First Report of a Methicillin-Resistant, High-Level Mupirocin-Resistant Staphylococcus argenteus
We describe the identification of a methicillin-resistant, high-level mupirocin-resistant Staphylococcus argenteus. The isolate (1801221) was characterized as t6675-ST2250-SCCmecIVc, and whole-genome sequencing revealed that the isolate possessed two plasmids. One plasmid (34,870 bp), designated p1_...
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Frontiers Media S.A.
2022-03-01
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Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2022.860163/full |
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author | Adebayo Osagie Shittu Adebayo Osagie Shittu Franziska Layer-Nicolaou Birgit Strommenger Minh-Thu Nguyen Stefan Bletz Alexander Mellmann Frieder Schaumburg |
author_facet | Adebayo Osagie Shittu Adebayo Osagie Shittu Franziska Layer-Nicolaou Birgit Strommenger Minh-Thu Nguyen Stefan Bletz Alexander Mellmann Frieder Schaumburg |
author_sort | Adebayo Osagie Shittu |
collection | DOAJ |
description | We describe the identification of a methicillin-resistant, high-level mupirocin-resistant Staphylococcus argenteus. The isolate (1801221) was characterized as t6675-ST2250-SCCmecIVc, and whole-genome sequencing revealed that the isolate possessed two plasmids. One plasmid (34,870 bp), designated p1_1801221 with rep23, harboured the mupirocin resistance (mupA) gene. The second plasmid (20,644 bp), assigned as p2_1801221 with rep5a and rep16, carried the resistance determinants for penicillin (blaZ) and cadmium (cadD). Phylogenetic analysis revealed that the isolate clustered with the European ST2250 lineage. The overall high similarity of both plasmids in S. argenteus with published DNA sequences of Staphylococcus aureus plasmids strongly suggests an interspecies transfer. The pathogenic potential, community and nosocomial spread, and acquisition of antibiotic resistance gene determinants, including the mupA gene by S. argenteus, highlight its clinical significance and the need for its correct identification. |
first_indexed | 2024-12-14T04:05:16Z |
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id | doaj.art-dd60a9ce76b946d5bde43cecaa662eb1 |
institution | Directory Open Access Journal |
issn | 2235-2988 |
language | English |
last_indexed | 2024-12-14T04:05:16Z |
publishDate | 2022-03-01 |
publisher | Frontiers Media S.A. |
record_format | Article |
series | Frontiers in Cellular and Infection Microbiology |
spelling | doaj.art-dd60a9ce76b946d5bde43cecaa662eb12022-12-21T23:17:50ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882022-03-011210.3389/fcimb.2022.860163860163First Report of a Methicillin-Resistant, High-Level Mupirocin-Resistant Staphylococcus argenteusAdebayo Osagie Shittu0Adebayo Osagie Shittu1Franziska Layer-Nicolaou2Birgit Strommenger3Minh-Thu Nguyen4Stefan Bletz5Alexander Mellmann6Frieder Schaumburg7Department of Microbiology, Obafemi Awolowo University, Ile-Ife, NigeriaInstitute of Medical Microbiology, University Hospital Münster, Münster, GermanyNational Reference Centre (NRC) for Staphylococci and Enterococci, Division of Nosocomial Pathogens and Antibiotic Resistances, Department of Infectious Diseases, Robert Koch Institute, Wernigerode Branch, Wernigerode, GermanyNational Reference Centre (NRC) for Staphylococci and Enterococci, Division of Nosocomial Pathogens and Antibiotic Resistances, Department of Infectious Diseases, Robert Koch Institute, Wernigerode Branch, Wernigerode, GermanyInstitute of Medical Microbiology, University Hospital Münster, Münster, GermanyInstitute for Hygiene, University Hospital Münster, Münster, GermanyInstitute for Hygiene, University Hospital Münster, Münster, GermanyInstitute of Medical Microbiology, University Hospital Münster, Münster, GermanyWe describe the identification of a methicillin-resistant, high-level mupirocin-resistant Staphylococcus argenteus. The isolate (1801221) was characterized as t6675-ST2250-SCCmecIVc, and whole-genome sequencing revealed that the isolate possessed two plasmids. One plasmid (34,870 bp), designated p1_1801221 with rep23, harboured the mupirocin resistance (mupA) gene. The second plasmid (20,644 bp), assigned as p2_1801221 with rep5a and rep16, carried the resistance determinants for penicillin (blaZ) and cadmium (cadD). Phylogenetic analysis revealed that the isolate clustered with the European ST2250 lineage. The overall high similarity of both plasmids in S. argenteus with published DNA sequences of Staphylococcus aureus plasmids strongly suggests an interspecies transfer. The pathogenic potential, community and nosocomial spread, and acquisition of antibiotic resistance gene determinants, including the mupA gene by S. argenteus, highlight its clinical significance and the need for its correct identification.https://www.frontiersin.org/articles/10.3389/fcimb.2022.860163/fullidentificationmethicillin-resistant Staphylococcus argenteushigh-level mupirocin resistanceplasmidwhole-genome sequencing (WGS) |
spellingShingle | Adebayo Osagie Shittu Adebayo Osagie Shittu Franziska Layer-Nicolaou Birgit Strommenger Minh-Thu Nguyen Stefan Bletz Alexander Mellmann Frieder Schaumburg First Report of a Methicillin-Resistant, High-Level Mupirocin-Resistant Staphylococcus argenteus Frontiers in Cellular and Infection Microbiology identification methicillin-resistant Staphylococcus argenteus high-level mupirocin resistance plasmid whole-genome sequencing (WGS) |
title | First Report of a Methicillin-Resistant, High-Level Mupirocin-Resistant Staphylococcus argenteus |
title_full | First Report of a Methicillin-Resistant, High-Level Mupirocin-Resistant Staphylococcus argenteus |
title_fullStr | First Report of a Methicillin-Resistant, High-Level Mupirocin-Resistant Staphylococcus argenteus |
title_full_unstemmed | First Report of a Methicillin-Resistant, High-Level Mupirocin-Resistant Staphylococcus argenteus |
title_short | First Report of a Methicillin-Resistant, High-Level Mupirocin-Resistant Staphylococcus argenteus |
title_sort | first report of a methicillin resistant high level mupirocin resistant staphylococcus argenteus |
topic | identification methicillin-resistant Staphylococcus argenteus high-level mupirocin resistance plasmid whole-genome sequencing (WGS) |
url | https://www.frontiersin.org/articles/10.3389/fcimb.2022.860163/full |
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