Achromobacter sp. Strain BUKˍBCHˍTQ1: A Potential Paraquat-Degrading Bacterium Isolated from Pesticide Contaminated Agricultural Soil
Paraquat (1, 1′-dimethyl-4, 4′-bipyridinium dichloride) is one of the most frequently used herbicide in agriculture. It is a cationic non-systematic, non-selective contact compound that instantaneously interferes with the photosynthetic processes of plants. It has an immediate effect, once the compo...
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| Format: | Article |
| Language: | English |
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Penteract Technology
2023-11-01
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| Series: | Malaysian Journal of Science and Advanced Technology |
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| Online Access: | http://www.mjsat.com.my/index.php/mjsat/article/view/205 |
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| author | Aisha Aliyu Tukur Jahun Bashir Muhammad Muhammad Yahuza Gimba Abdulrahman Abdulhamid Arabo Aisha Muhammad Ado Dayyabu Shehu |
| author_facet | Aisha Aliyu Tukur Jahun Bashir Muhammad Muhammad Yahuza Gimba Abdulrahman Abdulhamid Arabo Aisha Muhammad Ado Dayyabu Shehu |
| author_sort | Aisha Aliyu Tukur |
| collection | DOAJ |
| description | Paraquat (1, 1′-dimethyl-4, 4′-bipyridinium dichloride) is one of the most frequently used herbicide in agriculture. It is a cationic non-systematic, non-selective contact compound that instantaneously interferes with the photosynthetic processes of plants. It has an immediate effect, once the compound comes into contact with the plants’ leaves, where the reaction occurs. However, the contamination of paraquat residue in soil can harm soil microbes, flora and fauna, farmer health and also soil ecology, which affects the soil fertility. The objective of this study was to isolate and characterize bacteria with the ability to break down and utilize paraquat as the primary carbon source. The isolation process involved the enrichment of mineral salt media (MSM) using serial dilution. The isolated bacterium underwent morphological, biochemical, and molecular identification following characterization. Results showed the isolate was identified as Achromobacter sp. with the accession number OQ372943 based on partial 16S rRNA gene sequence and phylogenetic analysis. The growth and degradation of paraquat by this isolate were optimum at a pH of 6.5, 276 mgL-1 of the substrate (paraquat), temperature of 35 °C, 200 µL of biomass size, and 48 h of incubation. The degradation efficiency of the isolate after 120 hours of incubation under optimal conditions was 91.01%. Hence, these results demonstrated a very high efficiency of paraquat degradation. Consequently, this isolate holds significant promise for paraquat degradation and could serve as a viable contender for remediating paraquat-contaminated environments.
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| first_indexed | 2024-03-09T14:47:50Z |
| format | Article |
| id | doaj.art-dd5d52d6946d42c3b2c4c90c0606cded |
| institution | Directory Open Access Journal |
| issn | 2785-8901 |
| language | English |
| last_indexed | 2024-03-09T14:47:50Z |
| publishDate | 2023-11-01 |
| publisher | Penteract Technology |
| record_format | Article |
| series | Malaysian Journal of Science and Advanced Technology |
| spelling | doaj.art-dd5d52d6946d42c3b2c4c90c0606cded2023-11-26T21:10:55ZengPenteract TechnologyMalaysian Journal of Science and Advanced Technology2785-89012023-11-013410.56532/mjsat.v3i4.205Achromobacter sp. Strain BUKˍBCHˍTQ1: A Potential Paraquat-Degrading Bacterium Isolated from Pesticide Contaminated Agricultural SoilAisha Aliyu Tukur0Jahun Bashir Muhammad 1Muhammad Yahuza Gimba2Abdulrahman Abdulhamid Arabo3Aisha Muhammad Ado4Dayyabu Shehu5Department of Biochemistry, Faculty of Basic Medical Sciences, Bayero University, Kano, Nigeria.Department of Biology, Federal College of Education, Technical, Bichi, Kano, NigeriaDepartment of Biochemistry, Faculty of Basic Medical Sciences, Bayero University, Kano, Nigeria.Department of Science Laboratory Technology, Adamawa State Polytechnic, Yola, NigeriaDepartment of Biochemistry, Faculty of Basic Medical Sciences, Bayero University, Kano, Nigeria.Department of Biochemistry, Faculty of Basic Medical Sciences, Bayero University, Kano, Nigeria.Paraquat (1, 1′-dimethyl-4, 4′-bipyridinium dichloride) is one of the most frequently used herbicide in agriculture. It is a cationic non-systematic, non-selective contact compound that instantaneously interferes with the photosynthetic processes of plants. It has an immediate effect, once the compound comes into contact with the plants’ leaves, where the reaction occurs. However, the contamination of paraquat residue in soil can harm soil microbes, flora and fauna, farmer health and also soil ecology, which affects the soil fertility. The objective of this study was to isolate and characterize bacteria with the ability to break down and utilize paraquat as the primary carbon source. The isolation process involved the enrichment of mineral salt media (MSM) using serial dilution. The isolated bacterium underwent morphological, biochemical, and molecular identification following characterization. Results showed the isolate was identified as Achromobacter sp. with the accession number OQ372943 based on partial 16S rRNA gene sequence and phylogenetic analysis. The growth and degradation of paraquat by this isolate were optimum at a pH of 6.5, 276 mgL-1 of the substrate (paraquat), temperature of 35 °C, 200 µL of biomass size, and 48 h of incubation. The degradation efficiency of the isolate after 120 hours of incubation under optimal conditions was 91.01%. Hence, these results demonstrated a very high efficiency of paraquat degradation. Consequently, this isolate holds significant promise for paraquat degradation and could serve as a viable contender for remediating paraquat-contaminated environments. http://www.mjsat.com.my/index.php/mjsat/article/view/205Keyword Agricultural SoilKeyword BioremediationKeyword ParaquatKeyword Achromobacter sp. |
| spellingShingle | Aisha Aliyu Tukur Jahun Bashir Muhammad Muhammad Yahuza Gimba Abdulrahman Abdulhamid Arabo Aisha Muhammad Ado Dayyabu Shehu Achromobacter sp. Strain BUKˍBCHˍTQ1: A Potential Paraquat-Degrading Bacterium Isolated from Pesticide Contaminated Agricultural Soil Malaysian Journal of Science and Advanced Technology Keyword Agricultural Soil Keyword Bioremediation Keyword Paraquat Keyword Achromobacter sp. |
| title | Achromobacter sp. Strain BUKˍBCHˍTQ1: A Potential Paraquat-Degrading Bacterium Isolated from Pesticide Contaminated Agricultural Soil |
| title_full | Achromobacter sp. Strain BUKˍBCHˍTQ1: A Potential Paraquat-Degrading Bacterium Isolated from Pesticide Contaminated Agricultural Soil |
| title_fullStr | Achromobacter sp. Strain BUKˍBCHˍTQ1: A Potential Paraquat-Degrading Bacterium Isolated from Pesticide Contaminated Agricultural Soil |
| title_full_unstemmed | Achromobacter sp. Strain BUKˍBCHˍTQ1: A Potential Paraquat-Degrading Bacterium Isolated from Pesticide Contaminated Agricultural Soil |
| title_short | Achromobacter sp. Strain BUKˍBCHˍTQ1: A Potential Paraquat-Degrading Bacterium Isolated from Pesticide Contaminated Agricultural Soil |
| title_sort | achromobacter sp strain bukˍbchˍtq1 a potential paraquat degrading bacterium isolated from pesticide contaminated agricultural soil |
| topic | Keyword Agricultural Soil Keyword Bioremediation Keyword Paraquat Keyword Achromobacter sp. |
| url | http://www.mjsat.com.my/index.php/mjsat/article/view/205 |
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