In vitro degradation of diacetoxyscirpenol and T-2 toxin by use of Mucor racemosus fresen. f. racemosus isolate
Under controlled in vitro conditions the capacity of the Mucor racemosus f. racemosus 1215/09 isolate to degrade type A trichothecenes (diacetoxyscirpenol - DAS and T-2 toxin) was observed in the liquid nutritive medium. According to previously performed experiments it was proved that the select...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Matica srpska
2011-01-01
|
| Series: | Zbornik Matice Srpske za Prirodne Nauke |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0352-4906/2011/0352-49061121051B.pdf |
| _version_ | 1818664584361279488 |
|---|---|
| author | Bočarov-Stančić Aleksandra S. Stanković Slavica Ž. Lević Jelena T. Salma Nataša M. Pantić Vladimir R. Barnić Saša S. |
| author_facet | Bočarov-Stančić Aleksandra S. Stanković Slavica Ž. Lević Jelena T. Salma Nataša M. Pantić Vladimir R. Barnić Saša S. |
| author_sort | Bočarov-Stančić Aleksandra S. |
| collection | DOAJ |
| description | Under controlled in vitro conditions the capacity of the Mucor racemosus f. racemosus 1215/09 isolate to degrade type A trichothecenes (diacetoxyscirpenol - DAS and T-2 toxin) was observed in the liquid nutritive medium. According to previously performed experiments it was proved that the selected isolate, originating from sunflower meal, had the ability to degrade these fusariotoxins when growing on the modified Vogel’s agar supplemented with crude extracts of DAS and T-2 toxin. In order to determine biodegradation of fusariotoxins, the liquid nutritive medium - SPY (5% sucrose + 0.1% peptone + 0.1% yeast extract, pH 6.2) was simultaneously inoculated with the isolate M. racemosus f. racemosus 1215/09 and: a) Fusarium semitectum SL-B (DAS producer) or b) F. sporotrichioides R-2301 (T-2 toxin producer). The SPY media, inoculated with single fungal isolates, were used as a control of toxin biosynthesis. The cultures were incubated at room temperature (21-26ºC) on the rotary shaker (175 rpm). After the 3-5-day incubation, the filtration of liquid cultures and the extraction of fusariotoxins from filtrates with ethyl-acetate were performed. Determinations of DAS and T-2 toxin were done by thin layer chromatography using silica gel G. Depending on the incubation duration, M. racemosus f. racemosus in the mixed culture with F. semitectum degraded from 90.0 to 99.97% of DAS present in the medium (40,000- 120,000 µg l-1), while in the mixed culture with F. sporotrichioides it degraded from 95.0 to 96.7% of T-2 toxin present in the medium (240,000 µg l-1). Sterile filtrates of mixed cultures and single culture of M. racemosus f. racemosus, obtained by passing liquid cultures through the 0.45-µm membrane filter and added to the SPY medium, did not affect degradation of type A trichothecenes that had been biosynthesised by isolates F. semitectum SL-B and F. sporotrichioides R-2301 in the liquid medium. |
| first_indexed | 2024-12-17T05:35:04Z |
| format | Article |
| id | doaj.art-dd6ee86deb8c429ea56c5cc585a370c0 |
| institution | Directory Open Access Journal |
| issn | 0352-4906 |
| language | English |
| last_indexed | 2024-12-17T05:35:04Z |
| publishDate | 2011-01-01 |
| publisher | Matica srpska |
| record_format | Article |
| series | Zbornik Matice Srpske za Prirodne Nauke |
| spelling | doaj.art-dd6ee86deb8c429ea56c5cc585a370c02022-12-21T22:01:39ZengMatica srpskaZbornik Matice Srpske za Prirodne Nauke0352-49062011-01-012011121515910.2298/ZMSPN1121051BIn vitro degradation of diacetoxyscirpenol and T-2 toxin by use of Mucor racemosus fresen. f. racemosus isolateBočarov-Stančić Aleksandra S.Stanković Slavica Ž.Lević Jelena T.Salma Nataša M.Pantić Vladimir R.Barnić Saša S.Under controlled in vitro conditions the capacity of the Mucor racemosus f. racemosus 1215/09 isolate to degrade type A trichothecenes (diacetoxyscirpenol - DAS and T-2 toxin) was observed in the liquid nutritive medium. According to previously performed experiments it was proved that the selected isolate, originating from sunflower meal, had the ability to degrade these fusariotoxins when growing on the modified Vogel’s agar supplemented with crude extracts of DAS and T-2 toxin. In order to determine biodegradation of fusariotoxins, the liquid nutritive medium - SPY (5% sucrose + 0.1% peptone + 0.1% yeast extract, pH 6.2) was simultaneously inoculated with the isolate M. racemosus f. racemosus 1215/09 and: a) Fusarium semitectum SL-B (DAS producer) or b) F. sporotrichioides R-2301 (T-2 toxin producer). The SPY media, inoculated with single fungal isolates, were used as a control of toxin biosynthesis. The cultures were incubated at room temperature (21-26ºC) on the rotary shaker (175 rpm). After the 3-5-day incubation, the filtration of liquid cultures and the extraction of fusariotoxins from filtrates with ethyl-acetate were performed. Determinations of DAS and T-2 toxin were done by thin layer chromatography using silica gel G. Depending on the incubation duration, M. racemosus f. racemosus in the mixed culture with F. semitectum degraded from 90.0 to 99.97% of DAS present in the medium (40,000- 120,000 µg l-1), while in the mixed culture with F. sporotrichioides it degraded from 95.0 to 96.7% of T-2 toxin present in the medium (240,000 µg l-1). Sterile filtrates of mixed cultures and single culture of M. racemosus f. racemosus, obtained by passing liquid cultures through the 0.45-µm membrane filter and added to the SPY medium, did not affect degradation of type A trichothecenes that had been biosynthesised by isolates F. semitectum SL-B and F. sporotrichioides R-2301 in the liquid medium.http://www.doiserbia.nb.rs/img/doi/0352-4906/2011/0352-49061121051B.pdfdiacetoxyscirpenol (DAS)T-2 toxinbiodegradationM. racemosus f. racemosusliquid medium |
| spellingShingle | Bočarov-Stančić Aleksandra S. Stanković Slavica Ž. Lević Jelena T. Salma Nataša M. Pantić Vladimir R. Barnić Saša S. In vitro degradation of diacetoxyscirpenol and T-2 toxin by use of Mucor racemosus fresen. f. racemosus isolate Zbornik Matice Srpske za Prirodne Nauke diacetoxyscirpenol (DAS) T-2 toxin biodegradation M. racemosus f. racemosus liquid medium |
| title | In vitro degradation of diacetoxyscirpenol and T-2 toxin by use of Mucor racemosus fresen. f. racemosus isolate |
| title_full | In vitro degradation of diacetoxyscirpenol and T-2 toxin by use of Mucor racemosus fresen. f. racemosus isolate |
| title_fullStr | In vitro degradation of diacetoxyscirpenol and T-2 toxin by use of Mucor racemosus fresen. f. racemosus isolate |
| title_full_unstemmed | In vitro degradation of diacetoxyscirpenol and T-2 toxin by use of Mucor racemosus fresen. f. racemosus isolate |
| title_short | In vitro degradation of diacetoxyscirpenol and T-2 toxin by use of Mucor racemosus fresen. f. racemosus isolate |
| title_sort | in vitro degradation of diacetoxyscirpenol and t 2 toxin by use of mucor racemosus fresen f racemosus isolate |
| topic | diacetoxyscirpenol (DAS) T-2 toxin biodegradation M. racemosus f. racemosus liquid medium |
| url | http://www.doiserbia.nb.rs/img/doi/0352-4906/2011/0352-49061121051B.pdf |
| work_keys_str_mv | AT bocarovstancicaleksandras invitrodegradationofdiacetoxyscirpenolandt2toxinbyuseofmucorracemosusfresenfracemosusisolate AT stankovicslavicaz invitrodegradationofdiacetoxyscirpenolandt2toxinbyuseofmucorracemosusfresenfracemosusisolate AT levicjelenat invitrodegradationofdiacetoxyscirpenolandt2toxinbyuseofmucorracemosusfresenfracemosusisolate AT salmanatasam invitrodegradationofdiacetoxyscirpenolandt2toxinbyuseofmucorracemosusfresenfracemosusisolate AT panticvladimirr invitrodegradationofdiacetoxyscirpenolandt2toxinbyuseofmucorracemosusfresenfracemosusisolate AT barnicsasas invitrodegradationofdiacetoxyscirpenolandt2toxinbyuseofmucorracemosusfresenfracemosusisolate |