Assessing Salinity Tolerance in Rice Mutants by Phenotypic Evaluation Alongside Simple Sequence Repeat Analysis
Salinity stress is one of the most severe constraints limiting rice production worldwide. Thus, the development of salt-tolerant rice promises to deal with increasing food demand due to climate change effects. This study investigated the salinity tolerance of mutant rice by evaluating phenotype and...
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MDPI AG
2020-05-01
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| Series: | Agriculture |
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| Online Access: | https://www.mdpi.com/2077-0472/10/6/191 |
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| author | Can Thu Huong Truong Thi Tu Anh Hoang-Dung Tran Vu Xuan Duong Nguyen Thanh Trung Tran Dang Khanh Tran Dang Xuan |
| author_facet | Can Thu Huong Truong Thi Tu Anh Hoang-Dung Tran Vu Xuan Duong Nguyen Thanh Trung Tran Dang Khanh Tran Dang Xuan |
| author_sort | Can Thu Huong |
| collection | DOAJ |
| description | Salinity stress is one of the most severe constraints limiting rice production worldwide. Thus, the development of salt-tolerant rice promises to deal with increasing food demand due to climate change effects. This study investigated the salinity tolerance of mutant rice by evaluating phenotype and genotype, using forty-two simple sequence repeat (SSR) markers linked to the salinity tolerance <i>Saltol</i> quantitative trait locus (QTL) in ten cultivars and mutant lines. Results of phenotypic screening showed that the mutant line SKLo/BC15TB and cultivar BC15TB performed salt tolerance, while the mutant line Bao Thai/DT 84 and cultivar DT84DB were sensitive to salt stress. The markers RM 493, RM 562, RM 10748, RM 518, RM 237, and RM 20224 were the most polymorphic in salinity tolerance. Among them, RM 237, RM 10748, and RM 224 showed the highest polymorphism information (PIC = 0.58). This study reveals that the three markers are profitable for classification of salinity tolerance in both cultivar and mutant rice. The mutant line SKLo/BC15TB and cultivar BC15TB were found to be promising candidates for diversity analysis of salt-tolerant rice. Findings of this study are useful for developing new salinity-tolerant rice cultivars towards climate change. |
| first_indexed | 2024-03-10T19:34:09Z |
| format | Article |
| id | doaj.art-dd59dab4856149219022772a258a0c2e |
| institution | Directory Open Access Journal |
| issn | 2077-0472 |
| language | English |
| last_indexed | 2024-03-10T19:34:09Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agriculture |
| spelling | doaj.art-dd59dab4856149219022772a258a0c2e2023-11-20T01:53:20ZengMDPI AGAgriculture2077-04722020-05-0110619110.3390/agriculture10060191Assessing Salinity Tolerance in Rice Mutants by Phenotypic Evaluation Alongside Simple Sequence Repeat AnalysisCan Thu Huong0Truong Thi Tu Anh1Hoang-Dung Tran2Vu Xuan Duong3Nguyen Thanh Trung4Tran Dang Khanh5Tran Dang Xuan6Transdisciplinary Science and Engineering Program (Development Science Field), Graduate School for Advanced Science and Engineering, Development Science Field, Hiroshima University, 1-5-1 Kagamiyama, Higashi-Hiroshima City, Hiroshima 739-8529, JapanDivision of Biotechnology, Institute of Agricultural Sciences for Southern Vietnam, 121 Nguyen Binh Khiem Street, District 1, Ho Chi Minh City 71058, VietnamFaculty of Biotechnology, Nguyen Tat Thanh University, 298A-300A Nguyen Tat Thanh Street, Ward 13, District 4, Ho Chi Minh City 72820, VietnamInstitute of Applied Research and Development, Hung Vuong University, Viet Tri City 291470, VietnamFaculty of Pharmacy, Institute of Research and Development, Duy Tan University, Da Nang 550000, VietnamDepartment of Genetic Engineering, Agricultural Genetics Institute, Pham Van Dong Street, Hanoi 122000, VietnamTransdisciplinary Science and Engineering Program (Development Science Field), Graduate School for Advanced Science and Engineering, Development Science Field, Hiroshima University, 1-5-1 Kagamiyama, Higashi-Hiroshima City, Hiroshima 739-8529, JapanSalinity stress is one of the most severe constraints limiting rice production worldwide. Thus, the development of salt-tolerant rice promises to deal with increasing food demand due to climate change effects. This study investigated the salinity tolerance of mutant rice by evaluating phenotype and genotype, using forty-two simple sequence repeat (SSR) markers linked to the salinity tolerance <i>Saltol</i> quantitative trait locus (QTL) in ten cultivars and mutant lines. Results of phenotypic screening showed that the mutant line SKLo/BC15TB and cultivar BC15TB performed salt tolerance, while the mutant line Bao Thai/DT 84 and cultivar DT84DB were sensitive to salt stress. The markers RM 493, RM 562, RM 10748, RM 518, RM 237, and RM 20224 were the most polymorphic in salinity tolerance. Among them, RM 237, RM 10748, and RM 224 showed the highest polymorphism information (PIC = 0.58). This study reveals that the three markers are profitable for classification of salinity tolerance in both cultivar and mutant rice. The mutant line SKLo/BC15TB and cultivar BC15TB were found to be promising candidates for diversity analysis of salt-tolerant rice. Findings of this study are useful for developing new salinity-tolerant rice cultivars towards climate change.https://www.mdpi.com/2077-0472/10/6/191salinity stressmutant ricemorphologicalSSR markersgenotypephenotype |
| spellingShingle | Can Thu Huong Truong Thi Tu Anh Hoang-Dung Tran Vu Xuan Duong Nguyen Thanh Trung Tran Dang Khanh Tran Dang Xuan Assessing Salinity Tolerance in Rice Mutants by Phenotypic Evaluation Alongside Simple Sequence Repeat Analysis Agriculture salinity stress mutant rice morphological SSR markers genotype phenotype |
| title | Assessing Salinity Tolerance in Rice Mutants by Phenotypic Evaluation Alongside Simple Sequence Repeat Analysis |
| title_full | Assessing Salinity Tolerance in Rice Mutants by Phenotypic Evaluation Alongside Simple Sequence Repeat Analysis |
| title_fullStr | Assessing Salinity Tolerance in Rice Mutants by Phenotypic Evaluation Alongside Simple Sequence Repeat Analysis |
| title_full_unstemmed | Assessing Salinity Tolerance in Rice Mutants by Phenotypic Evaluation Alongside Simple Sequence Repeat Analysis |
| title_short | Assessing Salinity Tolerance in Rice Mutants by Phenotypic Evaluation Alongside Simple Sequence Repeat Analysis |
| title_sort | assessing salinity tolerance in rice mutants by phenotypic evaluation alongside simple sequence repeat analysis |
| topic | salinity stress mutant rice morphological SSR markers genotype phenotype |
| url | https://www.mdpi.com/2077-0472/10/6/191 |
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