A glimpse of the prokaryotic diversity of the Large Aral Sea reveals novel extremophilic bacterial and archaeal groups
Abstract During the last five decades, the Aral Sea has gradually changed from a saline water body to a hypersaline lake. Microbial community inhabiting the Aral Sea has been through a succession and continuous adaptation during the last 50 years of increasing salinization, but so far, the microbial...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2019-09-01
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| Series: | MicrobiologyOpen |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/mbo3.850 |
| _version_ | 1818010670630699008 |
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| author | Vyacheslav Shurigin Anna Hakobyan Hovik Panosyan Dilfuza Egamberdieva Kakhramon Davranov Nils‐Kåre Birkeland |
| author_facet | Vyacheslav Shurigin Anna Hakobyan Hovik Panosyan Dilfuza Egamberdieva Kakhramon Davranov Nils‐Kåre Birkeland |
| author_sort | Vyacheslav Shurigin |
| collection | DOAJ |
| description | Abstract During the last five decades, the Aral Sea has gradually changed from a saline water body to a hypersaline lake. Microbial community inhabiting the Aral Sea has been through a succession and continuous adaptation during the last 50 years of increasing salinization, but so far, the microbial diversity has not been explored. Prokaryotic diversity of the Large Aral Sea using cultivation‐independent methods based on determination of environmental 16S rRNA gene sequences revealed a microbial community related to typical marine or (hyper) saline‐adapted Bacteria and Archaea. The archaeal sequences were phylogenetically affiliated with the order Halobacteriales, with a large number of operational taxonomic units constituting a novel cluster in the Haloferacaceae family. Bacterial community analysis indicated a higher diversity with representatives belonging to Proteobacteria, Actinobacteria and Bacteroidetes. Many members of Alphaproteobacteria and Gammaproteobacteria were affiliated with genera like Roseovarius, Idiomarina and Spiribacter which have previously been found in marine or hypersaline waters. The majority of the phylotypes was most closely related to uncultivated organisms and shared less than 97% identity with their closest match in GenBank, indicating a unique community structure in the Large Aral Sea with mostly novel species or genera. |
| first_indexed | 2024-04-14T05:58:23Z |
| format | Article |
| id | doaj.art-dd46defcf32f4a3d937cce47939109a2 |
| institution | Directory Open Access Journal |
| issn | 2045-8827 |
| language | English |
| last_indexed | 2024-04-14T05:58:23Z |
| publishDate | 2019-09-01 |
| publisher | Wiley |
| record_format | Article |
| series | MicrobiologyOpen |
| spelling | doaj.art-dd46defcf32f4a3d937cce47939109a22022-12-22T02:08:52ZengWileyMicrobiologyOpen2045-88272019-09-0189n/an/a10.1002/mbo3.850A glimpse of the prokaryotic diversity of the Large Aral Sea reveals novel extremophilic bacterial and archaeal groupsVyacheslav Shurigin0Anna Hakobyan1Hovik Panosyan2Dilfuza Egamberdieva3Kakhramon Davranov4Nils‐Kåre Birkeland5Department of Biological Sciences University of Bergen Bergen NorwayDepartment of Biological Sciences University of Bergen Bergen NorwayDepartment of Biochemistry, Microbiology and Biotechnology Yerevan State University Yerevan ArmeniaDepartment of Microbiology and Biotechnology, Faculty of Biology National University of Uzbekistan Tashkent UzbekistanDepartment of Microbiology and Biotechnology, Faculty of Biology National University of Uzbekistan Tashkent UzbekistanDepartment of Biological Sciences University of Bergen Bergen NorwayAbstract During the last five decades, the Aral Sea has gradually changed from a saline water body to a hypersaline lake. Microbial community inhabiting the Aral Sea has been through a succession and continuous adaptation during the last 50 years of increasing salinization, but so far, the microbial diversity has not been explored. Prokaryotic diversity of the Large Aral Sea using cultivation‐independent methods based on determination of environmental 16S rRNA gene sequences revealed a microbial community related to typical marine or (hyper) saline‐adapted Bacteria and Archaea. The archaeal sequences were phylogenetically affiliated with the order Halobacteriales, with a large number of operational taxonomic units constituting a novel cluster in the Haloferacaceae family. Bacterial community analysis indicated a higher diversity with representatives belonging to Proteobacteria, Actinobacteria and Bacteroidetes. Many members of Alphaproteobacteria and Gammaproteobacteria were affiliated with genera like Roseovarius, Idiomarina and Spiribacter which have previously been found in marine or hypersaline waters. The majority of the phylotypes was most closely related to uncultivated organisms and shared less than 97% identity with their closest match in GenBank, indicating a unique community structure in the Large Aral Sea with mostly novel species or genera.https://doi.org/10.1002/mbo3.850archaeabacteriahalophilesphylogenyprokaryotic diversitysalt lake |
| spellingShingle | Vyacheslav Shurigin Anna Hakobyan Hovik Panosyan Dilfuza Egamberdieva Kakhramon Davranov Nils‐Kåre Birkeland A glimpse of the prokaryotic diversity of the Large Aral Sea reveals novel extremophilic bacterial and archaeal groups MicrobiologyOpen archaea bacteria halophiles phylogeny prokaryotic diversity salt lake |
| title | A glimpse of the prokaryotic diversity of the Large Aral Sea reveals novel extremophilic bacterial and archaeal groups |
| title_full | A glimpse of the prokaryotic diversity of the Large Aral Sea reveals novel extremophilic bacterial and archaeal groups |
| title_fullStr | A glimpse of the prokaryotic diversity of the Large Aral Sea reveals novel extremophilic bacterial and archaeal groups |
| title_full_unstemmed | A glimpse of the prokaryotic diversity of the Large Aral Sea reveals novel extremophilic bacterial and archaeal groups |
| title_short | A glimpse of the prokaryotic diversity of the Large Aral Sea reveals novel extremophilic bacterial and archaeal groups |
| title_sort | glimpse of the prokaryotic diversity of the large aral sea reveals novel extremophilic bacterial and archaeal groups |
| topic | archaea bacteria halophiles phylogeny prokaryotic diversity salt lake |
| url | https://doi.org/10.1002/mbo3.850 |
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