Let-7 microRNAs Are Possibly Associated with Perineural Invasion in Colorectal Cancer by Targeting IGF Axis
Increased insulin-like growth factor (IGF) axis activity is associated with the development and progression of different types of malignancies, including colorectal cancer (CRC). MicroRNAs (miRNAs) belonging to the let-7 family have been reported to target genes involved in this axis and are known a...
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MDPI AG
2022-10-01
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| Online Access: | https://www.mdpi.com/2075-1729/12/10/1638 |
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| author | Andrei Marian Niculae Maria Dobre Vlad Herlea Teodora Ecaterina Manuc Bogdan Trandafir Elena Milanesi Mihail Eugen Hinescu |
| author_facet | Andrei Marian Niculae Maria Dobre Vlad Herlea Teodora Ecaterina Manuc Bogdan Trandafir Elena Milanesi Mihail Eugen Hinescu |
| author_sort | Andrei Marian Niculae |
| collection | DOAJ |
| description | Increased insulin-like growth factor (IGF) axis activity is associated with the development and progression of different types of malignancies, including colorectal cancer (CRC). MicroRNAs (miRNAs) belonging to the let-7 family have been reported to target genes involved in this axis and are known as tumor suppressors. In this study, in silico bioinformatic analysis was performed to assess miRNA–mRNA interactions between eight miRNAs belonging to the let-7 family and genes involved in the IGF signaling pathway, coding for receptors and substrates. miRNAs’ expression analysis revealed that hsa-let-7a-5p, hsa-let-7b-5p, hsa-let-7c-5p, hsa-let- 97 7d-5p, hsa-let-7e-5p, hsa-let-7f-5p, and hsa-let-7g-5p were significantly down-regulated in 25 CRC tumoral tissues (T) compared to the corresponding adjacent peritumoral tissues (PT). Moreover, our results showed an upregulation of miR-let-7e-5p in CRC tissues with mutations in KRAS codon 12 or 13, and, for the first time, found a specific dysregulation of let-7a-5p, let-7b-5p, let-7c-5p, let-7d-5p, and let-7i-5p in CRC with perineural invasion. Our results sustain the relationship between the IGF axis, let-7 miRNAs, and CRC and suggest an association between the expression of these miRNAs and perineural invasion. |
| first_indexed | 2024-03-09T19:56:16Z |
| format | Article |
| id | doaj.art-dd60a71afb0647f9bcd1e1b220fab38c |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-09T19:56:16Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-dd60a71afb0647f9bcd1e1b220fab38c2023-11-24T00:57:42ZengMDPI AGLife2075-17292022-10-011210163810.3390/life12101638Let-7 microRNAs Are Possibly Associated with Perineural Invasion in Colorectal Cancer by Targeting IGF AxisAndrei Marian Niculae0Maria Dobre1Vlad Herlea2Teodora Ecaterina Manuc3Bogdan Trandafir4Elena Milanesi5Mihail Eugen Hinescu6Victor Babes National Institute of Pathology, 050096 Bucharest, RomaniaVictor Babes National Institute of Pathology, 050096 Bucharest, RomaniaFundeni Clinical Institute, 022328 Bucharest, RomaniaFaculty of Medicine, Carol Davila University of Medicine and Pharmacy, 050474 Bucharest, RomaniaFaculty of Medicine, Carol Davila University of Medicine and Pharmacy, 050474 Bucharest, RomaniaVictor Babes National Institute of Pathology, 050096 Bucharest, RomaniaVictor Babes National Institute of Pathology, 050096 Bucharest, RomaniaIncreased insulin-like growth factor (IGF) axis activity is associated with the development and progression of different types of malignancies, including colorectal cancer (CRC). MicroRNAs (miRNAs) belonging to the let-7 family have been reported to target genes involved in this axis and are known as tumor suppressors. In this study, in silico bioinformatic analysis was performed to assess miRNA–mRNA interactions between eight miRNAs belonging to the let-7 family and genes involved in the IGF signaling pathway, coding for receptors and substrates. miRNAs’ expression analysis revealed that hsa-let-7a-5p, hsa-let-7b-5p, hsa-let-7c-5p, hsa-let- 97 7d-5p, hsa-let-7e-5p, hsa-let-7f-5p, and hsa-let-7g-5p were significantly down-regulated in 25 CRC tumoral tissues (T) compared to the corresponding adjacent peritumoral tissues (PT). Moreover, our results showed an upregulation of miR-let-7e-5p in CRC tissues with mutations in KRAS codon 12 or 13, and, for the first time, found a specific dysregulation of let-7a-5p, let-7b-5p, let-7c-5p, let-7d-5p, and let-7i-5p in CRC with perineural invasion. Our results sustain the relationship between the IGF axis, let-7 miRNAs, and CRC and suggest an association between the expression of these miRNAs and perineural invasion.https://www.mdpi.com/2075-1729/12/10/1638insulin-like growth factorlet-7 microRNAscolorectal cancerperineural invasion |
| spellingShingle | Andrei Marian Niculae Maria Dobre Vlad Herlea Teodora Ecaterina Manuc Bogdan Trandafir Elena Milanesi Mihail Eugen Hinescu Let-7 microRNAs Are Possibly Associated with Perineural Invasion in Colorectal Cancer by Targeting IGF Axis Life insulin-like growth factor let-7 microRNAs colorectal cancer perineural invasion |
| title | Let-7 microRNAs Are Possibly Associated with Perineural Invasion in Colorectal Cancer by Targeting IGF Axis |
| title_full | Let-7 microRNAs Are Possibly Associated with Perineural Invasion in Colorectal Cancer by Targeting IGF Axis |
| title_fullStr | Let-7 microRNAs Are Possibly Associated with Perineural Invasion in Colorectal Cancer by Targeting IGF Axis |
| title_full_unstemmed | Let-7 microRNAs Are Possibly Associated with Perineural Invasion in Colorectal Cancer by Targeting IGF Axis |
| title_short | Let-7 microRNAs Are Possibly Associated with Perineural Invasion in Colorectal Cancer by Targeting IGF Axis |
| title_sort | let 7 micrornas are possibly associated with perineural invasion in colorectal cancer by targeting igf axis |
| topic | insulin-like growth factor let-7 microRNAs colorectal cancer perineural invasion |
| url | https://www.mdpi.com/2075-1729/12/10/1638 |
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