First Report of a Methicillin-Resistant, High-Level Mupirocin-Resistant Staphylococcus argenteus

We describe the identification of a methicillin-resistant, high-level mupirocin-resistant Staphylococcus argenteus. The isolate (1801221) was characterized as t6675-ST2250-SCCmecIVc, and whole-genome sequencing revealed that the isolate possessed two plasmids. One plasmid (34,870 bp), designated p1_...

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Main Authors: Adebayo Osagie Shittu, Franziska Layer-Nicolaou, Birgit Strommenger, Minh-Thu Nguyen, Stefan Bletz, Alexander Mellmann, Frieder Schaumburg
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-03-01
Series:Frontiers in Cellular and Infection Microbiology
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fcimb.2022.860163/full
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author Adebayo Osagie Shittu
Adebayo Osagie Shittu
Franziska Layer-Nicolaou
Birgit Strommenger
Minh-Thu Nguyen
Stefan Bletz
Alexander Mellmann
Frieder Schaumburg
author_facet Adebayo Osagie Shittu
Adebayo Osagie Shittu
Franziska Layer-Nicolaou
Birgit Strommenger
Minh-Thu Nguyen
Stefan Bletz
Alexander Mellmann
Frieder Schaumburg
author_sort Adebayo Osagie Shittu
collection DOAJ
description We describe the identification of a methicillin-resistant, high-level mupirocin-resistant Staphylococcus argenteus. The isolate (1801221) was characterized as t6675-ST2250-SCCmecIVc, and whole-genome sequencing revealed that the isolate possessed two plasmids. One plasmid (34,870 bp), designated p1_1801221 with rep23, harboured the mupirocin resistance (mupA) gene. The second plasmid (20,644 bp), assigned as p2_1801221 with rep5a and rep16, carried the resistance determinants for penicillin (blaZ) and cadmium (cadD). Phylogenetic analysis revealed that the isolate clustered with the European ST2250 lineage. The overall high similarity of both plasmids in S. argenteus with published DNA sequences of Staphylococcus aureus plasmids strongly suggests an interspecies transfer. The pathogenic potential, community and nosocomial spread, and acquisition of antibiotic resistance gene determinants, including the mupA gene by S. argenteus, highlight its clinical significance and the need for its correct identification.
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spelling doaj.art-dd60a9ce76b946d5bde43cecaa662eb12022-12-21T23:17:50ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882022-03-011210.3389/fcimb.2022.860163860163First Report of a Methicillin-Resistant, High-Level Mupirocin-Resistant Staphylococcus argenteusAdebayo Osagie Shittu0Adebayo Osagie Shittu1Franziska Layer-Nicolaou2Birgit Strommenger3Minh-Thu Nguyen4Stefan Bletz5Alexander Mellmann6Frieder Schaumburg7Department of Microbiology, Obafemi Awolowo University, Ile-Ife, NigeriaInstitute of Medical Microbiology, University Hospital Münster, Münster, GermanyNational Reference Centre (NRC) for Staphylococci and Enterococci, Division of Nosocomial Pathogens and Antibiotic Resistances, Department of Infectious Diseases, Robert Koch Institute, Wernigerode Branch, Wernigerode, GermanyNational Reference Centre (NRC) for Staphylococci and Enterococci, Division of Nosocomial Pathogens and Antibiotic Resistances, Department of Infectious Diseases, Robert Koch Institute, Wernigerode Branch, Wernigerode, GermanyInstitute of Medical Microbiology, University Hospital Münster, Münster, GermanyInstitute for Hygiene, University Hospital Münster, Münster, GermanyInstitute for Hygiene, University Hospital Münster, Münster, GermanyInstitute of Medical Microbiology, University Hospital Münster, Münster, GermanyWe describe the identification of a methicillin-resistant, high-level mupirocin-resistant Staphylococcus argenteus. The isolate (1801221) was characterized as t6675-ST2250-SCCmecIVc, and whole-genome sequencing revealed that the isolate possessed two plasmids. One plasmid (34,870 bp), designated p1_1801221 with rep23, harboured the mupirocin resistance (mupA) gene. The second plasmid (20,644 bp), assigned as p2_1801221 with rep5a and rep16, carried the resistance determinants for penicillin (blaZ) and cadmium (cadD). Phylogenetic analysis revealed that the isolate clustered with the European ST2250 lineage. The overall high similarity of both plasmids in S. argenteus with published DNA sequences of Staphylococcus aureus plasmids strongly suggests an interspecies transfer. The pathogenic potential, community and nosocomial spread, and acquisition of antibiotic resistance gene determinants, including the mupA gene by S. argenteus, highlight its clinical significance and the need for its correct identification.https://www.frontiersin.org/articles/10.3389/fcimb.2022.860163/fullidentificationmethicillin-resistant Staphylococcus argenteushigh-level mupirocin resistanceplasmidwhole-genome sequencing (WGS)
spellingShingle Adebayo Osagie Shittu
Adebayo Osagie Shittu
Franziska Layer-Nicolaou
Birgit Strommenger
Minh-Thu Nguyen
Stefan Bletz
Alexander Mellmann
Frieder Schaumburg
First Report of a Methicillin-Resistant, High-Level Mupirocin-Resistant Staphylococcus argenteus
Frontiers in Cellular and Infection Microbiology
identification
methicillin-resistant Staphylococcus argenteus
high-level mupirocin resistance
plasmid
whole-genome sequencing (WGS)
title First Report of a Methicillin-Resistant, High-Level Mupirocin-Resistant Staphylococcus argenteus
title_full First Report of a Methicillin-Resistant, High-Level Mupirocin-Resistant Staphylococcus argenteus
title_fullStr First Report of a Methicillin-Resistant, High-Level Mupirocin-Resistant Staphylococcus argenteus
title_full_unstemmed First Report of a Methicillin-Resistant, High-Level Mupirocin-Resistant Staphylococcus argenteus
title_short First Report of a Methicillin-Resistant, High-Level Mupirocin-Resistant Staphylococcus argenteus
title_sort first report of a methicillin resistant high level mupirocin resistant staphylococcus argenteus
topic identification
methicillin-resistant Staphylococcus argenteus
high-level mupirocin resistance
plasmid
whole-genome sequencing (WGS)
url https://www.frontiersin.org/articles/10.3389/fcimb.2022.860163/full
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