GENETIC AND ANTIGENIC CHARACTERISTICS OF RESPIRATORY SYNCYTIAL VIRUS STRAINS ISOLATED IN ST. PETERSBURG IN 2013-2016
Antigenic and genetic characteristics of Russian RSV isolates are presented for the first time. Of the 69 strains isolated in St. Petersburg, 93% belonged to the RSV-A antigenic group. The antigenic variations in the F-protein RSV were analyzed using a panel from 6 monoclonal antibodies by the metho...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Central Research Institute for Epidemiology
2017-12-01
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| Series: | Вопросы вирусологии |
| Subjects: | |
| Online Access: | https://virusjour.crie.ru/jour/article/viewFile/175/85 |
| _version_ | 1827903097350914048 |
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| author | V. Z. Krivitskaya K. S. Sintsova E. R. Petrova M. V. Sverlova E. V. Sorokin T. R. Tsareva A. B. Komissarov A. V. Fadeev M. M. Pisareva Zh. V. Buzitskaya V. S. Afanaseva V. F. Sukhovetskaya A. A. Sominina |
| author_facet | V. Z. Krivitskaya K. S. Sintsova E. R. Petrova M. V. Sverlova E. V. Sorokin T. R. Tsareva A. B. Komissarov A. V. Fadeev M. M. Pisareva Zh. V. Buzitskaya V. S. Afanaseva V. F. Sukhovetskaya A. A. Sominina |
| author_sort | V. Z. Krivitskaya |
| collection | DOAJ |
| description | Antigenic and genetic characteristics of Russian RSV isolates are presented for the first time. Of the 69 strains isolated in St. Petersburg, 93% belonged to the RSV-A antigenic group. The antigenic variations in the F-protein RSV were analyzed using a panel from 6 monoclonal antibodies by the method of micro-cultural ELISA. Depending on the decrease in the effectiveness of interaction with monoclonal antibodies (relative to the reference strain Long), RSV-A isolates were divided into 4 antigenic subgroups. The results of 24 isolates sequencing showed that more than 60% of them had substitutions in significant F-protein sites compared to the ON67-1210A reference strain of the current RSV genotype ON1/GA2. The most variable were the signal peptide and antigenic site II. When comparing the results of ELISA and sequencing, it was not possible to identify any specific key substitutions in the amino acid sequence of the F-protein that affect the interaction of the virus with antibodies. The nucleotide sequence of the F-gene from 19 of the 24 characterized isolates was close to that of ON67-1210A reference virus and was significantly different from RSV-A Long and A2 viruses. A separate group consisted of 5 strains, in which the F-protein structure was approximated to RSV Long. |
| first_indexed | 2024-03-13T00:08:10Z |
| format | Article |
| id | doaj.art-dd6384523d804ce18bc2b12307b346e2 |
| institution | Directory Open Access Journal |
| issn | 0507-4088 2411-2097 |
| language | English |
| last_indexed | 2024-03-13T00:08:10Z |
| publishDate | 2017-12-01 |
| publisher | Central Research Institute for Epidemiology |
| record_format | Article |
| series | Вопросы вирусологии |
| spelling | doaj.art-dd6384523d804ce18bc2b12307b346e22023-07-12T20:02:03ZengCentral Research Institute for EpidemiologyВопросы вирусологии0507-40882411-20972017-12-0162627328210.18821/0507-4088-2017-62-6-273-282155GENETIC AND ANTIGENIC CHARACTERISTICS OF RESPIRATORY SYNCYTIAL VIRUS STRAINS ISOLATED IN ST. PETERSBURG IN 2013-2016V. Z. Krivitskaya0K. S. Sintsova1E. R. Petrova2M. V. Sverlova3E. V. Sorokin4T. R. Tsareva5A. B. Komissarov6A. V. Fadeev7M. M. Pisareva8Zh. V. Buzitskaya9V. S. Afanaseva10V. F. Sukhovetskaya11A. A. Sominina12Research Institute of InfluenzaResearch Institute of InfluenzaResearch Institute of InfluenzaResearch Institute of InfluenzaResearch Institute of InfluenzaResearch Institute of InfluenzaResearch Institute of InfluenzaResearch Institute of InfluenzaResearch Institute of InfluenzaResearch Institute of InfluenzaResearch Institute of InfluenzaResearch Institute of InfluenzaResearch Institute of InfluenzaAntigenic and genetic characteristics of Russian RSV isolates are presented for the first time. Of the 69 strains isolated in St. Petersburg, 93% belonged to the RSV-A antigenic group. The antigenic variations in the F-protein RSV were analyzed using a panel from 6 monoclonal antibodies by the method of micro-cultural ELISA. Depending on the decrease in the effectiveness of interaction with monoclonal antibodies (relative to the reference strain Long), RSV-A isolates were divided into 4 antigenic subgroups. The results of 24 isolates sequencing showed that more than 60% of them had substitutions in significant F-protein sites compared to the ON67-1210A reference strain of the current RSV genotype ON1/GA2. The most variable were the signal peptide and antigenic site II. When comparing the results of ELISA and sequencing, it was not possible to identify any specific key substitutions in the amino acid sequence of the F-protein that affect the interaction of the virus with antibodies. The nucleotide sequence of the F-gene from 19 of the 24 characterized isolates was close to that of ON67-1210A reference virus and was significantly different from RSV-A Long and A2 viruses. A separate group consisted of 5 strains, in which the F-protein structure was approximated to RSV Long.https://virusjour.crie.ru/jour/article/viewFile/175/85f-белокrespiratory syncytial virusf-proteinphylogenetic analysis |
| spellingShingle | V. Z. Krivitskaya K. S. Sintsova E. R. Petrova M. V. Sverlova E. V. Sorokin T. R. Tsareva A. B. Komissarov A. V. Fadeev M. M. Pisareva Zh. V. Buzitskaya V. S. Afanaseva V. F. Sukhovetskaya A. A. Sominina GENETIC AND ANTIGENIC CHARACTERISTICS OF RESPIRATORY SYNCYTIAL VIRUS STRAINS ISOLATED IN ST. PETERSBURG IN 2013-2016 Вопросы вирусологии f-белок respiratory syncytial virus f-protein phylogenetic analysis |
| title | GENETIC AND ANTIGENIC CHARACTERISTICS OF RESPIRATORY SYNCYTIAL VIRUS STRAINS ISOLATED IN ST. PETERSBURG IN 2013-2016 |
| title_full | GENETIC AND ANTIGENIC CHARACTERISTICS OF RESPIRATORY SYNCYTIAL VIRUS STRAINS ISOLATED IN ST. PETERSBURG IN 2013-2016 |
| title_fullStr | GENETIC AND ANTIGENIC CHARACTERISTICS OF RESPIRATORY SYNCYTIAL VIRUS STRAINS ISOLATED IN ST. PETERSBURG IN 2013-2016 |
| title_full_unstemmed | GENETIC AND ANTIGENIC CHARACTERISTICS OF RESPIRATORY SYNCYTIAL VIRUS STRAINS ISOLATED IN ST. PETERSBURG IN 2013-2016 |
| title_short | GENETIC AND ANTIGENIC CHARACTERISTICS OF RESPIRATORY SYNCYTIAL VIRUS STRAINS ISOLATED IN ST. PETERSBURG IN 2013-2016 |
| title_sort | genetic and antigenic characteristics of respiratory syncytial virus strains isolated in st petersburg in 2013 2016 |
| topic | f-белок respiratory syncytial virus f-protein phylogenetic analysis |
| url | https://virusjour.crie.ru/jour/article/viewFile/175/85 |
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