Corneal proteome and differentially expressed corneal proteins in highly myopic chicks using a label-free SWATH-MS quantification approach
Abstract Myopia, or short-sightedness, is a highly prevalent refractive disorder in which the eye’s focal length is too short for its axial dimension in its relaxed state. High myopia is associated with increased risks of blinding ocular complications and abnormal eye shape. In addition to consisten...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-03-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-84904-4 |
| _version_ | 1818435819159945216 |
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| author | Byung Soo Kang Thomas Chuen Lam Jimmy Ka-wai Cheung King Kit Li Chea-su Kee |
| author_facet | Byung Soo Kang Thomas Chuen Lam Jimmy Ka-wai Cheung King Kit Li Chea-su Kee |
| author_sort | Byung Soo Kang |
| collection | DOAJ |
| description | Abstract Myopia, or short-sightedness, is a highly prevalent refractive disorder in which the eye’s focal length is too short for its axial dimension in its relaxed state. High myopia is associated with increased risks of blinding ocular complications and abnormal eye shape. In addition to consistent findings on posterior segment anomalies in high myopia (e.g., scleral remodeling), more recent biometric and biomechanical data in myopic humans and animal models also indicate anterior segment anomalies (e.g., corneal biomechanical properties). Because the cornea is the anterior-most ocular tissue, providing essential refractive power and physiological stability, it is important to understand the biochemical signaling pathway during myopia development. This study first aimed to establish the entire chicken corneal proteome. Then, using the classical form deprivation paradigm to induce high myopia in chicks, state-of-the-art bioinformatics technologies were applied to identify eight differentially expressed proteins in the highly myopic cornea. These results provide strong foundation for future corneal research, especially those using chicken as an animal model for myopia development. |
| first_indexed | 2024-12-14T16:58:56Z |
| format | Article |
| id | doaj.art-dd546f60ec30486cbb72a1b692078316 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-14T16:58:56Z |
| publishDate | 2021-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-dd546f60ec30486cbb72a1b6920783162022-12-21T22:53:53ZengNature PortfolioScientific Reports2045-23222021-03-0111111210.1038/s41598-021-84904-4Corneal proteome and differentially expressed corneal proteins in highly myopic chicks using a label-free SWATH-MS quantification approachByung Soo Kang0Thomas Chuen Lam1Jimmy Ka-wai Cheung2King Kit Li3Chea-su Kee4School of Optometry, The Hong Kong Polytechnic UniversitySchool of Optometry, The Hong Kong Polytechnic UniversitySchool of Optometry, The Hong Kong Polytechnic UniversitySchool of Optometry, The Hong Kong Polytechnic UniversitySchool of Optometry, The Hong Kong Polytechnic UniversityAbstract Myopia, or short-sightedness, is a highly prevalent refractive disorder in which the eye’s focal length is too short for its axial dimension in its relaxed state. High myopia is associated with increased risks of blinding ocular complications and abnormal eye shape. In addition to consistent findings on posterior segment anomalies in high myopia (e.g., scleral remodeling), more recent biometric and biomechanical data in myopic humans and animal models also indicate anterior segment anomalies (e.g., corneal biomechanical properties). Because the cornea is the anterior-most ocular tissue, providing essential refractive power and physiological stability, it is important to understand the biochemical signaling pathway during myopia development. This study first aimed to establish the entire chicken corneal proteome. Then, using the classical form deprivation paradigm to induce high myopia in chicks, state-of-the-art bioinformatics technologies were applied to identify eight differentially expressed proteins in the highly myopic cornea. These results provide strong foundation for future corneal research, especially those using chicken as an animal model for myopia development.https://doi.org/10.1038/s41598-021-84904-4 |
| spellingShingle | Byung Soo Kang Thomas Chuen Lam Jimmy Ka-wai Cheung King Kit Li Chea-su Kee Corneal proteome and differentially expressed corneal proteins in highly myopic chicks using a label-free SWATH-MS quantification approach Scientific Reports |
| title | Corneal proteome and differentially expressed corneal proteins in highly myopic chicks using a label-free SWATH-MS quantification approach |
| title_full | Corneal proteome and differentially expressed corneal proteins in highly myopic chicks using a label-free SWATH-MS quantification approach |
| title_fullStr | Corneal proteome and differentially expressed corneal proteins in highly myopic chicks using a label-free SWATH-MS quantification approach |
| title_full_unstemmed | Corneal proteome and differentially expressed corneal proteins in highly myopic chicks using a label-free SWATH-MS quantification approach |
| title_short | Corneal proteome and differentially expressed corneal proteins in highly myopic chicks using a label-free SWATH-MS quantification approach |
| title_sort | corneal proteome and differentially expressed corneal proteins in highly myopic chicks using a label free swath ms quantification approach |
| url | https://doi.org/10.1038/s41598-021-84904-4 |
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