract</p> <p>Background</p> <p>Immunologic responses of the tooth to caries begin with odontoblasts recognizing carious bacteria. Inflammatory propagation eventually leads to tooth pulp necrosis and danger to health. The present study aims to determine cytokine gene expression profiles generated within human teeth in response to dental caries <it>in vivo </it>and to build a mechanistic model of these responses and the downstream signaling network.</p> <p>Results</p> <p>We demonstrate profound differential up-regulation of inflammatory genes in the odontoblast layer (ODL) in human teeth with caries <it>in vivo</it>, while the pulp remains largely unchanged. Interleukins, chemokines, and all tested receptors thereof were differentially up-regulated in ODL of carious teeth, well over one hundred-fold for 35 of 84 genes. By interrogating reconstructed protein interaction networks corresponding to the differentially up-regulated genes, we develop the hypothesis that pro-inflammatory cytokines highly expressed in ODL of carious teeth, IL-1β, IL-1α, and TNF-α, carry the converged inflammatory signal. We show that IL1β amplifies antimicrobial peptide production in odontoblasts <it>in vitro </it>100-fold more than lipopolysaccharide, in a manner matching subsequent <it>in vivo </it>measurements.</p> <p>Conclusions</p> <p>Our data suggest that ODL amplifies bacterial signals dramatically by self-feedback cytokine-chemokine signal-receptor cycling, and signal convergence through IL1R1 and possibly others, to increase defensive capacity including antimicrobial peptide production to protect the tooth and contain the battle against carious bacteria within the dentin.</p>http://www.biomedcentral.com/1471-2172/12/9 |
| spellingShingle | Horst Jeremy A Horst Orapin V Samudrala Ram Dale Beverly A Caries induced cytokine network in the odontoblast layer of human teeth BMC Immunology |
| title | Caries induced cytokine network in the odontoblast layer of human teeth |
| title_full | Caries induced cytokine network in the odontoblast layer of human teeth |
| title_fullStr | Caries induced cytokine network in the odontoblast layer of human teeth |
| title_full_unstemmed | Caries induced cytokine network in the odontoblast layer of human teeth |
| title_short | Caries induced cytokine network in the odontoblast layer of human teeth |
| title_sort | caries induced cytokine network in the odontoblast layer of human teeth |
| url | http://www.biomedcentral.com/1471-2172/12/9 |
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