Obacunone alleviates chronic pelvic pain and pro-inflammatory depolarization of macrophage induced by experimental autoimmune prostatitis in mice
Chronic pelvic pain syndrome (CPPS) is a common complication of prostatitis, which was associated with the pathological depolarization of macrophage and the neuroinflammation. However, its underlying reason is far from clear and few effective treatments is applicable. In this study, we tested the ef...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-12-01
|
| Series: | Biochemistry and Biophysics Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405580823001462 |
| _version_ | 1797422603260919808 |
|---|---|
| author | Yadong Wang Zhaohui Dang Xu Wang Yuanyuan Chen Peng Dong Gang Liu Weibin Tan Zhong Gui Fan Bu Feng Lin Chaozhao Liang |
| author_facet | Yadong Wang Zhaohui Dang Xu Wang Yuanyuan Chen Peng Dong Gang Liu Weibin Tan Zhong Gui Fan Bu Feng Lin Chaozhao Liang |
| author_sort | Yadong Wang |
| collection | DOAJ |
| description | Chronic pelvic pain syndrome (CPPS) is a common complication of prostatitis, which was associated with the pathological depolarization of macrophage and the neuroinflammation. However, its underlying reason is far from clear and few effective treatments is applicable. In this study, we tested the effect of obacunone (Oba), a highly oxygenated triterpenoid, on CPPS. The experimental autoimmune prostatitis (EAP) was induced by subcutaneous injection of heterologous prostate homogenate in mice. We found that EAP led to prostatodynia, neuronal activation of spinal dorsal horn, and the pro-inflammatory depolarization of macrophage within prostate, which was significantly alleviated by oral administration of Oba in a dose-dependent manner. Mechanistically, EAP-induced production of IL-6 on prostatic macrophage was suppressed by Oba. Moreover, co-administration of Oba and MIF inhibitor ISO-1 did not lead to additive effect when compared with either alone. In summary, we conclude that Oba prevents the production of macrophage-derived pro-inflammatory factors by inhibiting MIF, which eventually alleviates CPPS after prostatitis. |
| first_indexed | 2024-03-09T07:34:43Z |
| format | Article |
| id | doaj.art-dd4564ce17a9496c858098424daaf635 |
| institution | Directory Open Access Journal |
| issn | 2405-5808 |
| language | English |
| last_indexed | 2024-03-09T07:34:43Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biochemistry and Biophysics Reports |
| spelling | doaj.art-dd4564ce17a9496c858098424daaf6352023-12-03T05:41:56ZengElsevierBiochemistry and Biophysics Reports2405-58082023-12-0136101565Obacunone alleviates chronic pelvic pain and pro-inflammatory depolarization of macrophage induced by experimental autoimmune prostatitis in miceYadong Wang0Zhaohui Dang1Xu Wang2Yuanyuan Chen3Peng Dong4Gang Liu5Weibin Tan6Zhong Gui7Fan Bu8Feng Lin9Chaozhao Liang10Department of Urology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, 230022, China; Department of Urology, The Fourth Clinical Medical College of Guangzhou University of Chinese Medicine, Shenzhen, Guangdong, 518033, ChinaDepartment of Neurology & Psychology, The Fourth Clinical Medical College of Guangzhou University of Chinese Medicine, Shenzhen, Guangdong, 518033, ChinaDepartment of Neurology & Psychology, The Fourth Clinical Medical College of Guangzhou University of Chinese Medicine, Shenzhen, Guangdong, 518033, ChinaDepartment of Neurology & Psychology, The Fourth Clinical Medical College of Guangzhou University of Chinese Medicine, Shenzhen, Guangdong, 518033, ChinaDepartment of Neurosurgery, The Fourth Clinical Medical College of Guangzhou University of Chinese Medicine, Shenzhen, Guangdong, 518033, ChinaDepartment of Neurosurgery, The Fourth Clinical Medical College of Guangzhou University of Chinese Medicine, Shenzhen, Guangdong, 518033, ChinaDepartment of Neurosurgery, The Fourth Clinical Medical College of Guangzhou University of Chinese Medicine, Shenzhen, Guangdong, 518033, ChinaDepartment of Neurosurgery, The Fourth Clinical Medical College of Guangzhou University of Chinese Medicine, Shenzhen, Guangdong, 518033, ChinaDepartment of Neurology & Psychology, The Fourth Clinical Medical College of Guangzhou University of Chinese Medicine, Shenzhen, Guangdong, 518033, China; Corresponding author.Department of Urology, The Fourth Clinical Medical College of Guangzhou University of Chinese Medicine, Shenzhen, Guangdong, 518033, China; Corresponding author.Department of Urology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, 230022, China; Corresponding author.Chronic pelvic pain syndrome (CPPS) is a common complication of prostatitis, which was associated with the pathological depolarization of macrophage and the neuroinflammation. However, its underlying reason is far from clear and few effective treatments is applicable. In this study, we tested the effect of obacunone (Oba), a highly oxygenated triterpenoid, on CPPS. The experimental autoimmune prostatitis (EAP) was induced by subcutaneous injection of heterologous prostate homogenate in mice. We found that EAP led to prostatodynia, neuronal activation of spinal dorsal horn, and the pro-inflammatory depolarization of macrophage within prostate, which was significantly alleviated by oral administration of Oba in a dose-dependent manner. Mechanistically, EAP-induced production of IL-6 on prostatic macrophage was suppressed by Oba. Moreover, co-administration of Oba and MIF inhibitor ISO-1 did not lead to additive effect when compared with either alone. In summary, we conclude that Oba prevents the production of macrophage-derived pro-inflammatory factors by inhibiting MIF, which eventually alleviates CPPS after prostatitis.http://www.sciencedirect.com/science/article/pii/S2405580823001462Chronic pelvic pain syndromeExperimental autoimmune prostatitisMacrophageMacrophage migration inhibitory factorObacunone |
| spellingShingle | Yadong Wang Zhaohui Dang Xu Wang Yuanyuan Chen Peng Dong Gang Liu Weibin Tan Zhong Gui Fan Bu Feng Lin Chaozhao Liang Obacunone alleviates chronic pelvic pain and pro-inflammatory depolarization of macrophage induced by experimental autoimmune prostatitis in mice Biochemistry and Biophysics Reports Chronic pelvic pain syndrome Experimental autoimmune prostatitis Macrophage Macrophage migration inhibitory factor Obacunone |
| title | Obacunone alleviates chronic pelvic pain and pro-inflammatory depolarization of macrophage induced by experimental autoimmune prostatitis in mice |
| title_full | Obacunone alleviates chronic pelvic pain and pro-inflammatory depolarization of macrophage induced by experimental autoimmune prostatitis in mice |
| title_fullStr | Obacunone alleviates chronic pelvic pain and pro-inflammatory depolarization of macrophage induced by experimental autoimmune prostatitis in mice |
| title_full_unstemmed | Obacunone alleviates chronic pelvic pain and pro-inflammatory depolarization of macrophage induced by experimental autoimmune prostatitis in mice |
| title_short | Obacunone alleviates chronic pelvic pain and pro-inflammatory depolarization of macrophage induced by experimental autoimmune prostatitis in mice |
| title_sort | obacunone alleviates chronic pelvic pain and pro inflammatory depolarization of macrophage induced by experimental autoimmune prostatitis in mice |
| topic | Chronic pelvic pain syndrome Experimental autoimmune prostatitis Macrophage Macrophage migration inhibitory factor Obacunone |
| url | http://www.sciencedirect.com/science/article/pii/S2405580823001462 |
| work_keys_str_mv | AT yadongwang obacunonealleviateschronicpelvicpainandproinflammatorydepolarizationofmacrophageinducedbyexperimentalautoimmuneprostatitisinmice AT zhaohuidang obacunonealleviateschronicpelvicpainandproinflammatorydepolarizationofmacrophageinducedbyexperimentalautoimmuneprostatitisinmice AT xuwang obacunonealleviateschronicpelvicpainandproinflammatorydepolarizationofmacrophageinducedbyexperimentalautoimmuneprostatitisinmice AT yuanyuanchen obacunonealleviateschronicpelvicpainandproinflammatorydepolarizationofmacrophageinducedbyexperimentalautoimmuneprostatitisinmice AT pengdong obacunonealleviateschronicpelvicpainandproinflammatorydepolarizationofmacrophageinducedbyexperimentalautoimmuneprostatitisinmice AT gangliu obacunonealleviateschronicpelvicpainandproinflammatorydepolarizationofmacrophageinducedbyexperimentalautoimmuneprostatitisinmice AT weibintan obacunonealleviateschronicpelvicpainandproinflammatorydepolarizationofmacrophageinducedbyexperimentalautoimmuneprostatitisinmice AT zhonggui obacunonealleviateschronicpelvicpainandproinflammatorydepolarizationofmacrophageinducedbyexperimentalautoimmuneprostatitisinmice AT fanbu obacunonealleviateschronicpelvicpainandproinflammatorydepolarizationofmacrophageinducedbyexperimentalautoimmuneprostatitisinmice AT fenglin obacunonealleviateschronicpelvicpainandproinflammatorydepolarizationofmacrophageinducedbyexperimentalautoimmuneprostatitisinmice AT chaozhaoliang obacunonealleviateschronicpelvicpainandproinflammatorydepolarizationofmacrophageinducedbyexperimentalautoimmuneprostatitisinmice |