EXPRESSION OF TH17-LYMPHOCYTE ADAPTER – TRAF3IP2 IN THE SKIN OF PSORIASIS PATIENTS
Abstract. TRAF3IP2 gene plays an important role in the processes of inflammation and autoimmune control. TRAF3IP2 protein acts as a signal transducer from membrane receptors to the cell nucleus and is essential for the biological effects of Th17-lymphocytes. The goal of present study was to investig...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | Russian |
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St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists
2014-07-01
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| Series: | Медицинская иммунология |
| Subjects: | |
| Online Access: | https://www.mimmun.ru/mimmun/article/view/604 |
| _version_ | 1826534290108710912 |
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| author | V. R. Khairutdinov A. F. Mikhailichenko A. A. Piskunova N. V. Bychkova A. V. Samtsov E. N. Imyanitov |
| author_facet | V. R. Khairutdinov A. F. Mikhailichenko A. A. Piskunova N. V. Bychkova A. V. Samtsov E. N. Imyanitov |
| author_sort | V. R. Khairutdinov |
| collection | DOAJ |
| description | Abstract. TRAF3IP2 gene plays an important role in the processes of inflammation and autoimmune control. TRAF3IP2 protein acts as a signal transducer from membrane receptors to the cell nucleus and is essential for the biological effects of Th17-lymphocytes. The goal of present study was to investigate distribution of allelic variants of TRAF3IP2 Arg74Trp polymorphism as a risk factor in psoriasis development, and to evaluate TRAF3IP2 mRNA expression levels in psoriasis patients and healthy donors. Peripheral blood leukocytes were used as a source of DNA. Gene testing was performed for 261 patients and 482 healthy blood donors by real-time allele-specific PCR. TRAF3IP2 mRNA expression was measured in the skin biopsy samples from 36 patients with psoriasis and 14 healthy donors by means of quantitative reverse transcriptase PCR, using a paired-sample approach with SDHA reference gene. We have observed a significantly increased occurrence of rare Trp allele, with 10.5% among psoriasis patients (55/522), as compared with 7.0% (67/964) for healthy persons (OR = 1.58, 95% CI: 1.09 – 2.28, р = 0.02). A trend of higher Trp allele occurrence was revealed in patients with severe psoriasis – 21/154 (13.6%) when compared to the patients with milder course of the disease – 34/368 (9.2%) (р = 0.14). No differences were found for TRAF3IP2 mRNA expression in skin samples from psoriasis patients versus healthy persons. The association between TRAF3IP2 Trp allele and increased susceptibility to psoriasis suggests that Trp variant of this protein may display a higher enzymatic activity. Induction of inflammatory process in carriers of Trp allele would be more intensive, thus being a predisposing factor in psoriasis development. (Med. Immunol., 2011, vol. 13, N 6, pp 597-602) |
| first_indexed | 2024-03-08T05:51:08Z |
| format | Article |
| id | doaj.art-dd4d014f451743a1b787323635b6582c |
| institution | Directory Open Access Journal |
| issn | 1563-0625 2313-741X |
| language | Russian |
| last_indexed | 2025-03-14T02:20:40Z |
| publishDate | 2014-07-01 |
| publisher | St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists |
| record_format | Article |
| series | Медицинская иммунология |
| spelling | doaj.art-dd4d014f451743a1b787323635b6582c2025-03-11T17:59:04ZrusSt. Petersburg branch of the Russian Association of Allergologists and Clinical ImmunologistsМедицинская иммунология1563-06252313-741X2014-07-0113659760210.15789/1563-0625-2011-6-597-602601EXPRESSION OF TH17-LYMPHOCYTE ADAPTER – TRAF3IP2 IN THE SKIN OF PSORIASIS PATIENTSV. R. Khairutdinov0A. F. Mikhailichenko1A. A. Piskunova2N. V. Bychkova3A. V. Samtsov4E. N. Imyanitov5Военно-медицинская академия им. С.М. Кирова, Санкт-ПетербургВоенно-медицинская академия им. С.М. Кирова, Санкт-ПетербургСанкт-Петербургская Медицинская академия последипломного образования, Санкт-ПетербургВЦЭРМ им. А.М. Никифорова МЧС РФ, Санкт-ПетербургВоенно-медицинская академия им. С.М. Кирова, Санкт-ПетербургНаучно-исследовательский институт онкологии им. Н.Н. Петрова, Санкт-ПетербургAbstract. TRAF3IP2 gene plays an important role in the processes of inflammation and autoimmune control. TRAF3IP2 protein acts as a signal transducer from membrane receptors to the cell nucleus and is essential for the biological effects of Th17-lymphocytes. The goal of present study was to investigate distribution of allelic variants of TRAF3IP2 Arg74Trp polymorphism as a risk factor in psoriasis development, and to evaluate TRAF3IP2 mRNA expression levels in psoriasis patients and healthy donors. Peripheral blood leukocytes were used as a source of DNA. Gene testing was performed for 261 patients and 482 healthy blood donors by real-time allele-specific PCR. TRAF3IP2 mRNA expression was measured in the skin biopsy samples from 36 patients with psoriasis and 14 healthy donors by means of quantitative reverse transcriptase PCR, using a paired-sample approach with SDHA reference gene. We have observed a significantly increased occurrence of rare Trp allele, with 10.5% among psoriasis patients (55/522), as compared with 7.0% (67/964) for healthy persons (OR = 1.58, 95% CI: 1.09 – 2.28, р = 0.02). A trend of higher Trp allele occurrence was revealed in patients with severe psoriasis – 21/154 (13.6%) when compared to the patients with milder course of the disease – 34/368 (9.2%) (р = 0.14). No differences were found for TRAF3IP2 mRNA expression in skin samples from psoriasis patients versus healthy persons. The association between TRAF3IP2 Trp allele and increased susceptibility to psoriasis suggests that Trp variant of this protein may display a higher enzymatic activity. Induction of inflammatory process in carriers of Trp allele would be more intensive, thus being a predisposing factor in psoriasis development. (Med. Immunol., 2011, vol. 13, N 6, pp 597-602)https://www.mimmun.ru/mimmun/article/view/604psoriasistraf3ip2 genepredisposalgene expressiongene polymorphism |
| spellingShingle | V. R. Khairutdinov A. F. Mikhailichenko A. A. Piskunova N. V. Bychkova A. V. Samtsov E. N. Imyanitov EXPRESSION OF TH17-LYMPHOCYTE ADAPTER – TRAF3IP2 IN THE SKIN OF PSORIASIS PATIENTS Медицинская иммунология psoriasis traf3ip2 gene predisposal gene expression gene polymorphism |
| title | EXPRESSION OF TH17-LYMPHOCYTE ADAPTER – TRAF3IP2 IN THE SKIN OF PSORIASIS PATIENTS |
| title_full | EXPRESSION OF TH17-LYMPHOCYTE ADAPTER – TRAF3IP2 IN THE SKIN OF PSORIASIS PATIENTS |
| title_fullStr | EXPRESSION OF TH17-LYMPHOCYTE ADAPTER – TRAF3IP2 IN THE SKIN OF PSORIASIS PATIENTS |
| title_full_unstemmed | EXPRESSION OF TH17-LYMPHOCYTE ADAPTER – TRAF3IP2 IN THE SKIN OF PSORIASIS PATIENTS |
| title_short | EXPRESSION OF TH17-LYMPHOCYTE ADAPTER – TRAF3IP2 IN THE SKIN OF PSORIASIS PATIENTS |
| title_sort | expression of th17 lymphocyte adapter traf3ip2 in the skin of psoriasis patients |
| topic | psoriasis traf3ip2 gene predisposal gene expression gene polymorphism |
| url | https://www.mimmun.ru/mimmun/article/view/604 |
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