Structural and Predicted Functional Diversities of Bacterial Microbiome in Response to Sewage Sludge Amendment in Coastal Mudflat Soil
The study investigated the influence of sewage sludge application at rates of 0 (CK), 30 (ST), 75 (MT), and 150 (HT) t ha<sup>−1</sup> to mudflats on bacterial community diversity and predicted functions using amplicon-based sequencing. Soils under sewage sludge treatments, especially th...
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MDPI AG
2021-12-01
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| Online Access: | https://www.mdpi.com/2079-7737/10/12/1302 |
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| author | Yunlong Li Yimin Wang Chao Shen Lu Xu Siqiang Yi Yilin Zhao Wengang Zuo Chuanhui Gu Yuhua Shan Yanchao Bai |
| author_facet | Yunlong Li Yimin Wang Chao Shen Lu Xu Siqiang Yi Yilin Zhao Wengang Zuo Chuanhui Gu Yuhua Shan Yanchao Bai |
| author_sort | Yunlong Li |
| collection | DOAJ |
| description | The study investigated the influence of sewage sludge application at rates of 0 (CK), 30 (ST), 75 (MT), and 150 (HT) t ha<sup>−1</sup> to mudflats on bacterial community diversity and predicted functions using amplicon-based sequencing. Soils under sewage sludge treatments, especially the HT treatment, exhibited lower pH, salinity and higher nutrient contents (C, N, and P). Moreover, restructured bacterial communities with significantly higher diversities and distinct core and unique microbiomes were observed in all sewage sludge-amended soils as compared to the control. Specifically, core bacterial families, such as <i>Hyphomicrobiaceae</i>, <i>Cytophagaceae</i>, <i>Pirellulaceae Microbacteriaceae</i>, and <i>Phyllobacteriaceae</i>, were significantly enriched in sewage sludge-amended soils. In addition, sewage sludge amendment significantly improved predicted functional diversities of core microbiomes, with significantly higher accumulative relative abundances of functions related to carbon and nitrogen cycling processes compared to the unamended treatment. Correlation analyses showed that modified soil physicochemical properties were conducive for the improvement of diversities of bacterial communities and predicted functionalities. These outcomes demonstrated that sewage sludge amendment not only alleviated saline–sodic and nutrient deficiency conditions, but also restructured bacterial communities with higher diversities and versatile functions, which may be particularly important for the fertility formation and development of mudflat soils. |
| first_indexed | 2024-03-10T04:35:10Z |
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| id | doaj.art-dd5b954bb0fc4959a39156b46760cedc |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-10T04:35:10Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-dd5b954bb0fc4959a39156b46760cedc2023-11-23T03:53:59ZengMDPI AGBiology2079-77372021-12-011012130210.3390/biology10121302Structural and Predicted Functional Diversities of Bacterial Microbiome in Response to Sewage Sludge Amendment in Coastal Mudflat SoilYunlong Li0Yimin Wang1Chao Shen2Lu Xu3Siqiang Yi4Yilin Zhao5Wengang Zuo6Chuanhui Gu7Yuhua Shan8Yanchao Bai9College of Environmental Science and Engineering, Yangzhou University, Yangzhou 225127, ChinaCollege of Environmental Science and Engineering, Yangzhou University, Yangzhou 225127, ChinaCollege of Environmental Science and Engineering, Yangzhou University, Yangzhou 225127, ChinaCollege of Environmental Science and Engineering, Yangzhou University, Yangzhou 225127, ChinaCollege of Environmental Science and Engineering, Yangzhou University, Yangzhou 225127, ChinaCollege of Environmental Science and Engineering, Yangzhou University, Yangzhou 225127, ChinaCollege of Environmental Science and Engineering, Yangzhou University, Yangzhou 225127, ChinaEnvironmental Research Center, Duke Kunshan University, Kunshan 215316, ChinaCollege of Environmental Science and Engineering, Yangzhou University, Yangzhou 225127, ChinaCollege of Environmental Science and Engineering, Yangzhou University, Yangzhou 225127, ChinaThe study investigated the influence of sewage sludge application at rates of 0 (CK), 30 (ST), 75 (MT), and 150 (HT) t ha<sup>−1</sup> to mudflats on bacterial community diversity and predicted functions using amplicon-based sequencing. Soils under sewage sludge treatments, especially the HT treatment, exhibited lower pH, salinity and higher nutrient contents (C, N, and P). Moreover, restructured bacterial communities with significantly higher diversities and distinct core and unique microbiomes were observed in all sewage sludge-amended soils as compared to the control. Specifically, core bacterial families, such as <i>Hyphomicrobiaceae</i>, <i>Cytophagaceae</i>, <i>Pirellulaceae Microbacteriaceae</i>, and <i>Phyllobacteriaceae</i>, were significantly enriched in sewage sludge-amended soils. In addition, sewage sludge amendment significantly improved predicted functional diversities of core microbiomes, with significantly higher accumulative relative abundances of functions related to carbon and nitrogen cycling processes compared to the unamended treatment. Correlation analyses showed that modified soil physicochemical properties were conducive for the improvement of diversities of bacterial communities and predicted functionalities. These outcomes demonstrated that sewage sludge amendment not only alleviated saline–sodic and nutrient deficiency conditions, but also restructured bacterial communities with higher diversities and versatile functions, which may be particularly important for the fertility formation and development of mudflat soils.https://www.mdpi.com/2079-7737/10/12/1302coastal mudflatsaline–sodic soilsewage sludgemicrobial diversitybacterial communitycore and unique microbiomes |
| spellingShingle | Yunlong Li Yimin Wang Chao Shen Lu Xu Siqiang Yi Yilin Zhao Wengang Zuo Chuanhui Gu Yuhua Shan Yanchao Bai Structural and Predicted Functional Diversities of Bacterial Microbiome in Response to Sewage Sludge Amendment in Coastal Mudflat Soil Biology coastal mudflat saline–sodic soil sewage sludge microbial diversity bacterial community core and unique microbiomes |
| title | Structural and Predicted Functional Diversities of Bacterial Microbiome in Response to Sewage Sludge Amendment in Coastal Mudflat Soil |
| title_full | Structural and Predicted Functional Diversities of Bacterial Microbiome in Response to Sewage Sludge Amendment in Coastal Mudflat Soil |
| title_fullStr | Structural and Predicted Functional Diversities of Bacterial Microbiome in Response to Sewage Sludge Amendment in Coastal Mudflat Soil |
| title_full_unstemmed | Structural and Predicted Functional Diversities of Bacterial Microbiome in Response to Sewage Sludge Amendment in Coastal Mudflat Soil |
| title_short | Structural and Predicted Functional Diversities of Bacterial Microbiome in Response to Sewage Sludge Amendment in Coastal Mudflat Soil |
| title_sort | structural and predicted functional diversities of bacterial microbiome in response to sewage sludge amendment in coastal mudflat soil |
| topic | coastal mudflat saline–sodic soil sewage sludge microbial diversity bacterial community core and unique microbiomes |
| url | https://www.mdpi.com/2079-7737/10/12/1302 |
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