The flavohaemoprotein hmp maintains redox homeostasis in response to reactive oxygen and nitrogen species in Corynebacterium glutamicum
Abstract Background During the production of L-arginine through high dissolved oxygen and nitrogen supply fermentation, the industrial workhorse Corynebacterium glutamicum is exposed to oxidative stress. This generates reactive oxygen species (ROS) and reactive nitrogen species (RNS), which are harm...
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| Format: | Article |
| Language: | English |
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BMC
2023-08-01
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| Series: | Microbial Cell Factories |
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| Online Access: | https://doi.org/10.1186/s12934-023-02160-9 |
| _version_ | 1797555562432430080 |
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| author | Ziqin Jiang Jingyi Guan Tingting Liu Chunyu Shangguan Meijuan Xu Zhiming Rao |
| author_facet | Ziqin Jiang Jingyi Guan Tingting Liu Chunyu Shangguan Meijuan Xu Zhiming Rao |
| author_sort | Ziqin Jiang |
| collection | DOAJ |
| description | Abstract Background During the production of L-arginine through high dissolved oxygen and nitrogen supply fermentation, the industrial workhorse Corynebacterium glutamicum is exposed to oxidative stress. This generates reactive oxygen species (ROS) and reactive nitrogen species (RNS), which are harmful to the bacteria. To address the issue and to maintain redox homeostasis during fermentation, the flavohaemoprotein (Hmp) was employed. Results The results showed that the overexpression of Hmp led to a decrease in ROS and RNS content by 9.4% and 22.7%, respectively, and improved the survivability of strains. When the strains were treated with H2O2 and NaNO2, the RT-qPCR analysis indicated an up-regulation of ammonium absorption and transporter genes amtB and glnD. Conversely, the deletion of hmp gives rise to the up-regulation of eight oxidative stress-related genes. These findings suggested that hmp is associated with oxidative stress and intracellular nitrogen metabolism genes. Finally, we released the inhibitory effect of ArnR on hmp. The Cc-ΔarnR-hmp strain produced 48.4 g/L L-arginine during batch-feeding fermentation, 34.3% higher than the original strain. Conclusions This report revealed the influence of dissolved oxygen and nitrogen concentration on reactive species of Corynebacterium glutamicum and the role of the Hmp in coping with oxidative stress. The Hmp first demonstrates related to redox homeostasis and nitrite metabolism, providing a feasible strategy for improving the robustness of strains. |
| first_indexed | 2024-03-10T16:49:13Z |
| format | Article |
| id | doaj.art-dd6e9336b127473ba5c0f0976f26c9a4 |
| institution | Directory Open Access Journal |
| issn | 1475-2859 |
| language | English |
| last_indexed | 2024-03-10T16:49:13Z |
| publishDate | 2023-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj.art-dd6e9336b127473ba5c0f0976f26c9a42023-11-20T11:22:44ZengBMCMicrobial Cell Factories1475-28592023-08-0122111010.1186/s12934-023-02160-9The flavohaemoprotein hmp maintains redox homeostasis in response to reactive oxygen and nitrogen species in Corynebacterium glutamicumZiqin Jiang0Jingyi Guan1Tingting Liu2Chunyu Shangguan3Meijuan Xu4Zhiming Rao5The Key Laboratory of Industrial Biotechnology, School of Biotechnology, Ministry of Education, Jiangnan UniversityThe Key Laboratory of Industrial Biotechnology, School of Biotechnology, Ministry of Education, Jiangnan UniversityYantai Shinho Enterprise Foods Co., LtdThe Key Laboratory of Industrial Biotechnology, School of Biotechnology, Ministry of Education, Jiangnan UniversityThe Key Laboratory of Industrial Biotechnology, School of Biotechnology, Ministry of Education, Jiangnan UniversityThe Key Laboratory of Industrial Biotechnology, School of Biotechnology, Ministry of Education, Jiangnan UniversityAbstract Background During the production of L-arginine through high dissolved oxygen and nitrogen supply fermentation, the industrial workhorse Corynebacterium glutamicum is exposed to oxidative stress. This generates reactive oxygen species (ROS) and reactive nitrogen species (RNS), which are harmful to the bacteria. To address the issue and to maintain redox homeostasis during fermentation, the flavohaemoprotein (Hmp) was employed. Results The results showed that the overexpression of Hmp led to a decrease in ROS and RNS content by 9.4% and 22.7%, respectively, and improved the survivability of strains. When the strains were treated with H2O2 and NaNO2, the RT-qPCR analysis indicated an up-regulation of ammonium absorption and transporter genes amtB and glnD. Conversely, the deletion of hmp gives rise to the up-regulation of eight oxidative stress-related genes. These findings suggested that hmp is associated with oxidative stress and intracellular nitrogen metabolism genes. Finally, we released the inhibitory effect of ArnR on hmp. The Cc-ΔarnR-hmp strain produced 48.4 g/L L-arginine during batch-feeding fermentation, 34.3% higher than the original strain. Conclusions This report revealed the influence of dissolved oxygen and nitrogen concentration on reactive species of Corynebacterium glutamicum and the role of the Hmp in coping with oxidative stress. The Hmp first demonstrates related to redox homeostasis and nitrite metabolism, providing a feasible strategy for improving the robustness of strains.https://doi.org/10.1186/s12934-023-02160-9FlavohaemoproteinCorynebacterium glutamicumRedox homeostasisL-arginine |
| spellingShingle | Ziqin Jiang Jingyi Guan Tingting Liu Chunyu Shangguan Meijuan Xu Zhiming Rao The flavohaemoprotein hmp maintains redox homeostasis in response to reactive oxygen and nitrogen species in Corynebacterium glutamicum Microbial Cell Factories Flavohaemoprotein Corynebacterium glutamicum Redox homeostasis L-arginine |
| title | The flavohaemoprotein hmp maintains redox homeostasis in response to reactive oxygen and nitrogen species in Corynebacterium glutamicum |
| title_full | The flavohaemoprotein hmp maintains redox homeostasis in response to reactive oxygen and nitrogen species in Corynebacterium glutamicum |
| title_fullStr | The flavohaemoprotein hmp maintains redox homeostasis in response to reactive oxygen and nitrogen species in Corynebacterium glutamicum |
| title_full_unstemmed | The flavohaemoprotein hmp maintains redox homeostasis in response to reactive oxygen and nitrogen species in Corynebacterium glutamicum |
| title_short | The flavohaemoprotein hmp maintains redox homeostasis in response to reactive oxygen and nitrogen species in Corynebacterium glutamicum |
| title_sort | flavohaemoprotein hmp maintains redox homeostasis in response to reactive oxygen and nitrogen species in corynebacterium glutamicum |
| topic | Flavohaemoprotein Corynebacterium glutamicum Redox homeostasis L-arginine |
| url | https://doi.org/10.1186/s12934-023-02160-9 |
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