Quorum Quenching Activity of the PGPR Bacillus subtilis UD1022 Alters Nodulation Efficiency of Sinorhizobium meliloti on Medicago truncatula
Plant growth-promoting rhizobacteria (PGPR) have enormous potential for solving some of the myriad challenges facing our global agricultural system. Intense research efforts are rapidly moving the field forward and illuminating the wide diversity of bacteria and their plant beneficial activities. In...
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| Format: | Article |
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Frontiers Media S.A.
2021-01-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2020.596299/full |
| _version_ | 1818605700184539136 |
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| author | Amanda Rosier Amanda Rosier Pascale B. Beauregard Harsh P. Bais Harsh P. Bais |
| author_facet | Amanda Rosier Amanda Rosier Pascale B. Beauregard Harsh P. Bais Harsh P. Bais |
| author_sort | Amanda Rosier |
| collection | DOAJ |
| description | Plant growth-promoting rhizobacteria (PGPR) have enormous potential for solving some of the myriad challenges facing our global agricultural system. Intense research efforts are rapidly moving the field forward and illuminating the wide diversity of bacteria and their plant beneficial activities. In the development of better crop solutions using these PGPR, producers are including multiple different species of PGPR in their formulations in a “consortia” approach. While the intention is to emulate more natural rhizomicrobiome systems, the aspect of bacterial interactions has not been properly regarded. By using a tri-trophic model of Medicago truncatula A17 Jemalong, its nitrogen (N)-fixing symbiont Sinorhizobium meliloti Rm8530, and the PGPR Bacillus subtilis UD1022, we demonstrate indirect influences between the bacteria affecting their plant growth-promoting activities. Co-cultures of UD1022 with Rm8530 significantly reduced Rm8530 biofilm formation and downregulated quorum sensing (QS) genes responsible for symbiotically active biofilm production. This work also identifies the presence and activity of a quorum quenching lactonase in UD1022 and proposes this as the mechanism for non-synergistic activity of this model “consortium.” These interspecies interactions may be common in the rhizosphere and are critical to understand as we seek to develop new sustainable solutions in agriculture. |
| first_indexed | 2024-12-16T13:59:07Z |
| format | Article |
| id | doaj.art-dd6a5df1be784a988ae4ee3ee3adaf8a |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-16T13:59:07Z |
| publishDate | 2021-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-dd6a5df1be784a988ae4ee3ee3adaf8a2022-12-21T22:29:09ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-01-011110.3389/fmicb.2020.596299596299Quorum Quenching Activity of the PGPR Bacillus subtilis UD1022 Alters Nodulation Efficiency of Sinorhizobium meliloti on Medicago truncatulaAmanda Rosier0Amanda Rosier1Pascale B. Beauregard2Harsh P. Bais3Harsh P. Bais4Department of Plant and Soil Sciences, University of Delaware, Newark, DE, United StatesDelaware Biotechnology Institute, University of Delaware, Newark, DE, United StatesDépartement de Biologie, Université de Sherbrooke, Sherbrooke, QC, CanadaDepartment of Plant and Soil Sciences, University of Delaware, Newark, DE, United StatesDelaware Biotechnology Institute, University of Delaware, Newark, DE, United StatesPlant growth-promoting rhizobacteria (PGPR) have enormous potential for solving some of the myriad challenges facing our global agricultural system. Intense research efforts are rapidly moving the field forward and illuminating the wide diversity of bacteria and their plant beneficial activities. In the development of better crop solutions using these PGPR, producers are including multiple different species of PGPR in their formulations in a “consortia” approach. While the intention is to emulate more natural rhizomicrobiome systems, the aspect of bacterial interactions has not been properly regarded. By using a tri-trophic model of Medicago truncatula A17 Jemalong, its nitrogen (N)-fixing symbiont Sinorhizobium meliloti Rm8530, and the PGPR Bacillus subtilis UD1022, we demonstrate indirect influences between the bacteria affecting their plant growth-promoting activities. Co-cultures of UD1022 with Rm8530 significantly reduced Rm8530 biofilm formation and downregulated quorum sensing (QS) genes responsible for symbiotically active biofilm production. This work also identifies the presence and activity of a quorum quenching lactonase in UD1022 and proposes this as the mechanism for non-synergistic activity of this model “consortium.” These interspecies interactions may be common in the rhizosphere and are critical to understand as we seek to develop new sustainable solutions in agriculture.https://www.frontiersin.org/articles/10.3389/fmicb.2020.596299/fullPGPRsymbiosisconsortialegumequorum sensingquorum quenching |
| spellingShingle | Amanda Rosier Amanda Rosier Pascale B. Beauregard Harsh P. Bais Harsh P. Bais Quorum Quenching Activity of the PGPR Bacillus subtilis UD1022 Alters Nodulation Efficiency of Sinorhizobium meliloti on Medicago truncatula Frontiers in Microbiology PGPR symbiosis consortia legume quorum sensing quorum quenching |
| title | Quorum Quenching Activity of the PGPR Bacillus subtilis UD1022 Alters Nodulation Efficiency of Sinorhizobium meliloti on Medicago truncatula |
| title_full | Quorum Quenching Activity of the PGPR Bacillus subtilis UD1022 Alters Nodulation Efficiency of Sinorhizobium meliloti on Medicago truncatula |
| title_fullStr | Quorum Quenching Activity of the PGPR Bacillus subtilis UD1022 Alters Nodulation Efficiency of Sinorhizobium meliloti on Medicago truncatula |
| title_full_unstemmed | Quorum Quenching Activity of the PGPR Bacillus subtilis UD1022 Alters Nodulation Efficiency of Sinorhizobium meliloti on Medicago truncatula |
| title_short | Quorum Quenching Activity of the PGPR Bacillus subtilis UD1022 Alters Nodulation Efficiency of Sinorhizobium meliloti on Medicago truncatula |
| title_sort | quorum quenching activity of the pgpr bacillus subtilis ud1022 alters nodulation efficiency of sinorhizobium meliloti on medicago truncatula |
| topic | PGPR symbiosis consortia legume quorum sensing quorum quenching |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2020.596299/full |
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