Application of Magnetically Assisted Reactors for Modulation of Growth and Pyocyanin Production by Pseudomonas aeruginosa
Pseudomonas aeruginosa is a producer of desired secondary metabolites, including pyocyanin. Potential uses of this pigment urge a search for improved production methods. Recent trends in bioprocessing show the potential of the use of electromagnetic fields (EMFs) to influence the growth of microorga...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-03-01
|
| Series: | Frontiers in Bioengineering and Biotechnology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2022.795871/full |
| _version_ | 1819349487582183424 |
|---|---|
| author | Joanna Jabłońska Kamila Dubrowska Adrian Augustyniak Adrian Augustyniak Marian Kordas Rafał Rakoczy |
| author_facet | Joanna Jabłońska Kamila Dubrowska Adrian Augustyniak Adrian Augustyniak Marian Kordas Rafał Rakoczy |
| author_sort | Joanna Jabłońska |
| collection | DOAJ |
| description | Pseudomonas aeruginosa is a producer of desired secondary metabolites, including pyocyanin. Potential uses of this pigment urge a search for improved production methods. Recent trends in bioprocessing show the potential of the use of electromagnetic fields (EMFs) to influence the growth of microorganisms and even modulate the concentration of bioproducts. Here, we aimed at assessing the influence of rotating magnetic field (RMF) and static magnetic field (SMF) on pyocyanin production, growth rate, and respiration of P. aeruginosa. Moreover, exposure time to EMFs (2, 6, and 12 h) and culture volume (10 and 50 ml) were initially assessed. P. aeruginosa was cultivated in magnetically assisted reactors with 5 and 50 Hz RMF (magnetic induction of 24.32 and 42.64 mT, respectively) and SMF (−17.37 mT). Growth kinetics was assessed with Gompertz equation. The viability was tested using resazurin assay, whereas pyocyanin production by chloroform-HCl methodology. The growth of P. aeruginosa was slightly stimulated by exposure to a RMF with 50 Hz (108% related to the control) and significantly by SMF (132% related to the control), while RMF 5 Hz exposure prolonged the time of inflection (in comparison to RMF 50 Hz and SMF). The 6-h exposure to EMFs resulted in the highest pyocyanin production in comparison to the control, indicating a relationship between exposure time and product concentration. Moreover, cultures led in smaller volumes produced more pyocyanin. Our findings show that the use of different EMF types, frequency, and exposition time and volume could be used interchangeably to obtain different bioprocess aims. |
| first_indexed | 2024-12-24T19:01:18Z |
| format | Article |
| id | doaj.art-dd51668658f44f9a8e741476e6b73bb2 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-24T19:01:18Z |
| publishDate | 2022-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-dd51668658f44f9a8e741476e6b73bb22022-12-21T16:43:13ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852022-03-011010.3389/fbioe.2022.795871795871Application of Magnetically Assisted Reactors for Modulation of Growth and Pyocyanin Production by Pseudomonas aeruginosaJoanna Jabłońska0Kamila Dubrowska1Adrian Augustyniak2Adrian Augustyniak3Marian Kordas4Rafał Rakoczy5Department of Chemical and Process Engineering, Faculty of Chemical Technology and Engineering, West Pomeranian University of Technology in Szczecin, Szczecin, PolandDepartment of Chemical and Process Engineering, Faculty of Chemical Technology and Engineering, West Pomeranian University of Technology in Szczecin, Szczecin, PolandDepartment of Chemical and Process Engineering, Faculty of Chemical Technology and Engineering, West Pomeranian University of Technology in Szczecin, Szczecin, PolandChair of Building Materials and Construction Chemistry, Technische Universität Berlin, Berlin, GermanyDepartment of Chemical and Process Engineering, Faculty of Chemical Technology and Engineering, West Pomeranian University of Technology in Szczecin, Szczecin, PolandDepartment of Chemical and Process Engineering, Faculty of Chemical Technology and Engineering, West Pomeranian University of Technology in Szczecin, Szczecin, PolandPseudomonas aeruginosa is a producer of desired secondary metabolites, including pyocyanin. Potential uses of this pigment urge a search for improved production methods. Recent trends in bioprocessing show the potential of the use of electromagnetic fields (EMFs) to influence the growth of microorganisms and even modulate the concentration of bioproducts. Here, we aimed at assessing the influence of rotating magnetic field (RMF) and static magnetic field (SMF) on pyocyanin production, growth rate, and respiration of P. aeruginosa. Moreover, exposure time to EMFs (2, 6, and 12 h) and culture volume (10 and 50 ml) were initially assessed. P. aeruginosa was cultivated in magnetically assisted reactors with 5 and 50 Hz RMF (magnetic induction of 24.32 and 42.64 mT, respectively) and SMF (−17.37 mT). Growth kinetics was assessed with Gompertz equation. The viability was tested using resazurin assay, whereas pyocyanin production by chloroform-HCl methodology. The growth of P. aeruginosa was slightly stimulated by exposure to a RMF with 50 Hz (108% related to the control) and significantly by SMF (132% related to the control), while RMF 5 Hz exposure prolonged the time of inflection (in comparison to RMF 50 Hz and SMF). The 6-h exposure to EMFs resulted in the highest pyocyanin production in comparison to the control, indicating a relationship between exposure time and product concentration. Moreover, cultures led in smaller volumes produced more pyocyanin. Our findings show that the use of different EMF types, frequency, and exposition time and volume could be used interchangeably to obtain different bioprocess aims.https://www.frontiersin.org/articles/10.3389/fbioe.2022.795871/fullphenazinesbioprocessingrotating electromagnetic fieldstatic electromagnetic fieldbacterial growth |
| spellingShingle | Joanna Jabłońska Kamila Dubrowska Adrian Augustyniak Adrian Augustyniak Marian Kordas Rafał Rakoczy Application of Magnetically Assisted Reactors for Modulation of Growth and Pyocyanin Production by Pseudomonas aeruginosa Frontiers in Bioengineering and Biotechnology phenazines bioprocessing rotating electromagnetic field static electromagnetic field bacterial growth |
| title | Application of Magnetically Assisted Reactors for Modulation of Growth and Pyocyanin Production by Pseudomonas aeruginosa |
| title_full | Application of Magnetically Assisted Reactors for Modulation of Growth and Pyocyanin Production by Pseudomonas aeruginosa |
| title_fullStr | Application of Magnetically Assisted Reactors for Modulation of Growth and Pyocyanin Production by Pseudomonas aeruginosa |
| title_full_unstemmed | Application of Magnetically Assisted Reactors for Modulation of Growth and Pyocyanin Production by Pseudomonas aeruginosa |
| title_short | Application of Magnetically Assisted Reactors for Modulation of Growth and Pyocyanin Production by Pseudomonas aeruginosa |
| title_sort | application of magnetically assisted reactors for modulation of growth and pyocyanin production by pseudomonas aeruginosa |
| topic | phenazines bioprocessing rotating electromagnetic field static electromagnetic field bacterial growth |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2022.795871/full |
| work_keys_str_mv | AT joannajabłonska applicationofmagneticallyassistedreactorsformodulationofgrowthandpyocyaninproductionbypseudomonasaeruginosa AT kamiladubrowska applicationofmagneticallyassistedreactorsformodulationofgrowthandpyocyaninproductionbypseudomonasaeruginosa AT adrianaugustyniak applicationofmagneticallyassistedreactorsformodulationofgrowthandpyocyaninproductionbypseudomonasaeruginosa AT adrianaugustyniak applicationofmagneticallyassistedreactorsformodulationofgrowthandpyocyaninproductionbypseudomonasaeruginosa AT mariankordas applicationofmagneticallyassistedreactorsformodulationofgrowthandpyocyaninproductionbypseudomonasaeruginosa AT rafałrakoczy applicationofmagneticallyassistedreactorsformodulationofgrowthandpyocyaninproductionbypseudomonasaeruginosa |