Long Non-coding RNA MIR4435-2HG Promotes Colorectal Cancer Proliferation and Metastasis Through miR-206/YAP1 Axis
Objective: Long non-coding RNAs (lncRNAs) are critical to colorectal cancer (CRC) progression. In the current study, the objective was the exploration of the role played by lncRNA MIR4435-2HG in CRC proliferation and metastasis.Methods: lncRNA MIR4435-2HG expression and its association with CRC were...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-02-01
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| Series: | Frontiers in Oncology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fonc.2020.00160/full |
| _version_ | 1818896917335113728 |
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| author | Xinhua Dong Zhen Yang Hongwei Yang Dongyan Li Xinguang Qiu |
| author_facet | Xinhua Dong Zhen Yang Hongwei Yang Dongyan Li Xinguang Qiu |
| author_sort | Xinhua Dong |
| collection | DOAJ |
| description | Objective: Long non-coding RNAs (lncRNAs) are critical to colorectal cancer (CRC) progression. In the current study, the objective was the exploration of the role played by lncRNA MIR4435-2HG in CRC proliferation and metastasis.Methods: lncRNA MIR4435-2HG expression and its association with CRC were analyzed using database and clinical specimens. The influences exerted by MIR4435-2HG on cell proliferating process, invading process, and migrating process of CRC were identified after MIR4435-2HG knockdown. The influences exerted by MIR4435-2HG on tumor growth and metastasis were assessed in vivo. The underlying mechanistic associations between MIR4435-2HG, microRNA miR-206, and the transcription factor Yes-associated protein 1 (YAP1) were assessed using bioinformatics and a luciferase reporter gene assay.Results: MIR4435-2HG was highly expressed in CRC tissue in contrast with that in regular tissues and displayed relations to poor prognosis. MIR4435-2HG knockdown could suppress CRC cell proliferation, invasion, and migration. Moreover, MIR4435-2HG knockdown inhibited CRC growth and liver metastasis in vitro. We found MIR4435-2HG knockdown reduced YAP1, CTGF, AREG, vimentin, Snail, Slug, and Twist expression but enhanced E-cadherin expression. Functionally, MIR4435-2HG acted as a competing endogenous RNA (ceRNA) to upregulate YAP1 by sponging miR-206.Conclusions: MIR4435-2HG promoted CRC growth and metastasis through miR-206/YAP1 axis and is likely to play prognostic marker roles and be therapeutically targeted in CRC. |
| first_indexed | 2024-12-19T19:07:54Z |
| format | Article |
| id | doaj.art-dd62f91f537a4c4e8270d0ef2bf182ba |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-12-19T19:07:54Z |
| publishDate | 2020-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-dd62f91f537a4c4e8270d0ef2bf182ba2022-12-21T20:09:23ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2020-02-011010.3389/fonc.2020.00160506140Long Non-coding RNA MIR4435-2HG Promotes Colorectal Cancer Proliferation and Metastasis Through miR-206/YAP1 AxisXinhua Dong0Zhen Yang1Hongwei Yang2Dongyan Li3Xinguang Qiu4Department of Gastroenterology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Gastroenterology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Gastroenterology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Gastroenterology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Thyroid Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaObjective: Long non-coding RNAs (lncRNAs) are critical to colorectal cancer (CRC) progression. In the current study, the objective was the exploration of the role played by lncRNA MIR4435-2HG in CRC proliferation and metastasis.Methods: lncRNA MIR4435-2HG expression and its association with CRC were analyzed using database and clinical specimens. The influences exerted by MIR4435-2HG on cell proliferating process, invading process, and migrating process of CRC were identified after MIR4435-2HG knockdown. The influences exerted by MIR4435-2HG on tumor growth and metastasis were assessed in vivo. The underlying mechanistic associations between MIR4435-2HG, microRNA miR-206, and the transcription factor Yes-associated protein 1 (YAP1) were assessed using bioinformatics and a luciferase reporter gene assay.Results: MIR4435-2HG was highly expressed in CRC tissue in contrast with that in regular tissues and displayed relations to poor prognosis. MIR4435-2HG knockdown could suppress CRC cell proliferation, invasion, and migration. Moreover, MIR4435-2HG knockdown inhibited CRC growth and liver metastasis in vitro. We found MIR4435-2HG knockdown reduced YAP1, CTGF, AREG, vimentin, Snail, Slug, and Twist expression but enhanced E-cadherin expression. Functionally, MIR4435-2HG acted as a competing endogenous RNA (ceRNA) to upregulate YAP1 by sponging miR-206.Conclusions: MIR4435-2HG promoted CRC growth and metastasis through miR-206/YAP1 axis and is likely to play prognostic marker roles and be therapeutically targeted in CRC.https://www.frontiersin.org/article/10.3389/fonc.2020.00160/fullcolorectal cancerlong non-coding RNAMIR4435-2HGproliferationmetastasisYAP1 |
| spellingShingle | Xinhua Dong Zhen Yang Hongwei Yang Dongyan Li Xinguang Qiu Long Non-coding RNA MIR4435-2HG Promotes Colorectal Cancer Proliferation and Metastasis Through miR-206/YAP1 Axis Frontiers in Oncology colorectal cancer long non-coding RNA MIR4435-2HG proliferation metastasis YAP1 |
| title | Long Non-coding RNA MIR4435-2HG Promotes Colorectal Cancer Proliferation and Metastasis Through miR-206/YAP1 Axis |
| title_full | Long Non-coding RNA MIR4435-2HG Promotes Colorectal Cancer Proliferation and Metastasis Through miR-206/YAP1 Axis |
| title_fullStr | Long Non-coding RNA MIR4435-2HG Promotes Colorectal Cancer Proliferation and Metastasis Through miR-206/YAP1 Axis |
| title_full_unstemmed | Long Non-coding RNA MIR4435-2HG Promotes Colorectal Cancer Proliferation and Metastasis Through miR-206/YAP1 Axis |
| title_short | Long Non-coding RNA MIR4435-2HG Promotes Colorectal Cancer Proliferation and Metastasis Through miR-206/YAP1 Axis |
| title_sort | long non coding rna mir4435 2hg promotes colorectal cancer proliferation and metastasis through mir 206 yap1 axis |
| topic | colorectal cancer long non-coding RNA MIR4435-2HG proliferation metastasis YAP1 |
| url | https://www.frontiersin.org/article/10.3389/fonc.2020.00160/full |
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