Preliminary Study of In Vitro Three-Dimensional Skin Model Using an Ovine Collagen Type I Sponge Seeded with Co-Culture Skin Cells: Submerged versus Air-Liquid Interface Conditions
Three-dimensional (3D) in vitro skin models have been widely used for cosmeceutical and pharmaceutical applications aiming to reduce animal use in experiment. This study investigate capability of ovine tendon collagen type I (OTC-I) sponge suitable platform for a 3D in vitro skin model using co-cult...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-11-01
|
| Series: | Polymers |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4360/12/12/2784 |
| _version_ | 1797546876639117312 |
|---|---|
| author | Mh Busra Fauzi Zahra Rashidbenam Aminuddin Bin Saim Ruszymah Binti Hj Idrus |
| author_facet | Mh Busra Fauzi Zahra Rashidbenam Aminuddin Bin Saim Ruszymah Binti Hj Idrus |
| author_sort | Mh Busra Fauzi |
| collection | DOAJ |
| description | Three-dimensional (3D) in vitro skin models have been widely used for cosmeceutical and pharmaceutical applications aiming to reduce animal use in experiment. This study investigate capability of ovine tendon collagen type I (OTC-I) sponge suitable platform for a 3D in vitro skin model using co-cultured skin cells (CC) containing human epidermal keratinocytes (HEK) and human dermal fibroblasts (HDF) under submerged (SM) and air-liquid interface (ALI) conditions. Briefly, the extracted OTC-I was freeze-dried and crosslinked with genipin (OTC-I_GNP) and carbodiimide (OTC-I_EDC). The gross appearance, physico-chemical characteristics, biocompatibility and growth profile of seeded skin cells were assessed. The light brown and white appearance for the OTC-I_GNP scaffold and other groups were observed, respectively. The OTC-I_GNP scaffold demonstrated the highest swelling ratio (~1885%) and water uptake (94.96 ± 0.14%). The Fourier transformation infrared demonstrated amide A, B and I, II and III which represent collagen type I. The microstructure of all fabricated sponges presented a similar surface roughness with the presence of visible collagen fibers and a heterogenous porous structure. The OTC-I_EDC scaffold was more toxic and showed the lowest cell attachment and proliferation as compared to other groups. The micrographic evaluation revealed that CC potentially formed the epidermal- and dermal-like layers in both SM and ALI that prominently observed with OTC-I_GNP compared to others. In conclusion, these results suggest that OTC_GNP could be used as a 3D in vitro skin model under ALI microenvironment. |
| first_indexed | 2024-03-10T14:35:25Z |
| format | Article |
| id | doaj.art-dd5eaeee1bcb463aa91da3546b694622 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-10T14:35:25Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-dd5eaeee1bcb463aa91da3546b6946222023-11-20T22:14:04ZengMDPI AGPolymers2073-43602020-11-011212278410.3390/polym12122784Preliminary Study of In Vitro Three-Dimensional Skin Model Using an Ovine Collagen Type I Sponge Seeded with Co-Culture Skin Cells: Submerged versus Air-Liquid Interface ConditionsMh Busra Fauzi0Zahra Rashidbenam1Aminuddin Bin Saim2Ruszymah Binti Hj Idrus3Centre for Tissue Engineering and Regenerative Medicine, Faculty of Medicine, UKM Medical Centre, Jalan Yaacob Latiff, Bandar Tun Razak, Kuala Lumpur 56000, MalaysiaCentre for Tissue Engineering and Regenerative Medicine, Faculty of Medicine, UKM Medical Centre, Jalan Yaacob Latiff, Bandar Tun Razak, Kuala Lumpur 56000, MalaysiaEar, Nose & Throat Consultant Clinic, Ampang Puteri Specialist Hospital, Taman Dato Ahmad Razali, Selangor 68000, MalaysiaCentre for Tissue Engineering and Regenerative Medicine, Faculty of Medicine, UKM Medical Centre, Jalan Yaacob Latiff, Bandar Tun Razak, Kuala Lumpur 56000, MalaysiaThree-dimensional (3D) in vitro skin models have been widely used for cosmeceutical and pharmaceutical applications aiming to reduce animal use in experiment. This study investigate capability of ovine tendon collagen type I (OTC-I) sponge suitable platform for a 3D in vitro skin model using co-cultured skin cells (CC) containing human epidermal keratinocytes (HEK) and human dermal fibroblasts (HDF) under submerged (SM) and air-liquid interface (ALI) conditions. Briefly, the extracted OTC-I was freeze-dried and crosslinked with genipin (OTC-I_GNP) and carbodiimide (OTC-I_EDC). The gross appearance, physico-chemical characteristics, biocompatibility and growth profile of seeded skin cells were assessed. The light brown and white appearance for the OTC-I_GNP scaffold and other groups were observed, respectively. The OTC-I_GNP scaffold demonstrated the highest swelling ratio (~1885%) and water uptake (94.96 ± 0.14%). The Fourier transformation infrared demonstrated amide A, B and I, II and III which represent collagen type I. The microstructure of all fabricated sponges presented a similar surface roughness with the presence of visible collagen fibers and a heterogenous porous structure. The OTC-I_EDC scaffold was more toxic and showed the lowest cell attachment and proliferation as compared to other groups. The micrographic evaluation revealed that CC potentially formed the epidermal- and dermal-like layers in both SM and ALI that prominently observed with OTC-I_GNP compared to others. In conclusion, these results suggest that OTC_GNP could be used as a 3D in vitro skin model under ALI microenvironment.https://www.mdpi.com/2073-4360/12/12/2784ovine collagencollagen type Igenipincarbodiimide3D skin modelair-liquid interface |
| spellingShingle | Mh Busra Fauzi Zahra Rashidbenam Aminuddin Bin Saim Ruszymah Binti Hj Idrus Preliminary Study of In Vitro Three-Dimensional Skin Model Using an Ovine Collagen Type I Sponge Seeded with Co-Culture Skin Cells: Submerged versus Air-Liquid Interface Conditions Polymers ovine collagen collagen type I genipin carbodiimide 3D skin model air-liquid interface |
| title | Preliminary Study of In Vitro Three-Dimensional Skin Model Using an Ovine Collagen Type I Sponge Seeded with Co-Culture Skin Cells: Submerged versus Air-Liquid Interface Conditions |
| title_full | Preliminary Study of In Vitro Three-Dimensional Skin Model Using an Ovine Collagen Type I Sponge Seeded with Co-Culture Skin Cells: Submerged versus Air-Liquid Interface Conditions |
| title_fullStr | Preliminary Study of In Vitro Three-Dimensional Skin Model Using an Ovine Collagen Type I Sponge Seeded with Co-Culture Skin Cells: Submerged versus Air-Liquid Interface Conditions |
| title_full_unstemmed | Preliminary Study of In Vitro Three-Dimensional Skin Model Using an Ovine Collagen Type I Sponge Seeded with Co-Culture Skin Cells: Submerged versus Air-Liquid Interface Conditions |
| title_short | Preliminary Study of In Vitro Three-Dimensional Skin Model Using an Ovine Collagen Type I Sponge Seeded with Co-Culture Skin Cells: Submerged versus Air-Liquid Interface Conditions |
| title_sort | preliminary study of in vitro three dimensional skin model using an ovine collagen type i sponge seeded with co culture skin cells submerged versus air liquid interface conditions |
| topic | ovine collagen collagen type I genipin carbodiimide 3D skin model air-liquid interface |
| url | https://www.mdpi.com/2073-4360/12/12/2784 |
| work_keys_str_mv | AT mhbusrafauzi preliminarystudyofinvitrothreedimensionalskinmodelusinganovinecollagentypeispongeseededwithcocultureskincellssubmergedversusairliquidinterfaceconditions AT zahrarashidbenam preliminarystudyofinvitrothreedimensionalskinmodelusinganovinecollagentypeispongeseededwithcocultureskincellssubmergedversusairliquidinterfaceconditions AT aminuddinbinsaim preliminarystudyofinvitrothreedimensionalskinmodelusinganovinecollagentypeispongeseededwithcocultureskincellssubmergedversusairliquidinterfaceconditions AT ruszymahbintihjidrus preliminarystudyofinvitrothreedimensionalskinmodelusinganovinecollagentypeispongeseededwithcocultureskincellssubmergedversusairliquidinterfaceconditions |