Increases in alginate production and transcription levels of alginate lyase (alyA1) by control of the oxygen transfer rate in Azotobacter vinelandii cultures under diazotrophic conditions
Background: Alginates are polysaccharides used in a wide range of industrial applications, with their functional properties depending on their molecular weight. In this study, alginate production and the expression of genes involved in polymerization and depolymerization in batch cultures of Azotoba...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-07-01
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| Series: | Electronic Journal of Biotechnology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0717345821000221 |
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| author | Belén Ponce Viviana Urtuvia Nataly Maturana Carlos Peña Alvaro Díaz-Barrera |
| author_facet | Belén Ponce Viviana Urtuvia Nataly Maturana Carlos Peña Alvaro Díaz-Barrera |
| author_sort | Belén Ponce |
| collection | DOAJ |
| description | Background: Alginates are polysaccharides used in a wide range of industrial applications, with their functional properties depending on their molecular weight. In this study, alginate production and the expression of genes involved in polymerization and depolymerization in batch cultures of Azotobacter vinelandii were evaluated under controlled and noncontrolled oxygen transfer rate (OTR) conditions. Results: Using an oxygen transfer rate (OTR) control system, a constant OTR (20.3 ± 1.3 mmol L−1h−1) was maintained during cell growth and stationary phases. In cultures subjected to a controlled OTR, alginate concentrations were higher (5.5 ± 0.2 g L−1) than in cultures under noncontrolled OTR. The molecular weight of alginate decreased from 475 to 325 kDa at the beginning of the growth phase and remained constant until the end of the cultivation period. The expression level of alyA1, which encodes an alginate lyase, was more affected by OTR control than those of other genes involved in alginate biosynthesis. The decrease in alginate molecular weight can be explained by a higher relative expression level of alyA1 under the controlled OTR condition. Conclusions: This report describes the first time that alginate production and alginate lyase (alyA1) expression levels have been evaluated in A. vinelandii cultures subjected to a controlled OTR. The results show that automatic control of OTR may be a suitable strategy for improving alginate production while maintaining a constant molecular weight.How to cite: Ponce B, Urtuvia V, Maturana N, et al. Increases in alginate production and transcription levels of alginate lyase (alyA1) by control of the oxygen transfer rate in Azotobacter vinelandii cultures under diazotrophic conditions. Electron J Biotechnol 2021;51. https://doi.org/10.1016/j.ejbt.2021.04.007 |
| first_indexed | 2024-12-22T01:01:20Z |
| format | Article |
| id | doaj.art-dd63be20fa1949e8bb3dffeaa75519da |
| institution | Directory Open Access Journal |
| issn | 0717-3458 |
| language | English |
| last_indexed | 2024-12-22T01:01:20Z |
| publishDate | 2021-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Electronic Journal of Biotechnology |
| spelling | doaj.art-dd63be20fa1949e8bb3dffeaa75519da2022-12-21T18:44:11ZengElsevierElectronic Journal of Biotechnology0717-34582021-07-01523544Increases in alginate production and transcription levels of alginate lyase (alyA1) by control of the oxygen transfer rate in Azotobacter vinelandii cultures under diazotrophic conditionsBelén Ponce0Viviana Urtuvia1Nataly Maturana2Carlos Peña3Alvaro Díaz-Barrera4Escuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaíso, Av. Brasil 2147 Casilla 4059, Valparaíso, ChileEscuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaíso, Av. Brasil 2147 Casilla 4059, Valparaíso, ChileEscuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaíso, Av. Brasil 2147 Casilla 4059, Valparaíso, ChileDepartamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, MexicoEscuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaíso, Av. Brasil 2147 Casilla 4059, Valparaíso, Chile; Corresponding author.Background: Alginates are polysaccharides used in a wide range of industrial applications, with their functional properties depending on their molecular weight. In this study, alginate production and the expression of genes involved in polymerization and depolymerization in batch cultures of Azotobacter vinelandii were evaluated under controlled and noncontrolled oxygen transfer rate (OTR) conditions. Results: Using an oxygen transfer rate (OTR) control system, a constant OTR (20.3 ± 1.3 mmol L−1h−1) was maintained during cell growth and stationary phases. In cultures subjected to a controlled OTR, alginate concentrations were higher (5.5 ± 0.2 g L−1) than in cultures under noncontrolled OTR. The molecular weight of alginate decreased from 475 to 325 kDa at the beginning of the growth phase and remained constant until the end of the cultivation period. The expression level of alyA1, which encodes an alginate lyase, was more affected by OTR control than those of other genes involved in alginate biosynthesis. The decrease in alginate molecular weight can be explained by a higher relative expression level of alyA1 under the controlled OTR condition. Conclusions: This report describes the first time that alginate production and alginate lyase (alyA1) expression levels have been evaluated in A. vinelandii cultures subjected to a controlled OTR. The results show that automatic control of OTR may be a suitable strategy for improving alginate production while maintaining a constant molecular weight.How to cite: Ponce B, Urtuvia V, Maturana N, et al. Increases in alginate production and transcription levels of alginate lyase (alyA1) by control of the oxygen transfer rate in Azotobacter vinelandii cultures under diazotrophic conditions. Electron J Biotechnol 2021;51. https://doi.org/10.1016/j.ejbt.2021.04.007http://www.sciencedirect.com/science/article/pii/S0717345821000221Alginate lyaseAlginateAzotobacter vinelandiiGene expressionOxygen transfer ratePolysaccharides |
| spellingShingle | Belén Ponce Viviana Urtuvia Nataly Maturana Carlos Peña Alvaro Díaz-Barrera Increases in alginate production and transcription levels of alginate lyase (alyA1) by control of the oxygen transfer rate in Azotobacter vinelandii cultures under diazotrophic conditions Electronic Journal of Biotechnology Alginate lyase Alginate Azotobacter vinelandii Gene expression Oxygen transfer rate Polysaccharides |
| title | Increases in alginate production and transcription levels of alginate lyase (alyA1) by control of the oxygen transfer rate in Azotobacter vinelandii cultures under diazotrophic conditions |
| title_full | Increases in alginate production and transcription levels of alginate lyase (alyA1) by control of the oxygen transfer rate in Azotobacter vinelandii cultures under diazotrophic conditions |
| title_fullStr | Increases in alginate production and transcription levels of alginate lyase (alyA1) by control of the oxygen transfer rate in Azotobacter vinelandii cultures under diazotrophic conditions |
| title_full_unstemmed | Increases in alginate production and transcription levels of alginate lyase (alyA1) by control of the oxygen transfer rate in Azotobacter vinelandii cultures under diazotrophic conditions |
| title_short | Increases in alginate production and transcription levels of alginate lyase (alyA1) by control of the oxygen transfer rate in Azotobacter vinelandii cultures under diazotrophic conditions |
| title_sort | increases in alginate production and transcription levels of alginate lyase alya1 by control of the oxygen transfer rate in azotobacter vinelandii cultures under diazotrophic conditions |
| topic | Alginate lyase Alginate Azotobacter vinelandii Gene expression Oxygen transfer rate Polysaccharides |
| url | http://www.sciencedirect.com/science/article/pii/S0717345821000221 |
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