Enhancement of Enzymatic Hydrolysis of Sugarcane Bagasse by the Combination of Delignification Pretreatment and Polysorbate 80
Delignification pretreatment with alkali under various conditions (25–160 °C for 1–12 h) or sodium chlorite at 75 °C for 4 h was applied to improve the enzymatic digestibility of sugarcane bagasse by removing hemicellulose and lignin. Compared with the elimination of hemicellulose, delignification c...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-04-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/9/4/371 |
| _version_ | 1797605538735849472 |
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| author | Hongdan Zhang Tao Dai Shihang Huang Jun Xie |
| author_facet | Hongdan Zhang Tao Dai Shihang Huang Jun Xie |
| author_sort | Hongdan Zhang |
| collection | DOAJ |
| description | Delignification pretreatment with alkali under various conditions (25–160 °C for 1–12 h) or sodium chlorite at 75 °C for 4 h was applied to improve the enzymatic digestibility of sugarcane bagasse by removing hemicellulose and lignin. Compared with the elimination of hemicellulose, delignification contributed more in achieving a higher glucose yield. In addition, the characterization of untreated and pretreated sugarcane bagasse was conducted to determine the influence of hemicellulose and lignin degradation on subsequent enzymatic digestibility. Furthermore, Polysorbate 80 was added to reduce the enzyme loading, shorten the hydrolysis time, and enhance the efficiency of enzymatic hydrolysis, suggesting that the glucose yield of 92.2% was obtained with enzyme loading of 5 FPU/g substrate. However, the increased yield of glucose with Polysorbate 80 occurred with an increased lignin content and a reduction of enzyme loading, and the yield decreased sharply as the hydrolysis time was prolonged from 6 h to 24 h. |
| first_indexed | 2024-03-11T05:02:31Z |
| format | Article |
| id | doaj.art-dd644cfaa27747cab549f9c2409049dd |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-11T05:02:31Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-dd644cfaa27747cab549f9c2409049dd2023-11-17T19:10:58ZengMDPI AGFermentation2311-56372023-04-019437110.3390/fermentation9040371Enhancement of Enzymatic Hydrolysis of Sugarcane Bagasse by the Combination of Delignification Pretreatment and Polysorbate 80Hongdan Zhang0Tao Dai1Shihang Huang2Jun Xie3Key Laboratory of Energy Plants Resource and Utilization, Institute of Biomass Engineering, Ministry of Agriculture and Rural Affairs, South China Agricultural University, Guangzhou 510642, ChinaKey Laboratory of Energy Plants Resource and Utilization, Institute of Biomass Engineering, Ministry of Agriculture and Rural Affairs, South China Agricultural University, Guangzhou 510642, ChinaKey Laboratory of Energy Plants Resource and Utilization, Institute of Biomass Engineering, Ministry of Agriculture and Rural Affairs, South China Agricultural University, Guangzhou 510642, ChinaKey Laboratory of Energy Plants Resource and Utilization, Institute of Biomass Engineering, Ministry of Agriculture and Rural Affairs, South China Agricultural University, Guangzhou 510642, ChinaDelignification pretreatment with alkali under various conditions (25–160 °C for 1–12 h) or sodium chlorite at 75 °C for 4 h was applied to improve the enzymatic digestibility of sugarcane bagasse by removing hemicellulose and lignin. Compared with the elimination of hemicellulose, delignification contributed more in achieving a higher glucose yield. In addition, the characterization of untreated and pretreated sugarcane bagasse was conducted to determine the influence of hemicellulose and lignin degradation on subsequent enzymatic digestibility. Furthermore, Polysorbate 80 was added to reduce the enzyme loading, shorten the hydrolysis time, and enhance the efficiency of enzymatic hydrolysis, suggesting that the glucose yield of 92.2% was obtained with enzyme loading of 5 FPU/g substrate. However, the increased yield of glucose with Polysorbate 80 occurred with an increased lignin content and a reduction of enzyme loading, and the yield decreased sharply as the hydrolysis time was prolonged from 6 h to 24 h.https://www.mdpi.com/2311-5637/9/4/371sugarcane bagassealkaline pretreatmentsodium chlorite pretreatmentglucosePolysorbate 80 |
| spellingShingle | Hongdan Zhang Tao Dai Shihang Huang Jun Xie Enhancement of Enzymatic Hydrolysis of Sugarcane Bagasse by the Combination of Delignification Pretreatment and Polysorbate 80 Fermentation sugarcane bagasse alkaline pretreatment sodium chlorite pretreatment glucose Polysorbate 80 |
| title | Enhancement of Enzymatic Hydrolysis of Sugarcane Bagasse by the Combination of Delignification Pretreatment and Polysorbate 80 |
| title_full | Enhancement of Enzymatic Hydrolysis of Sugarcane Bagasse by the Combination of Delignification Pretreatment and Polysorbate 80 |
| title_fullStr | Enhancement of Enzymatic Hydrolysis of Sugarcane Bagasse by the Combination of Delignification Pretreatment and Polysorbate 80 |
| title_full_unstemmed | Enhancement of Enzymatic Hydrolysis of Sugarcane Bagasse by the Combination of Delignification Pretreatment and Polysorbate 80 |
| title_short | Enhancement of Enzymatic Hydrolysis of Sugarcane Bagasse by the Combination of Delignification Pretreatment and Polysorbate 80 |
| title_sort | enhancement of enzymatic hydrolysis of sugarcane bagasse by the combination of delignification pretreatment and polysorbate 80 |
| topic | sugarcane bagasse alkaline pretreatment sodium chlorite pretreatment glucose Polysorbate 80 |
| url | https://www.mdpi.com/2311-5637/9/4/371 |
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