Serum thyroglobulin evaluation on LC-MS/MS and immunoassay in TgAb-positive patients with papillary thyroid carcinoma
Objective: This study aimed to elucidate disproportionately low serum thyroglobulin (Tg) values in Tg antibody (TgAb)-positive patients with structural recurrence of papillary thyroid carcinoma (PTC) using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Design: A retrospective study was...
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| Format: | Article |
| Language: | English |
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Bioscientifica
2023-01-01
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| Series: | European Thyroid Journal |
| Subjects: | |
| Online Access: | https://etj.bioscientifica.com/view/journals/etj/11/1/ETJ-21-0041.xml |
| _version_ | 1797955241677684736 |
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| author | Eijun Nishihara Yoshitaka Hobo Akira Miyauchi Yasuhiro Ito Miyoko Higuchi Mitsuyoshi Hirokawa Mitsuru Ito Shuji Fukata Mitsushige Nishikawa Takashi Akamizu |
| author_facet | Eijun Nishihara Yoshitaka Hobo Akira Miyauchi Yasuhiro Ito Miyoko Higuchi Mitsuyoshi Hirokawa Mitsuru Ito Shuji Fukata Mitsushige Nishikawa Takashi Akamizu |
| author_sort | Eijun Nishihara |
| collection | DOAJ |
| description | Objective: This study aimed to elucidate disproportionately low serum thyroglobulin (Tg) values in Tg antibody (TgAb)-positive patients with structural recurrence of papillary thyroid carcinoma (PTC) using liquid chromatography-tandem mass spectrometry (LC-MS/MS).
Design: A retrospective study was performed on 176 patients in whom Tg and TgAb levels were measured between 2016 and 2021. Several comprehensive analyses of Tg-LC-MS/ MS with an electrochemiluminescence immunoassay for Tg (Tg-ECLIA) were conducted using serum samples.
Methods: TgAb-positive patients who underwent total thyroidectomy with multiple lung metastases due to PTC were evaluated using Tg-LC-MS/MS and Tg-ECLIA. Tg expression in lymph node metastases and metastatic lesions was evaluated by immunohistochemistry and Tg levels of aspiration washouts were also evaluated. Two in vitro assays were performed to elucidate TgAb interference.
Results: Tg concentrations of negative TgAb in both assays were similar (R2 = 0.99; n = 52). Patients with structural recurrence showed higher Tg values with Tg-LC-MS/MS than with Tg-ECLIA. The undetectable proportion was significantly lower wi th Tg-LC-MS/MS (31.6%, 6/19) than with Tg-ECLIA (68.4%, 13/19; P = 0.023). The spike-recovery rate and Tg concentrations determined by the serum mixture text (n = 29) were significantly reduced to 75.0% (118.3–88.7%) and 81.3% (107.0–87.0%), respectively, w ith TgAb using Tg-ECLIA (both P < 0.001) confirming assay interference but not using Tg-LC-MS/ MS (91.8–92.3%, P = 0.77 and 98.4–100.8%, P = 0.18, respectively).
Conclusions: TgAb had no effect on the Tg-LC-MS/MS assay but yielded 19–25% l ower values in Tg-ECLIA. Tg-LC-MS/MS is preferable for monitoring serum Tg levels in TgAb-positive patients, although those with structural recurrence often had disproportionally low Tg values. |
| first_indexed | 2024-04-10T23:31:08Z |
| format | Article |
| id | doaj.art-dd633f733f7b42fabee1c226908a78ea |
| institution | Directory Open Access Journal |
| issn | 2235-0802 |
| language | English |
| last_indexed | 2024-04-10T23:31:08Z |
| publishDate | 2023-01-01 |
| publisher | Bioscientifica |
| record_format | Article |
| series | European Thyroid Journal |
| spelling | doaj.art-dd633f733f7b42fabee1c226908a78ea2023-01-12T06:58:09ZengBioscientificaEuropean Thyroid Journal2235-08022023-01-0111118https://doi.org/10.1530/ETJ-21-0041Serum thyroglobulin evaluation on LC-MS/MS and immunoassay in TgAb-positive patients with papillary thyroid carcinomaEijun Nishihara0Yoshitaka Hobo1Akira Miyauchi2Yasuhiro Ito3Miyoko Higuchi4Mitsuyoshi Hirokawa5Mitsuru Ito6Shuji Fukata7Mitsushige Nishikawa8Takashi Akamizu9Kuma Hospital, Center for Excellence in Thyroid Care, Kobe, JapanASKA Pharmamedical Co., Ltd. Fujisawa, JapanKuma Hospital, Center for Excellence in Thyroid Care, Kobe, JapanKuma Hospital, Center for Excellence in Thyroid Care, Kobe, JapanKuma Hospital, Center for Excellence in Thyroid Care, Kobe, JapanKuma Hospital, Center for Excellence in Thyroid Care, Kobe, JapanKuma Hospital, Center for Excellence in Thyroid Care, Kobe, JapanKuma Hospital, Center for Excellence in Thyroid Care, Kobe, JapanKuma Hospital, Center for Excellence in Thyroid Care, Kobe, JapanKuma Hospital, Center for Excellence in Thyroid Care, Kobe, JapanObjective: This study aimed to elucidate disproportionately low serum thyroglobulin (Tg) values in Tg antibody (TgAb)-positive patients with structural recurrence of papillary thyroid carcinoma (PTC) using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Design: A retrospective study was performed on 176 patients in whom Tg and TgAb levels were measured between 2016 and 2021. Several comprehensive analyses of Tg-LC-MS/ MS with an electrochemiluminescence immunoassay for Tg (Tg-ECLIA) were conducted using serum samples. Methods: TgAb-positive patients who underwent total thyroidectomy with multiple lung metastases due to PTC were evaluated using Tg-LC-MS/MS and Tg-ECLIA. Tg expression in lymph node metastases and metastatic lesions was evaluated by immunohistochemistry and Tg levels of aspiration washouts were also evaluated. Two in vitro assays were performed to elucidate TgAb interference. Results: Tg concentrations of negative TgAb in both assays were similar (R2 = 0.99; n = 52). Patients with structural recurrence showed higher Tg values with Tg-LC-MS/MS than with Tg-ECLIA. The undetectable proportion was significantly lower wi th Tg-LC-MS/MS (31.6%, 6/19) than with Tg-ECLIA (68.4%, 13/19; P = 0.023). The spike-recovery rate and Tg concentrations determined by the serum mixture text (n = 29) were significantly reduced to 75.0% (118.3–88.7%) and 81.3% (107.0–87.0%), respectively, w ith TgAb using Tg-ECLIA (both P < 0.001) confirming assay interference but not using Tg-LC-MS/ MS (91.8–92.3%, P = 0.77 and 98.4–100.8%, P = 0.18, respectively). Conclusions: TgAb had no effect on the Tg-LC-MS/MS assay but yielded 19–25% l ower values in Tg-ECLIA. Tg-LC-MS/MS is preferable for monitoring serum Tg levels in TgAb-positive patients, although those with structural recurrence often had disproportionally low Tg values.https://etj.bioscientifica.com/view/journals/etj/11/1/ETJ-21-0041.xmlserum thyroglobulininterference with tgablc-ms/msstructural recurrence |
| spellingShingle | Eijun Nishihara Yoshitaka Hobo Akira Miyauchi Yasuhiro Ito Miyoko Higuchi Mitsuyoshi Hirokawa Mitsuru Ito Shuji Fukata Mitsushige Nishikawa Takashi Akamizu Serum thyroglobulin evaluation on LC-MS/MS and immunoassay in TgAb-positive patients with papillary thyroid carcinoma European Thyroid Journal serum thyroglobulin interference with tgab lc-ms/ms structural recurrence |
| title | Serum thyroglobulin evaluation on LC-MS/MS and immunoassay in TgAb-positive patients with papillary thyroid carcinoma |
| title_full | Serum thyroglobulin evaluation on LC-MS/MS and immunoassay in TgAb-positive patients with papillary thyroid carcinoma |
| title_fullStr | Serum thyroglobulin evaluation on LC-MS/MS and immunoassay in TgAb-positive patients with papillary thyroid carcinoma |
| title_full_unstemmed | Serum thyroglobulin evaluation on LC-MS/MS and immunoassay in TgAb-positive patients with papillary thyroid carcinoma |
| title_short | Serum thyroglobulin evaluation on LC-MS/MS and immunoassay in TgAb-positive patients with papillary thyroid carcinoma |
| title_sort | serum thyroglobulin evaluation on lc ms ms and immunoassay in tgab positive patients with papillary thyroid carcinoma |
| topic | serum thyroglobulin interference with tgab lc-ms/ms structural recurrence |
| url | https://etj.bioscientifica.com/view/journals/etj/11/1/ETJ-21-0041.xml |
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