A comparison of cotton and flocked swabs for vaginal self-sample collection
Manuela Viviano,1 Alexia Willame,1 Marie Cohen,1 Anne-Caroline Benski,1 Rosa Catarino,1 Christine Wuillemin,1 Phuong Lien Tran,1 Patrick Petignat,1 Pierre Vassilakos2 1Division of Gynecology, Geneva University Hospitals, Geneva, Switzerland; 2Geneva Foundation for Medical Education and Research, Ge...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Dove Medical Press
2018-05-01
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| Series: | International Journal of Women's Health |
| Subjects: | |
| Online Access: | https://www.dovepress.com/a-comparison-of-cotton-and-flocked-swabs-for-vaginal-self-sample-colle-peer-reviewed-article-IJWH |
| _version_ | 1818318752539738112 |
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| author | Viviano M Willame A Cohen M Benski AC Catarino R Wuillemin C Tran PL Petignat P Vassilakos P |
| author_facet | Viviano M Willame A Cohen M Benski AC Catarino R Wuillemin C Tran PL Petignat P Vassilakos P |
| author_sort | Viviano M |
| collection | DOAJ |
| description | Manuela Viviano,1 Alexia Willame,1 Marie Cohen,1 Anne-Caroline Benski,1 Rosa Catarino,1 Christine Wuillemin,1 Phuong Lien Tran,1 Patrick Petignat,1 Pierre Vassilakos2 1Division of Gynecology, Geneva University Hospitals, Geneva, Switzerland; 2Geneva Foundation for Medical Education and Research, Geneva, Switzerland Objective: Vaginal self-sampling for human papillomavirus (HPV) testing has recently been proposed to optimize cervical cancer screening coverage. The objective of this study was to compare the performance of self-taken samples using flocked and cotton swabs for HPV detection and cellular retrieval.Methods: We recruited women aged 21–65 years, referred to colposcopy at the Division of Gynecology of the Geneva University Hospitals between May and September 2016. Each participant collected 2 vaginal samples: 1 with a cotton swab and 1 with a flocked swab. A 1:1 randomization determined the order in which the 2 samples were taken. The swabs were introduced into a 20 mL PreservCyt® vial. Real-time polymerase chain reaction analysis using the Anyplex™ II HPV HR assay, cytofluorometric analysis and cytological cell counting were performed on each sample.Results: A total of 119 participants were recruited in the study. Their mean ± standard deviation age was 35.1±8.9 years. The HPV prevalence was 29.7% and 38.1% according to the cotton and flocked swab, respectively (p=0.006). The mean number of cells collected per milliliter according to cytofluorometry was 96,726.6 with the cotton swab and 425,544.3 with the flocked swab (p<0.001). The mean number of cells detected at cytological cell count was 13,130.42 using the cotton swab and 17,503.6 using the flocked swab (p<0.001). Conclusion: The flocked swab achieved a greater cellular retrieval and showed an improved performance in HPV detection. Further studies are needed to assess the usability and cost-effectiveness of the 2 self-sampling devices. Keywords: cervical neoplasia, cervical cytology, human papillomavirus, screening, flocked swab, HPV testing |
| first_indexed | 2024-12-13T09:58:13Z |
| format | Article |
| id | doaj.art-dd5a8a5f916e43c9ba136b705c2776b8 |
| institution | Directory Open Access Journal |
| issn | 1179-1411 |
| language | English |
| last_indexed | 2024-12-13T09:58:13Z |
| publishDate | 2018-05-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of Women's Health |
| spelling | doaj.art-dd5a8a5f916e43c9ba136b705c2776b82022-12-21T23:51:44ZengDove Medical PressInternational Journal of Women's Health1179-14112018-05-01Volume 1022923638303A comparison of cotton and flocked swabs for vaginal self-sample collectionViviano MWillame ACohen MBenski ACCatarino RWuillemin CTran PLPetignat PVassilakos PManuela Viviano,1 Alexia Willame,1 Marie Cohen,1 Anne-Caroline Benski,1 Rosa Catarino,1 Christine Wuillemin,1 Phuong Lien Tran,1 Patrick Petignat,1 Pierre Vassilakos2 1Division of Gynecology, Geneva University Hospitals, Geneva, Switzerland; 2Geneva Foundation for Medical Education and Research, Geneva, Switzerland Objective: Vaginal self-sampling for human papillomavirus (HPV) testing has recently been proposed to optimize cervical cancer screening coverage. The objective of this study was to compare the performance of self-taken samples using flocked and cotton swabs for HPV detection and cellular retrieval.Methods: We recruited women aged 21–65 years, referred to colposcopy at the Division of Gynecology of the Geneva University Hospitals between May and September 2016. Each participant collected 2 vaginal samples: 1 with a cotton swab and 1 with a flocked swab. A 1:1 randomization determined the order in which the 2 samples were taken. The swabs were introduced into a 20 mL PreservCyt® vial. Real-time polymerase chain reaction analysis using the Anyplex™ II HPV HR assay, cytofluorometric analysis and cytological cell counting were performed on each sample.Results: A total of 119 participants were recruited in the study. Their mean ± standard deviation age was 35.1±8.9 years. The HPV prevalence was 29.7% and 38.1% according to the cotton and flocked swab, respectively (p=0.006). The mean number of cells collected per milliliter according to cytofluorometry was 96,726.6 with the cotton swab and 425,544.3 with the flocked swab (p<0.001). The mean number of cells detected at cytological cell count was 13,130.42 using the cotton swab and 17,503.6 using the flocked swab (p<0.001). Conclusion: The flocked swab achieved a greater cellular retrieval and showed an improved performance in HPV detection. Further studies are needed to assess the usability and cost-effectiveness of the 2 self-sampling devices. Keywords: cervical neoplasia, cervical cytology, human papillomavirus, screening, flocked swab, HPV testinghttps://www.dovepress.com/a-comparison-of-cotton-and-flocked-swabs-for-vaginal-self-sample-colle-peer-reviewed-article-IJWHcervical neoplasiacervical cytologyhuman papillomavirusscreening |
| spellingShingle | Viviano M Willame A Cohen M Benski AC Catarino R Wuillemin C Tran PL Petignat P Vassilakos P A comparison of cotton and flocked swabs for vaginal self-sample collection International Journal of Women's Health cervical neoplasia cervical cytology human papillomavirus screening |
| title | A comparison of cotton and flocked swabs for vaginal self-sample collection |
| title_full | A comparison of cotton and flocked swabs for vaginal self-sample collection |
| title_fullStr | A comparison of cotton and flocked swabs for vaginal self-sample collection |
| title_full_unstemmed | A comparison of cotton and flocked swabs for vaginal self-sample collection |
| title_short | A comparison of cotton and flocked swabs for vaginal self-sample collection |
| title_sort | comparison of cotton and flocked swabs for vaginal self sample collection |
| topic | cervical neoplasia cervical cytology human papillomavirus screening |
| url | https://www.dovepress.com/a-comparison-of-cotton-and-flocked-swabs-for-vaginal-self-sample-colle-peer-reviewed-article-IJWH |
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