<i>Ribes nigrum</i> L. Extract-Mediated Green Synthesis and Antibacterial Action Mechanisms of Silver Nanoparticles
Silver nanoparticles (Ag NPs) represent one of the most widely employed metal-based engineered nanomaterials with a broad range of applications in different areas of science. Plant extracts (PEs) serve as green reducing and coating agents and can be exploited for the generation of Ag NPs. In this st...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-10-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/11/10/1415 |
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| author | Zaruhi Hovhannisyan Marina Timotina Jemma Manoyan Lilit Gabrielyan Margarit Petrosyan Barbara Kusznierewicz Agnieszka Bartoszek Claus Jacob Mikayel Ginovyan Karen Trchounian Naira Sahakyan Muhammad Jawad Nasim |
| author_facet | Zaruhi Hovhannisyan Marina Timotina Jemma Manoyan Lilit Gabrielyan Margarit Petrosyan Barbara Kusznierewicz Agnieszka Bartoszek Claus Jacob Mikayel Ginovyan Karen Trchounian Naira Sahakyan Muhammad Jawad Nasim |
| author_sort | Zaruhi Hovhannisyan |
| collection | DOAJ |
| description | Silver nanoparticles (Ag NPs) represent one of the most widely employed metal-based engineered nanomaterials with a broad range of applications in different areas of science. Plant extracts (PEs) serve as green reducing and coating agents and can be exploited for the generation of Ag NPs. In this study, the phytochemical composition of ethanolic extract of black currant (<i>Ribes nigrum</i>) leaves was determined. The main components of extract include quercetin rutinoside, quercetin hexoside, quercetin glucuronide, quercetin malonylglucoside and quercitrin. The extract was subsequently employed for the green synthesis of Ag NPs. Consequently, <i>R. nigrum</i> leaf extract and Ag NPs were evaluated for potential antibacterial activities against Gram-negative bacteria (<i>Escherichia coli</i> ATCC 25922 and kanamycin-resistant <i>E. coli</i> pARG-25 strains). Intriguingly, the plant extract did not show any antibacterial effect, whilst Ag NPs demonstrated significant activity against tested bacteria. Biogenic Ag NPs affect the ATPase activity and energy-dependent H<sup>+</sup>-fluxes in both strains of <i>E. coli</i>, even in the presence of <i>N,N’</i>-dicyclohexylcarbodiimide (DCCD). Thus, the antibacterial activity of the investigated Ag NPs can be explained by their impact on the membrane-associated properties of bacteria. |
| first_indexed | 2024-03-09T20:51:04Z |
| format | Article |
| id | doaj.art-dd6c9ca7583243f0970f096113924039 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-09T20:51:04Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-dd6c9ca7583243f0970f0961139240392023-11-23T22:36:04ZengMDPI AGAntibiotics2079-63822022-10-011110141510.3390/antibiotics11101415<i>Ribes nigrum</i> L. Extract-Mediated Green Synthesis and Antibacterial Action Mechanisms of Silver NanoparticlesZaruhi Hovhannisyan0Marina Timotina1Jemma Manoyan2Lilit Gabrielyan3Margarit Petrosyan4Barbara Kusznierewicz5Agnieszka Bartoszek6Claus Jacob7Mikayel Ginovyan8Karen Trchounian9Naira Sahakyan10Muhammad Jawad Nasim11Division of Bioorganic Chemistry, School of Pharmacy, Saarland University, 66123 Saarbruecken, GermanyDepartment of Medical Biochemistry and Biotechnology, Russian-Armenian University, 0051 Yerevan, ArmeniaDepartment of Biochemistry, Microbiology and Biotechnology, Biology Faculty, Yerevan State University, 0025 Yerevan, ArmeniaDepartment of Biochemistry, Microbiology and Biotechnology, Biology Faculty, Yerevan State University, 0025 Yerevan, ArmeniaDepartment of Biochemistry, Microbiology and Biotechnology, Biology Faculty, Yerevan State University, 0025 Yerevan, ArmeniaDepartment of Food Chemistry, Technology and Biotechnology, Faculty of Chemistry, Gdańsk University of Technology, 80-233 Gdańsk, PolandDepartment of Food Chemistry, Technology and Biotechnology, Faculty of Chemistry, Gdańsk University of Technology, 80-233 Gdańsk, PolandDivision of Bioorganic Chemistry, School of Pharmacy, Saarland University, 66123 Saarbruecken, GermanyDepartment of Biochemistry, Microbiology and Biotechnology, Biology Faculty, Yerevan State University, 0025 Yerevan, ArmeniaDepartment of Biochemistry, Microbiology and Biotechnology, Biology Faculty, Yerevan State University, 0025 Yerevan, ArmeniaDepartment of Biochemistry, Microbiology and Biotechnology, Biology Faculty, Yerevan State University, 0025 Yerevan, ArmeniaDivision of Bioorganic Chemistry, School of Pharmacy, Saarland University, 66123 Saarbruecken, GermanySilver nanoparticles (Ag NPs) represent one of the most widely employed metal-based engineered nanomaterials with a broad range of applications in different areas of science. Plant extracts (PEs) serve as green reducing and coating agents and can be exploited for the generation of Ag NPs. In this study, the phytochemical composition of ethanolic extract of black currant (<i>Ribes nigrum</i>) leaves was determined. The main components of extract include quercetin rutinoside, quercetin hexoside, quercetin glucuronide, quercetin malonylglucoside and quercitrin. The extract was subsequently employed for the green synthesis of Ag NPs. Consequently, <i>R. nigrum</i> leaf extract and Ag NPs were evaluated for potential antibacterial activities against Gram-negative bacteria (<i>Escherichia coli</i> ATCC 25922 and kanamycin-resistant <i>E. coli</i> pARG-25 strains). Intriguingly, the plant extract did not show any antibacterial effect, whilst Ag NPs demonstrated significant activity against tested bacteria. Biogenic Ag NPs affect the ATPase activity and energy-dependent H<sup>+</sup>-fluxes in both strains of <i>E. coli</i>, even in the presence of <i>N,N’</i>-dicyclohexylcarbodiimide (DCCD). Thus, the antibacterial activity of the investigated Ag NPs can be explained by their impact on the membrane-associated properties of bacteria.https://www.mdpi.com/2079-6382/11/10/1415silver nanoparticles<i>Ribes nigrum</i>natural productsphytochemical investigationantimicrobial |
| spellingShingle | Zaruhi Hovhannisyan Marina Timotina Jemma Manoyan Lilit Gabrielyan Margarit Petrosyan Barbara Kusznierewicz Agnieszka Bartoszek Claus Jacob Mikayel Ginovyan Karen Trchounian Naira Sahakyan Muhammad Jawad Nasim <i>Ribes nigrum</i> L. Extract-Mediated Green Synthesis and Antibacterial Action Mechanisms of Silver Nanoparticles Antibiotics silver nanoparticles <i>Ribes nigrum</i> natural products phytochemical investigation antimicrobial |
| title | <i>Ribes nigrum</i> L. Extract-Mediated Green Synthesis and Antibacterial Action Mechanisms of Silver Nanoparticles |
| title_full | <i>Ribes nigrum</i> L. Extract-Mediated Green Synthesis and Antibacterial Action Mechanisms of Silver Nanoparticles |
| title_fullStr | <i>Ribes nigrum</i> L. Extract-Mediated Green Synthesis and Antibacterial Action Mechanisms of Silver Nanoparticles |
| title_full_unstemmed | <i>Ribes nigrum</i> L. Extract-Mediated Green Synthesis and Antibacterial Action Mechanisms of Silver Nanoparticles |
| title_short | <i>Ribes nigrum</i> L. Extract-Mediated Green Synthesis and Antibacterial Action Mechanisms of Silver Nanoparticles |
| title_sort | i ribes nigrum i l extract mediated green synthesis and antibacterial action mechanisms of silver nanoparticles |
| topic | silver nanoparticles <i>Ribes nigrum</i> natural products phytochemical investigation antimicrobial |
| url | https://www.mdpi.com/2079-6382/11/10/1415 |
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