An Arabidopsis zinc finger protein increases abiotic stress tolerance by regulating sodium and potassium homeostasis, reactive oxygen species scavenging and osmotic potential
Plant zinc finger proteins (ZFPs) comprise a large protein family and they mainly involve in abiotic stress tolerance. Although Arabidopsis RING/FYVE/PHD ZFP (At5g62460; AtRZFP) is found to bind to zinc, whether it is involved in abiotic stress tolerance is unknown. In the present study, we characte...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2016-08-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fpls.2016.01272/full |
| _version_ | 1818840800673398784 |
|---|---|
| author | Dandan Zang Hongyan Li Hongyun Xu Wenhui Zhang Yiming Zhang Xinxin Shi Yucheng Wang Yucheng Wang |
| author_facet | Dandan Zang Hongyan Li Hongyun Xu Wenhui Zhang Yiming Zhang Xinxin Shi Yucheng Wang Yucheng Wang |
| author_sort | Dandan Zang |
| collection | DOAJ |
| description | Plant zinc finger proteins (ZFPs) comprise a large protein family and they mainly involve in abiotic stress tolerance. Although Arabidopsis RING/FYVE/PHD ZFP (At5g62460; AtRZFP) is found to bind to zinc, whether it is involved in abiotic stress tolerance is unknown. In the present study, we characterized the roles of AtRZFP in response to abiotic stresses. The expression of AtRZFP was induced significantly by salt and osmotic stress. AtRZFP positively mediates tolerance to salt and osmotic stress. Additionally, compared with wild-type Arabidopsis plants, plants overexpressing AtRZFP showed reduced reactive oxygen species accumulation, enhanced superoxide dismutase and peroxidase activity, increased soluble sugars and proline contents, reduced K+ loss, decreased Na+ accumulation, stomatal aperture and the water loss rate. Conversely, AtRZFP knockout plants displayed the opposite physiological changes when exposed to salt or osmotic stress conditions. These data suggested that AtRZFP enhances salt and osmotic tolerance through a series of physiological processes, including enhanced reactive oxygen species scavenging, maintaining Na+ and K+ homeostasis, controlling the stomatal aperture to reduce the water loss rate, and accumulating soluble sugars and proline to adjust the osmotic potential. |
| first_indexed | 2024-12-19T04:15:57Z |
| format | Article |
| id | doaj.art-dd48cfb07a004d84960f0d330b31d008 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-19T04:15:57Z |
| publishDate | 2016-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-dd48cfb07a004d84960f0d330b31d0082022-12-21T20:36:18ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2016-08-01710.3389/fpls.2016.01272214765An Arabidopsis zinc finger protein increases abiotic stress tolerance by regulating sodium and potassium homeostasis, reactive oxygen species scavenging and osmotic potentialDandan Zang0Hongyan Li1Hongyun Xu2Wenhui Zhang3Yiming Zhang4Xinxin Shi5Yucheng Wang6Yucheng Wang7State Key Laboratory of Forest Genetics and Tree Breeding (Northeast Forestry University)State Key Laboratory of Forest Genetics and Tree Breeding (Northeast Forestry University)State Key Laboratory of Forest Genetics and Tree Breeding (Northeast Forestry University)State Key Laboratory of Forest Genetics and Tree Breeding (Northeast Forestry University)State Key Laboratory of Forest Genetics and Tree Breeding (Northeast Forestry University)State Key Laboratory of Forest Genetics and Tree Breeding (Northeast Forestry University)Key Laboratory of Biogeography and Bioresource in Arid LandState Key Laboratory of Forest Genetics and Tree Breeding (Northeast Forestry University)Plant zinc finger proteins (ZFPs) comprise a large protein family and they mainly involve in abiotic stress tolerance. Although Arabidopsis RING/FYVE/PHD ZFP (At5g62460; AtRZFP) is found to bind to zinc, whether it is involved in abiotic stress tolerance is unknown. In the present study, we characterized the roles of AtRZFP in response to abiotic stresses. The expression of AtRZFP was induced significantly by salt and osmotic stress. AtRZFP positively mediates tolerance to salt and osmotic stress. Additionally, compared with wild-type Arabidopsis plants, plants overexpressing AtRZFP showed reduced reactive oxygen species accumulation, enhanced superoxide dismutase and peroxidase activity, increased soluble sugars and proline contents, reduced K+ loss, decreased Na+ accumulation, stomatal aperture and the water loss rate. Conversely, AtRZFP knockout plants displayed the opposite physiological changes when exposed to salt or osmotic stress conditions. These data suggested that AtRZFP enhances salt and osmotic tolerance through a series of physiological processes, including enhanced reactive oxygen species scavenging, maintaining Na+ and K+ homeostasis, controlling the stomatal aperture to reduce the water loss rate, and accumulating soluble sugars and proline to adjust the osmotic potential.http://journal.frontiersin.org/Journal/10.3389/fpls.2016.01272/fullArabidopsis thalianaabiotic stressZinc finger proteinsNa+ and K+ ContentAtRZFPROS scavenging analysis |
| spellingShingle | Dandan Zang Hongyan Li Hongyun Xu Wenhui Zhang Yiming Zhang Xinxin Shi Yucheng Wang Yucheng Wang An Arabidopsis zinc finger protein increases abiotic stress tolerance by regulating sodium and potassium homeostasis, reactive oxygen species scavenging and osmotic potential Frontiers in Plant Science Arabidopsis thaliana abiotic stress Zinc finger proteins Na+ and K+ Content AtRZFP ROS scavenging analysis |
| title | An Arabidopsis zinc finger protein increases abiotic stress tolerance by regulating sodium and potassium homeostasis, reactive oxygen species scavenging and osmotic potential |
| title_full | An Arabidopsis zinc finger protein increases abiotic stress tolerance by regulating sodium and potassium homeostasis, reactive oxygen species scavenging and osmotic potential |
| title_fullStr | An Arabidopsis zinc finger protein increases abiotic stress tolerance by regulating sodium and potassium homeostasis, reactive oxygen species scavenging and osmotic potential |
| title_full_unstemmed | An Arabidopsis zinc finger protein increases abiotic stress tolerance by regulating sodium and potassium homeostasis, reactive oxygen species scavenging and osmotic potential |
| title_short | An Arabidopsis zinc finger protein increases abiotic stress tolerance by regulating sodium and potassium homeostasis, reactive oxygen species scavenging and osmotic potential |
| title_sort | arabidopsis zinc finger protein increases abiotic stress tolerance by regulating sodium and potassium homeostasis reactive oxygen species scavenging and osmotic potential |
| topic | Arabidopsis thaliana abiotic stress Zinc finger proteins Na+ and K+ Content AtRZFP ROS scavenging analysis |
| url | http://journal.frontiersin.org/Journal/10.3389/fpls.2016.01272/full |
| work_keys_str_mv | AT dandanzang anarabidopsiszincfingerproteinincreasesabioticstresstolerancebyregulatingsodiumandpotassiumhomeostasisreactiveoxygenspeciesscavengingandosmoticpotential AT hongyanli anarabidopsiszincfingerproteinincreasesabioticstresstolerancebyregulatingsodiumandpotassiumhomeostasisreactiveoxygenspeciesscavengingandosmoticpotential AT hongyunxu anarabidopsiszincfingerproteinincreasesabioticstresstolerancebyregulatingsodiumandpotassiumhomeostasisreactiveoxygenspeciesscavengingandosmoticpotential AT wenhuizhang anarabidopsiszincfingerproteinincreasesabioticstresstolerancebyregulatingsodiumandpotassiumhomeostasisreactiveoxygenspeciesscavengingandosmoticpotential AT yimingzhang anarabidopsiszincfingerproteinincreasesabioticstresstolerancebyregulatingsodiumandpotassiumhomeostasisreactiveoxygenspeciesscavengingandosmoticpotential AT xinxinshi anarabidopsiszincfingerproteinincreasesabioticstresstolerancebyregulatingsodiumandpotassiumhomeostasisreactiveoxygenspeciesscavengingandosmoticpotential AT yuchengwang anarabidopsiszincfingerproteinincreasesabioticstresstolerancebyregulatingsodiumandpotassiumhomeostasisreactiveoxygenspeciesscavengingandosmoticpotential AT yuchengwang anarabidopsiszincfingerproteinincreasesabioticstresstolerancebyregulatingsodiumandpotassiumhomeostasisreactiveoxygenspeciesscavengingandosmoticpotential AT dandanzang arabidopsiszincfingerproteinincreasesabioticstresstolerancebyregulatingsodiumandpotassiumhomeostasisreactiveoxygenspeciesscavengingandosmoticpotential AT hongyanli arabidopsiszincfingerproteinincreasesabioticstresstolerancebyregulatingsodiumandpotassiumhomeostasisreactiveoxygenspeciesscavengingandosmoticpotential AT hongyunxu arabidopsiszincfingerproteinincreasesabioticstresstolerancebyregulatingsodiumandpotassiumhomeostasisreactiveoxygenspeciesscavengingandosmoticpotential AT wenhuizhang arabidopsiszincfingerproteinincreasesabioticstresstolerancebyregulatingsodiumandpotassiumhomeostasisreactiveoxygenspeciesscavengingandosmoticpotential AT yimingzhang arabidopsiszincfingerproteinincreasesabioticstresstolerancebyregulatingsodiumandpotassiumhomeostasisreactiveoxygenspeciesscavengingandosmoticpotential AT xinxinshi arabidopsiszincfingerproteinincreasesabioticstresstolerancebyregulatingsodiumandpotassiumhomeostasisreactiveoxygenspeciesscavengingandosmoticpotential AT yuchengwang arabidopsiszincfingerproteinincreasesabioticstresstolerancebyregulatingsodiumandpotassiumhomeostasisreactiveoxygenspeciesscavengingandosmoticpotential AT yuchengwang arabidopsiszincfingerproteinincreasesabioticstresstolerancebyregulatingsodiumandpotassiumhomeostasisreactiveoxygenspeciesscavengingandosmoticpotential |