Interleukin 10 promoter haplotype is associated with alcoholic liver cirrhosis in Taiwanese patients
Alcoholic liver cirrhosis is a severe form of alcohol-related liver damage. More than 95% of heavy drinkers develop a fatty liver, but only 35% of them develop cirrhosis. We postulate that genetic factors may play a role in this difference. Genetic polymorphisms of the cytokine genes may influence K...
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Wiley
2014-06-01
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| Series: | Kaohsiung Journal of Medical Sciences |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1607551X14000692 |
| _version_ | 1811330489118621696 |
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| author | An-Ming Yang Li-Li Wen Chang-Shyue Yang Sun-Chong Wang Chien-Sheng Chen Ming-Jong Bair |
| author_facet | An-Ming Yang Li-Li Wen Chang-Shyue Yang Sun-Chong Wang Chien-Sheng Chen Ming-Jong Bair |
| author_sort | An-Ming Yang |
| collection | DOAJ |
| description | Alcoholic liver cirrhosis is a severe form of alcohol-related liver damage. More than 95% of heavy drinkers develop a fatty liver, but only 35% of them develop cirrhosis. We postulate that genetic factors may play a role in this difference. Genetic polymorphisms of the cytokine genes may influence Kupffer cells cytokine genes expression. In this study, we evaluated the promoter polymorphisms of interleukin (IL) 1β, IL 6, IL 10, and tumor necrosis factor alpha (TNFα) and aimed to clarify the association between the polymorphisms and the disease. Forty alcoholic patients with liver cirrhosis and 64 healthy volunteers were included in our investigation. Genotyping on IL 1β –511 T>C, IL 6 –572 G>C, IL 10 –819 C>T, IL 10 –1082 G>A, and TNFα –308 G>A was done. Another 36 patients with recurrent alcoholic pancreatitis were included as an additional control group. Genotyping on IL 10 –819 C>T and IL 10 –1082 G>A was done. The polymorphisms on IL 1 and IL 6 showed no significant association. The p value for TNFα –308 G>A was 0.028 in comparison with healthy volunteers. Although the p value was less than 0.05, it did not reach significance after Bonferroni correction. The p values for IL 10 –819 C>T and IL 10 –1082 G>A were respectively 0.031 and 0.026 in healthy volunteers and 0.028 and 0.023 in the alcoholic pancreatitis group. The results also did not reach significance after Bonferroni correction. Among the participants with the GCC haplotype, healthy volunteers had p = 0.027 (p < 0.05) and an odds ratio (OR) of 0.124 [confidence interval (95%) CI, 0.015–0.997], whereas the alcoholic pancreatitis group had p = 0.023 (p < 0.05) and an OR of 0.106 (95% CI, 0.012–0.912). The odds ratio of people having one ATA haplotype was 6.233 (95% CI, 0.739–52.547) in healthy volunteers and 6.588 (95% CI, 0.727–59.679) in the alcoholic pancreatitis group; the corresponding rate was 10.521 (95% CI, 1.252–88.440) and 12.833 (95% CI 1.408–117.008) for people with two ATA haplotypes. The p values in these groups were 0.031 (p < 0.05) and 0.028 (p < 0.05), respectively. The presence of a GCC haplotype could have protective effect against alcoholic liver disease, whereas the presence of an ATA haplotype could predispose carriers to the disease. The IL 10 promoter haplotype is associated with alcoholic liver cirrhosis in Taiwanese patients. |
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| format | Article |
| id | doaj.art-dd5ff69b77bb4f9f898bdd48abd2935d |
| institution | Directory Open Access Journal |
| issn | 1607-551X |
| language | English |
| last_indexed | 2024-04-13T16:03:54Z |
| publishDate | 2014-06-01 |
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| spelling | doaj.art-dd5ff69b77bb4f9f898bdd48abd2935d2022-12-22T02:40:28ZengWileyKaohsiung Journal of Medical Sciences1607-551X2014-06-0130629129810.1016/j.kjms.2014.02.016Interleukin 10 promoter haplotype is associated with alcoholic liver cirrhosis in Taiwanese patientsAn-Ming Yang0Li-Li Wen1Chang-Shyue Yang2Sun-Chong Wang3Chien-Sheng Chen4Ming-Jong Bair5Department of Internal Medicine, En Chu Kong Hospital, New Taipei City, TaiwanDepartment of Laboratory Medicine, En Chu Kong Hospital, New Taipei City, TaiwanDepartment of Internal Medicine, En Chu Kong Hospital, New Taipei City, TaiwanInstitute of Systems Biology and Bioinformatics, National Central University, Jhongli City, TaiwanInstitute of Systems Biology and Bioinformatics, National Central University, Jhongli City, TaiwanDepartment of Internal Medicine, Mackay Memorial Hospital, Taitung Branch, Taitung, TaiwanAlcoholic liver cirrhosis is a severe form of alcohol-related liver damage. More than 95% of heavy drinkers develop a fatty liver, but only 35% of them develop cirrhosis. We postulate that genetic factors may play a role in this difference. Genetic polymorphisms of the cytokine genes may influence Kupffer cells cytokine genes expression. In this study, we evaluated the promoter polymorphisms of interleukin (IL) 1β, IL 6, IL 10, and tumor necrosis factor alpha (TNFα) and aimed to clarify the association between the polymorphisms and the disease. Forty alcoholic patients with liver cirrhosis and 64 healthy volunteers were included in our investigation. Genotyping on IL 1β –511 T>C, IL 6 –572 G>C, IL 10 –819 C>T, IL 10 –1082 G>A, and TNFα –308 G>A was done. Another 36 patients with recurrent alcoholic pancreatitis were included as an additional control group. Genotyping on IL 10 –819 C>T and IL 10 –1082 G>A was done. The polymorphisms on IL 1 and IL 6 showed no significant association. The p value for TNFα –308 G>A was 0.028 in comparison with healthy volunteers. Although the p value was less than 0.05, it did not reach significance after Bonferroni correction. The p values for IL 10 –819 C>T and IL 10 –1082 G>A were respectively 0.031 and 0.026 in healthy volunteers and 0.028 and 0.023 in the alcoholic pancreatitis group. The results also did not reach significance after Bonferroni correction. Among the participants with the GCC haplotype, healthy volunteers had p = 0.027 (p < 0.05) and an odds ratio (OR) of 0.124 [confidence interval (95%) CI, 0.015–0.997], whereas the alcoholic pancreatitis group had p = 0.023 (p < 0.05) and an OR of 0.106 (95% CI, 0.012–0.912). The odds ratio of people having one ATA haplotype was 6.233 (95% CI, 0.739–52.547) in healthy volunteers and 6.588 (95% CI, 0.727–59.679) in the alcoholic pancreatitis group; the corresponding rate was 10.521 (95% CI, 1.252–88.440) and 12.833 (95% CI 1.408–117.008) for people with two ATA haplotypes. The p values in these groups were 0.031 (p < 0.05) and 0.028 (p < 0.05), respectively. The presence of a GCC haplotype could have protective effect against alcoholic liver disease, whereas the presence of an ATA haplotype could predispose carriers to the disease. The IL 10 promoter haplotype is associated with alcoholic liver cirrhosis in Taiwanese patients.http://www.sciencedirect.com/science/article/pii/S1607551X14000692Alcoholic liver cirrhosisIL 10 haplotypeIL 10 promoter polymorphismTNFα promoter polymorphism |
| spellingShingle | An-Ming Yang Li-Li Wen Chang-Shyue Yang Sun-Chong Wang Chien-Sheng Chen Ming-Jong Bair Interleukin 10 promoter haplotype is associated with alcoholic liver cirrhosis in Taiwanese patients Kaohsiung Journal of Medical Sciences Alcoholic liver cirrhosis IL 10 haplotype IL 10 promoter polymorphism TNFα promoter polymorphism |
| title | Interleukin 10 promoter haplotype is associated with alcoholic liver cirrhosis in Taiwanese patients |
| title_full | Interleukin 10 promoter haplotype is associated with alcoholic liver cirrhosis in Taiwanese patients |
| title_fullStr | Interleukin 10 promoter haplotype is associated with alcoholic liver cirrhosis in Taiwanese patients |
| title_full_unstemmed | Interleukin 10 promoter haplotype is associated with alcoholic liver cirrhosis in Taiwanese patients |
| title_short | Interleukin 10 promoter haplotype is associated with alcoholic liver cirrhosis in Taiwanese patients |
| title_sort | interleukin 10 promoter haplotype is associated with alcoholic liver cirrhosis in taiwanese patients |
| topic | Alcoholic liver cirrhosis IL 10 haplotype IL 10 promoter polymorphism TNFα promoter polymorphism |
| url | http://www.sciencedirect.com/science/article/pii/S1607551X14000692 |
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