Intra-Articular Route for the System of Molecules 14G1862 from <i>Centella asiatica</i>: Pain Relieving and Protective Effects in a Rat Model of Osteoarthritis
Current pharmacological therapies for the management of chronic articular diseases are far from being satisfactory, so new strategies need to be investigated. We tested the intra-articular pain relieving properties of a system of molecules from a characterized <i>Centella asiatica</i> ex...
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MDPI AG
2020-05-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/12/6/1618 |
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| author | Laura Micheli Lorenzo Di Cesare Mannelli Luisa Mattoli Sara Tamimi Enrico Flamini Stefano Garetto Jacopo Lucci Emiliano Giovagnoni Lorenzo Cinci Mario D’Ambrosio Cristina Luceri Carla Ghelardini |
| author_facet | Laura Micheli Lorenzo Di Cesare Mannelli Luisa Mattoli Sara Tamimi Enrico Flamini Stefano Garetto Jacopo Lucci Emiliano Giovagnoni Lorenzo Cinci Mario D’Ambrosio Cristina Luceri Carla Ghelardini |
| author_sort | Laura Micheli |
| collection | DOAJ |
| description | Current pharmacological therapies for the management of chronic articular diseases are far from being satisfactory, so new strategies need to be investigated. We tested the intra-articular pain relieving properties of a system of molecules from a characterized <i>Centella asiatica</i> extract (14G1862) in a rat model of osteoarthritis induced by monoiodoacetate (MIA). 14G1862 (0.2–2 mg mL<sup>−1</sup>) was intra-articularly (i.a.) injected 7 days after MIA, behavioural and histological evaluations were performed 14, 30 and 60 days after treatments. Moreover, the effect of 14G1862 on nitrate production and iNOS expression in RAW 264.7 macrophages stimulated with LPS was assessed. In vitro, 14G1862 treatment attenuated LPS-induced NO production and iNOS expression in a comparable manner to celecoxib. In vivo, 14G1862 significantly reduced mechanical allodynia and hyperalgesia, spontaneous pain and motor alterations starting on day 14 up to day 60. The efficacy was higher or comparable to that evoked by triamcinolone acetonide (100 μg i.a.) used as reference drug. Histological evaluation highlighted the improvement of several morphological parameters in MIA + 14G1862-treated animals with particularly benefic effects on joint space and fibrin deposition. In conclusion, i.a. treatment with <i>Centella asiatica</i> is a candidate to be a novel effective approach for osteoarthritis therapy. |
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| id | doaj.art-dd4c5c0ac4d7451ba8e96ca818391098 |
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| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T19:28:11Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-dd4c5c0ac4d7451ba8e96ca8183910982023-11-20T02:24:42ZengMDPI AGNutrients2072-66432020-05-01126161810.3390/nu12061618Intra-Articular Route for the System of Molecules 14G1862 from <i>Centella asiatica</i>: Pain Relieving and Protective Effects in a Rat Model of OsteoarthritisLaura Micheli0Lorenzo Di Cesare Mannelli1Luisa Mattoli2Sara Tamimi3Enrico Flamini4Stefano Garetto5Jacopo Lucci6Emiliano Giovagnoni7Lorenzo Cinci8Mario D’Ambrosio9Cristina Luceri10Carla Ghelardini11Department of Neuroscience, Psychology, Drug Research and Child Health-NEUROFARBA-Pharmacology and Toxicology Section, University of Florence, Viale Gaetano Pieraccini 6, 50139 Florence, ItalyDepartment of Neuroscience, Psychology, Drug Research and Child Health-NEUROFARBA-Pharmacology and Toxicology Section, University of Florence, Viale Gaetano Pieraccini 6, 50139 Florence, ItalyAboca SpA Società Agricola, Innovation & Medical Science Division, Loc. Aboca 20, 52037 Sansepolcro, ItalyAboca SpA Società Agricola, Innovation & Medical Science Division, Loc. Aboca 20, 52037 Sansepolcro, ItalyAboca SpA Società Agricola, Innovation & Medical Science Division, Loc. Aboca 20, 52037 Sansepolcro, ItalyAboca SpA Società Agricola, Innovation & Medical Science Division, Loc. Aboca 20, 52037 Sansepolcro, ItalyAboca SpA Società Agricola, Innovation & Medical Science Division, Loc. Aboca 20, 52037 Sansepolcro, ItalyAboca SpA Società Agricola, Innovation & Medical Science Division, Loc. Aboca 20, 52037 Sansepolcro, ItalyDepartment of Neuroscience, Psychology, Drug Research and Child Health-NEUROFARBA-Pharmacology and Toxicology Section, University of Florence, Viale Gaetano Pieraccini 6, 50139 Florence, ItalyDepartment of Neuroscience, Psychology, Drug Research and Child Health-NEUROFARBA-Pharmacology and Toxicology Section, University of Florence, Viale Gaetano Pieraccini 6, 50139 Florence, ItalyDepartment of Neuroscience, Psychology, Drug Research and Child Health-NEUROFARBA-Pharmacology and Toxicology Section, University of Florence, Viale Gaetano Pieraccini 6, 50139 Florence, ItalyDepartment of Neuroscience, Psychology, Drug Research and Child Health-NEUROFARBA-Pharmacology and Toxicology Section, University of Florence, Viale Gaetano Pieraccini 6, 50139 Florence, ItalyCurrent pharmacological therapies for the management of chronic articular diseases are far from being satisfactory, so new strategies need to be investigated. We tested the intra-articular pain relieving properties of a system of molecules from a characterized <i>Centella asiatica</i> extract (14G1862) in a rat model of osteoarthritis induced by monoiodoacetate (MIA). 14G1862 (0.2–2 mg mL<sup>−1</sup>) was intra-articularly (i.a.) injected 7 days after MIA, behavioural and histological evaluations were performed 14, 30 and 60 days after treatments. Moreover, the effect of 14G1862 on nitrate production and iNOS expression in RAW 264.7 macrophages stimulated with LPS was assessed. In vitro, 14G1862 treatment attenuated LPS-induced NO production and iNOS expression in a comparable manner to celecoxib. In vivo, 14G1862 significantly reduced mechanical allodynia and hyperalgesia, spontaneous pain and motor alterations starting on day 14 up to day 60. The efficacy was higher or comparable to that evoked by triamcinolone acetonide (100 μg i.a.) used as reference drug. Histological evaluation highlighted the improvement of several morphological parameters in MIA + 14G1862-treated animals with particularly benefic effects on joint space and fibrin deposition. In conclusion, i.a. treatment with <i>Centella asiatica</i> is a candidate to be a novel effective approach for osteoarthritis therapy.https://www.mdpi.com/2072-6643/12/6/1618<i>Centella asiatica</i>intra-articular treatmentosteoarthritisMIARAW 264.7 cell line14G1862 |
| spellingShingle | Laura Micheli Lorenzo Di Cesare Mannelli Luisa Mattoli Sara Tamimi Enrico Flamini Stefano Garetto Jacopo Lucci Emiliano Giovagnoni Lorenzo Cinci Mario D’Ambrosio Cristina Luceri Carla Ghelardini Intra-Articular Route for the System of Molecules 14G1862 from <i>Centella asiatica</i>: Pain Relieving and Protective Effects in a Rat Model of Osteoarthritis Nutrients <i>Centella asiatica</i> intra-articular treatment osteoarthritis MIA RAW 264.7 cell line 14G1862 |
| title | Intra-Articular Route for the System of Molecules 14G1862 from <i>Centella asiatica</i>: Pain Relieving and Protective Effects in a Rat Model of Osteoarthritis |
| title_full | Intra-Articular Route for the System of Molecules 14G1862 from <i>Centella asiatica</i>: Pain Relieving and Protective Effects in a Rat Model of Osteoarthritis |
| title_fullStr | Intra-Articular Route for the System of Molecules 14G1862 from <i>Centella asiatica</i>: Pain Relieving and Protective Effects in a Rat Model of Osteoarthritis |
| title_full_unstemmed | Intra-Articular Route for the System of Molecules 14G1862 from <i>Centella asiatica</i>: Pain Relieving and Protective Effects in a Rat Model of Osteoarthritis |
| title_short | Intra-Articular Route for the System of Molecules 14G1862 from <i>Centella asiatica</i>: Pain Relieving and Protective Effects in a Rat Model of Osteoarthritis |
| title_sort | intra articular route for the system of molecules 14g1862 from i centella asiatica i pain relieving and protective effects in a rat model of osteoarthritis |
| topic | <i>Centella asiatica</i> intra-articular treatment osteoarthritis MIA RAW 264.7 cell line 14G1862 |
| url | https://www.mdpi.com/2072-6643/12/6/1618 |
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