Genomic Regions and Candidate Genes Affecting Response to Heat Stress with Newcastle Virus Infection in Commercial Layer Chicks Using Chicken 600K Single Nucleotide Polymorphism Array
Heat stress results in significant economic losses to the poultry industry. Genetics plays an important role in chickens adapting to the warm environment. Physiological parameters such as hematochemical parameters change in response to heat stress in chickens. To explore the genetics of heat stress...
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MDPI AG
2024-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/25/5/2640 |
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| author | Ying Wang Perot Saelao Ganrea Chanthavixay Rodrigo A. Gallardo Anna Wolc Janet E. Fulton Jack M. Dekkers Susan J. Lamont Terra R. Kelly Huaijun Zhou |
| author_facet | Ying Wang Perot Saelao Ganrea Chanthavixay Rodrigo A. Gallardo Anna Wolc Janet E. Fulton Jack M. Dekkers Susan J. Lamont Terra R. Kelly Huaijun Zhou |
| author_sort | Ying Wang |
| collection | DOAJ |
| description | Heat stress results in significant economic losses to the poultry industry. Genetics plays an important role in chickens adapting to the warm environment. Physiological parameters such as hematochemical parameters change in response to heat stress in chickens. To explore the genetics of heat stress resilience in chickens, a genome-wide association study (GWAS) was conducted using Hy-Line Brown layer chicks subjected to either high ambient temperature or combined high temperature and Newcastle disease virus infection. Hematochemical parameters were measured during three treatment phases: acute heat stress, chronic heat stress, and chronic heat stress combined with NDV infection. Significant changes in blood parameters were recorded for 11 parameters (sodium (Na<sup>+</sup>, potassium (K<sup>+</sup>), ionized calcium (iCa<sup>2+</sup>), glucose (Glu), pH, carbon dioxide partial pressure (PCO<sub>2</sub>), oxygen partial pressure (PO<sub>2</sub>), total carbon dioxide (TCO<sub>2</sub>), bicarbonate (HCO<sub>3</sub>), base excess (BE), and oxygen saturation (sO<sub>2</sub>)) across the three treatments. The GWAS revealed 39 significant SNPs (<i>p</i> < 0.05) for seven parameters, located on Gallus gallus chromosomes (GGA) 1, 3, 4, 6, 11, and 12. The significant genomic regions were further investigated to examine if the genes within the regions were associated with the corresponding traits under heat stress. A candidate gene list including genes in the identified genomic regions that were also differentially expressed in chicken tissues under heat stress was generated. Understanding the correlation between genetic variants and resilience to heat stress is an important step towards improving heat tolerance in poultry. |
| first_indexed | 2024-04-25T00:29:29Z |
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| language | English |
| last_indexed | 2024-04-25T00:29:29Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-dd6fb07bc785495ead7ecf6914ed4d4d2024-03-12T16:45:45ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672024-02-01255264010.3390/ijms25052640Genomic Regions and Candidate Genes Affecting Response to Heat Stress with Newcastle Virus Infection in Commercial Layer Chicks Using Chicken 600K Single Nucleotide Polymorphism ArrayYing Wang0Perot Saelao1Ganrea Chanthavixay2Rodrigo A. Gallardo3Anna Wolc4Janet E. Fulton5Jack M. Dekkers6Susan J. Lamont7Terra R. Kelly8Huaijun Zhou9Genomics to Improve Poultry Innovation Lab, University of California, Davis, CA 95616, USAGenomics to Improve Poultry Innovation Lab, University of California, Davis, CA 95616, USAGenomics to Improve Poultry Innovation Lab, University of California, Davis, CA 95616, USAGenomics to Improve Poultry Innovation Lab, University of California, Davis, CA 95616, USADepartment of Animal Science, Iowa State University, Ames, IA 50011, USAHy-Line International, Dallas Center, IA 50063, USADepartment of Animal Science, Iowa State University, Ames, IA 50011, USADepartment of Animal Science, Iowa State University, Ames, IA 50011, USAGenomics to Improve Poultry Innovation Lab, University of California, Davis, CA 95616, USAGenomics to Improve Poultry Innovation Lab, University of California, Davis, CA 95616, USAHeat stress results in significant economic losses to the poultry industry. Genetics plays an important role in chickens adapting to the warm environment. Physiological parameters such as hematochemical parameters change in response to heat stress in chickens. To explore the genetics of heat stress resilience in chickens, a genome-wide association study (GWAS) was conducted using Hy-Line Brown layer chicks subjected to either high ambient temperature or combined high temperature and Newcastle disease virus infection. Hematochemical parameters were measured during three treatment phases: acute heat stress, chronic heat stress, and chronic heat stress combined with NDV infection. Significant changes in blood parameters were recorded for 11 parameters (sodium (Na<sup>+</sup>, potassium (K<sup>+</sup>), ionized calcium (iCa<sup>2+</sup>), glucose (Glu), pH, carbon dioxide partial pressure (PCO<sub>2</sub>), oxygen partial pressure (PO<sub>2</sub>), total carbon dioxide (TCO<sub>2</sub>), bicarbonate (HCO<sub>3</sub>), base excess (BE), and oxygen saturation (sO<sub>2</sub>)) across the three treatments. The GWAS revealed 39 significant SNPs (<i>p</i> < 0.05) for seven parameters, located on Gallus gallus chromosomes (GGA) 1, 3, 4, 6, 11, and 12. The significant genomic regions were further investigated to examine if the genes within the regions were associated with the corresponding traits under heat stress. A candidate gene list including genes in the identified genomic regions that were also differentially expressed in chicken tissues under heat stress was generated. Understanding the correlation between genetic variants and resilience to heat stress is an important step towards improving heat tolerance in poultry.https://www.mdpi.com/1422-0067/25/5/2640heat stressGWASNDVchickenQTL |
| spellingShingle | Ying Wang Perot Saelao Ganrea Chanthavixay Rodrigo A. Gallardo Anna Wolc Janet E. Fulton Jack M. Dekkers Susan J. Lamont Terra R. Kelly Huaijun Zhou Genomic Regions and Candidate Genes Affecting Response to Heat Stress with Newcastle Virus Infection in Commercial Layer Chicks Using Chicken 600K Single Nucleotide Polymorphism Array International Journal of Molecular Sciences heat stress GWAS NDV chicken QTL |
| title | Genomic Regions and Candidate Genes Affecting Response to Heat Stress with Newcastle Virus Infection in Commercial Layer Chicks Using Chicken 600K Single Nucleotide Polymorphism Array |
| title_full | Genomic Regions and Candidate Genes Affecting Response to Heat Stress with Newcastle Virus Infection in Commercial Layer Chicks Using Chicken 600K Single Nucleotide Polymorphism Array |
| title_fullStr | Genomic Regions and Candidate Genes Affecting Response to Heat Stress with Newcastle Virus Infection in Commercial Layer Chicks Using Chicken 600K Single Nucleotide Polymorphism Array |
| title_full_unstemmed | Genomic Regions and Candidate Genes Affecting Response to Heat Stress with Newcastle Virus Infection in Commercial Layer Chicks Using Chicken 600K Single Nucleotide Polymorphism Array |
| title_short | Genomic Regions and Candidate Genes Affecting Response to Heat Stress with Newcastle Virus Infection in Commercial Layer Chicks Using Chicken 600K Single Nucleotide Polymorphism Array |
| title_sort | genomic regions and candidate genes affecting response to heat stress with newcastle virus infection in commercial layer chicks using chicken 600k single nucleotide polymorphism array |
| topic | heat stress GWAS NDV chicken QTL |
| url | https://www.mdpi.com/1422-0067/25/5/2640 |
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